Composite biological fresh-keeping agent for meat and preparation method of composite biological fresh-keeping agent
A technology of biological preservatives and preservatives, which is applied in the direction of using chemicals to preserve meat/fish, etc., can solve the problems such as the inability to effectively prolong the shelf life of chilled meat, and achieve the effects of ensuring nutrition and flavor, prolonging shelf life and simple operation.
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Embodiment 1
[0028] A compound biological preservative for meat, characterized in that the main components include sodium alginate, Lactobacillus helveticus fermented liquid and Lactobacillus casei fermented liquid, wherein Lactobacillus helveticus fermented liquid and Lactobacillus casei fermented liquid respectively account for the total amount of preservative The volume ratio is 55% and 45%; wherein sodium alginate accounts for 3% of the total mass of the above-mentioned Lactobacillus helveticus fermentation broth and Lactobacillus casei fermentation broth.
[0029] The preparation method of the compound biological preservative for meat is characterized in that the preparation process comprises the following steps:
[0030] After sterilizing the prepared two-part MRS medium, inoculate Lactobacillus helveticus and Lactobacillus casei respectively, culture at 32°C for 48 hours, centrifuge at 800r / min, take the supernatant to make Lactobacillus helveticus fermentation broth and The Lactoba...
Embodiment 2
[0033]A compound biological preservative for meat, characterized in that the main components include sodium alginate, Lactobacillus helveticus fermented liquid and Lactobacillus casei fermented liquid, wherein Lactobacillus helveticus fermented liquid and Lactobacillus casei fermented liquid respectively account for the total amount of preservative The volume ratios are 58% and 42%; wherein sodium alginate accounts for 4% of the total mass of the above-mentioned Lactobacillus helveticus fermentation broth and Lactobacillus casei fermentation broth.
[0034] The preparation method of the compound biological preservative for meat is characterized in that the preparation process comprises the following steps:
[0035] After sterilizing the prepared two-part MRS medium, inoculate Lactobacillus helveticus and Lactobacillus casei respectively, culture them at 34°C for 50 hours, centrifuge at 900r / min, take the supernatant to make Lactobacillus helveticus fermentation broth and The L...
Embodiment 3
[0038] A compound biological preservative for meat, characterized in that the main components include sodium alginate, Lactobacillus helveticus fermented liquid and Lactobacillus casei fermented liquid, wherein Lactobacillus helveticus fermented liquid and Lactobacillus casei fermented liquid respectively account for the total amount of preservative The volume ratio is 60% and 40%; wherein sodium alginate accounts for 5% of the total mass of the above-mentioned Lactobacillus helveticus fermentation broth and Lactobacillus casei fermentation broth.
[0039] The preparation method of the compound biological preservative for meat is characterized in that the preparation process comprises the following steps:
[0040] After sterilizing the prepared two-part MRS medium, inoculate Lactobacillus helveticus and Lactobacillus casei respectively, culture at 35°C for 53 hours, centrifuge at 1000r / min, take the supernatant to make Lactobacillus helveticus fermentation broth and The Lactob...
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