Method for extracting protein from sesame
A protein and sesame technology, which is applied in protein food ingredients, protein composition of vegetable seeds, food science, etc., can solve the problems of using organic solvents, long separation cycle, and complicated process, and achieve mild separation conditions, strong water solubility, Low dosage effect
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Embodiment 1
[0017] The method for extracting protein from sesame, its step is as follows:
[0018] 1. Weigh 100 grams of sesame seeds, grind them into fine powder through superfine grinding.
[0019] 2. Preparation of sesame protein extract: add 0.1mol / L of Na laurate containing 2% polyoxyethylene laurate to the sesame fine powder gained in step 1 2 HPO 4 1800ml of the aqueous solution was stirred and leached, and filtered after leaching for 1 hour to obtain the leaching solution of the sesame protein.
[0020] 3. Cloud point phase separation extraction: in two steps:
[0021] According to the solid-to-liquid ratio g / ml is 1:100, add lauric acid polyoxyethylene ether to the sesame protein extraction liquid obtained in step 2, fully mix to form a micellar solution, drop HCl solution in the micellar solution to adjust the pH value was 4.5, heated to a temperature of 80°C, and carried out phase-separation extraction to form a micellar phase and an aqueous phase, and centrifuged after 1 ho...
Embodiment 2
[0025] Except following difference, other is with embodiment 1.
[0026] 1. Weigh 100 grams of sesame seeds, grind them into fine powder through superfine grinding.
[0027] 2, preparation of sesame protein leaching solution: add the sodium laurate polyoxyethylene ether containing 4% in the sesame fine powder of step 1 gained 2 HPO 4 Aqueous solution 2000ml, carry out stirring leaching, filter after leaching for 2 hours, obtain the leaching solution of sesame protein.
[0028] 3. Cloud point phase separation extraction: in two steps:
[0029] Be 5:100 by solid-to-liquid ratio g / ml, add lauric acid polyoxyethylene ether in the sesame protein extraction liquid of step 2 gained, add lauric acid polyoxyethylene ether in the sesame protein extraction liquid of step 2 gained, Mix well to form a micellar solution, add 0.2mol / L HCL solution dropwise to the obtained micellar solution to adjust the pH to 4.6, heat to a temperature of 85°C, conduct phase separation extraction to form ...
Embodiment 3
[0033] Except following difference, other is with embodiment 1.
[0034] 1. Weigh 100 grams of black sesame seeds, grind them into fine powder through superfine grinding.
[0035] 2. Preparation of black sesame protein extract: add NaH containing 2% polyoxyethylene laurate to the black sesame fine powder obtained in step 1 2 PO 4 2000ml of aqueous solution was stirred and leached, filtered after leaching for 2 hours, and the leaching solution of black sesame protein was obtained.
[0036] 3. Cloud point phase separation extraction: in two steps:
[0037] According to the solid-to-liquid ratio g / ml is 3:100, add lauric acid polyoxyethylene ether to the sesamin extract solution obtained in step 2, fully mix to form a micellar solution, drop 0.1mol / ml in the obtained micellar solution Adjust the pH of the L HCL solution to 5.0 and heat it to a temperature of 85°C for phase-separation extraction to form a micelle phase and an aqueous phase, and centrifuge to separate the micell...
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