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Complex microbial preparation for quickening tobacco aging and improving tobacco quality and application thereof

A technology of compound microorganisms and compound microorganisms, which is applied in the fields of application, tobacco, and tobacco treatment, can solve the problems of accelerating the aging of tobacco leaves, and achieve the effects of increasing the aging acidity, accelerating the aging speed, and improving the smoking taste of cigarettes.

Active Publication Date: 2013-07-17
HUBEI CHINA TOBACCO IND +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Microbes can degrade polysaccharides, proteins, nicotine, etc. in tobacco leaves, releasing amino acids and aroma substances, thereby accelerating the aging of tobacco leaves and improving the quality of cigarettes. The reported microorganisms include Bacillus subtilis, thermophilic actinomycetes, small cocci, Pseudomonas, etc., but no reports on Lactobacillus plantarum and Acetobacter

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] [1] Preparation of compound microbial preparation

[0026] (1) Culture medium preparation: take 20 g of tobacco leaves, add 160 g of water, reflux extraction for 2 hours, cool, filter, adjust the pH value to 3, add 10% glucose of solution quality, which is the culture medium;

[0027] (2) Activation of Lactobacillus plantarum: Add Lactobacillus plantarum CCTCC NO: M2012002 screened from stacked tobacco stems to the above-mentioned medium, culture at 25°C for 45 hours, centrifuge the bacterial solution at 4000r / min for 10min, take The serum was diluted so that the number of viable bacteria was not less than 5.0×10 9 cfu / ml;

[0028] (3) Activation of Thermoacetobacter: Add Thermoacetobacter CCTCC NO: M2012001 screened from stacked tobacco stems to the above-mentioned medium, culture at 30°C for 48 hours, centrifuge the bacterial solution at 4000r / min for 10min, take the above The serum was diluted so that the number of viable bacteria was not less than 5.0×10 9 cfu / ml...

Embodiment 2

[0037] [1] Preparation of compound microbial preparation

[0038] (1) Culture medium preparation: take 20 g of tobacco leaves, add 200 g of water, reflux extraction for 3 hours, cool, filter, adjust the pH value to 4, and add 20% glucose of the solution mass.

[0039](2) Activation of Lactobacillus plantarum: Add Lactobacillus plantarum CCTCC NO: M2012002 screened from stacked tobacco stems to the above-mentioned medium, culture at 30°C for 48 hours, centrifuge the bacterial solution at 4000r / min for 10min, take The serum was diluted so that the number of viable bacteria was not less than 5.0×10 9 cfu / ml.

[0040] (3) Activation of Thermoacetobacter: Add Thermoacetobacter CCTCC NO: M2012001 screened from stacked tobacco stems to the above-mentioned medium, culture at 30°C for 48 hours, centrifuge the bacterial solution at 4000r / min for 10min, take the above The serum was diluted so that the number of viable bacteria was not less than 5.0×10 9 cfu / ml.

[0041] (4) Proportio...

Embodiment 3

[0049] [1] Preparation of compound microbial preparation

[0050] (1) Culture medium preparation: take 20 g of tobacco leaves, add 240 g of water, reflux extraction for 3 hours, cool, filter, adjust the pH value to 5, and add 30% glucose of the solution mass.

[0051] (2) Activation of Lactobacillus plantarum: Add Lactobacillus plantarum CCTCC NO: M2012002 screened from stacked tobacco stems to the above-mentioned medium, cultivate at 35°C for 52 hours, centrifuge the bacterial solution at 4000r / min for 10min, take The serum was diluted so that the number of viable bacteria was not less than 5.0×10 9 cfu / ml.

[0052] (3) Activation of Thermoacetobacter: Add Thermoacetobacter CCTCC NO: M2012001 screened from stacked tobacco stems to the above-mentioned medium, culture at 30°C for 48 hours, centrifuge the bacterial solution at 4000r / min for 10min, take the above The serum was diluted so that the number of viable bacteria was not less than 5.0×10 9 cfu / ml.

[0053] (4) Propor...

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PUM

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Abstract

The invention discloses a complex microbial preparation for quickening tobacco aging and improving tobacco quality and application thereof, belonging to the technical field of microorganisms and application thereof. The complex microbial preparation contains lactobacillus plantarum PJH05CCTCC NO: M2012002 and clostriolium themoacidophilus PJH73CCTCC NO: M2012001, wherein the mass ratio of lactobacillus plantarum activate fluid to clostriolium themoacidophilus activate fluid is 1:0.5-2; and the viable count in the activate fluid is not less than 5.0*10<9> cfu / ml. The aging speed of tobacco is quickened by the complex microbial preparation, and micro-molecular sour ingredients such as acetic acid and lactic acid are produced during aging, so that tobacco aging sour flavor and soft fine smoke are increased. Meanwhile, the cigarette smoke is improved, and the cigarette irritation is reduced, so that the cigarette has better smoking mouthfeel.

Description

technical field [0001] The invention belongs to the technical field of microorganisms and their applications, and in particular relates to a compound microbial preparation and the application of the preparation to accelerate the aging of tobacco leaves and improve the smoking quality of tobacco leaves. Background technique [0002] Tobacco aging is an important process in tobacco processing, and aging is also an important link in the formation of tobacco smoking quality. The aging of tobacco leaves refers to a treatment method that changes the chemical composition and physical properties of tobacco leaves under natural temperature and humidity conditions, thereby improving its smoking quality. In the prior art, the research results of biological and chemical changes in the fermentation process of flue-cured tobacco and the results of smoking evaluation comprehensively show that the aging of flue-cured tobacco is mainly completed in 12-18 months. Under the existing technical...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A24B15/20
Inventor 潘家华黄龙庞登红
Owner HUBEI CHINA TOBACCO IND
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