Five-ingredient botanical composition for treating hepatic fibrosis and preparation method thereof
A technology for liver fibrosis and botanical medicine, which is applied in the directions of drug combination, pharmaceutical formula, plant raw material, etc., can solve the problems of backward preparation and other problems, and achieve the effect of increasing efficacy and good application prospect.
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Embodiment 1
[0035]Anti-hepatic fibrosis curative effect of effective components of Fuzheng Huayu Compound:
[0036] 1.1 Animals:
[0037] 59 Wistar male rats, SPF grade, weighing 150±10 g, were provided by Shanghai Experimental Animal Center, Chinese Academy of Sciences, license number: SCXK (Shanghai): 2007-0005. All rats were raised in the SPF laboratory of the Experimental Animal Center of Shanghai University of Traditional Chinese Medicine, with normal diet and free drinking water.
[0038] 1.2 Main reagents:
[0039] Dimethylnitrosamine (DMN), purchased from Tokyo Chemical Industry Co., Ltd., batch number: MAL05; citric acid, analytically pure, batch number: F20080916; anhydrous sodium acetate, analytically pure, batch number: F20080714; trisodium citrate (Tri-sodium citrate), analytically pure, batch number: F20070110; 4-Dimethylamino benzaldehyde (4-Dimethylamino benzaldehyde), batch number: F20090516; Chloramines-T (chloramines-T), analytically pure, batch number: T20061130; Non...
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