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Application of tea extract product in biological prevention and control of aflatoxin

A technology of tea extract and aflatoxin, applied in application, food science, food preservation and other directions, can solve the problems of application limitation, poor lasting effect, etc., and achieve the effect of high safety, not easy to environmental change, and good application prospect.

Active Publication Date: 2013-07-24
SOUTH CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Plant extracts mainly include litsea cubeba essential oil, thyme oil, hippocampus tooth essential oil, rose essential oil, etc. These essential oils are volatile substances, which have a strong inhibitory effect on the growth of Aspergillus flavus and the production of toxins, but the persistence is poor ; Among microorganisms, lactic acid bacteria, bacillus, yellow yellow bacillus, myxobacteria, pseudomonas, Ralstia, Burkholderia, some yeasts and a small amount of fungi all have the ability to inhibit the growth and toxin production of Aspergillus flavus, But in the storage period of agricultural products or food processing, the application of these microorganisms is limited

Method used

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  • Application of tea extract product in biological prevention and control of aflatoxin
  • Application of tea extract product in biological prevention and control of aflatoxin
  • Application of tea extract product in biological prevention and control of aflatoxin

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Through the plate antibacterial experiment, the tea extracts were tested against Aspergillus flavus As3.4408 (China Microbial Culture Collection, the current number is CGMCC3.4408) and Aspergillus parasiticus As3.124 (China Microbial Culture Collection, the current number is CGMCC3 .124) Growth inhibition effect, the experimental research process is as follows:

[0026] (1) Preparation of tea extract: 500g each of green tea, oolong tea, black tea and dark tea were soaked in 5L boiling water for 20 minutes, then filtered, the filtrate was concentrated to remove 2 / 3 of the water, and then the remaining water was sublimated by freeze drying 4 kinds of tea water extracts.

[0027] (2) Preparation of PDA solid medium containing tea extract: Weigh 4 kinds of tea water extracts and dissolve them in distilled water to prepare a solution, of which 1g of green tea extract is dissolved in 1L of distilled water to obtain the concentration of green tea extract: In a 0.1% (m / v) solution,...

Embodiment 2

[0041] The experimental study on the inhibitory effect of green tea extract on the biosynthesis of aflatoxin in several agricultural products was carried out by inoculating Aspergillus flavus As3.4408 to peanuts, rice, soybeans, walnut kernels, almonds, and corn seeds to study the water soaking of green tea The effect of the extract on several agricultural products in inhibiting the biosynthesis of aflatoxin during the storage period.

[0042] The experimental method is as follows:

[0043] (1) Experimental materials and reagents:

[0044] Commercially available peanuts, rice, soybeans, walnut kernels, almonds and corn each 1kg;

[0045] Aflatoxin-producing strain: Aspergillus flavus AS3.4408 (now preserved in the China Collection of Microorganisms, the current number is CGMCC3.4408)

[0046] Green tea water extract (the preparation method is as described above);

[0047] (2) Inoculation and cultivation:

[0048] 1) Strain activation: the Aspergillus flavus AS3.4408 strain was inoculated...

Embodiment 3

[0056] The experimental study on the inhibitory effect of black tea extract on the biosynthesis of aflatoxin in several agricultural products was carried out by inoculating Aspergillus parasitica As3.124 (now stored in the China Microbial Culture Collection, the current number is CGMCC3.124) On peanuts, rice, soybeans, walnut kernels, almonds, and corn seeds, the effect of black tea water extract on inhibiting aflatoxin biosynthesis during storage of several agricultural products was studied.

[0057] The materials, reagents and methods are the same as in Example 1, except that the black tea water extract is used instead of the green tea water extract (the extraction method is also the same), and another toxin-producing Aspergillus flavus strain Aspergillus parasiticus AS3.124 is used for the experiment. The effect of tea extract on the biosynthesis of aflatoxins by Aspergillus parasitica AS3.124 in different agricultural products.

[0058] See the results of the experiment Figure...

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Abstract

The invention belongs to food biotechnology field, and discloses an application of a tea extract product in biological prevention and control of aflatoxin, and concretely relates to an new application of the tea extract product for inhibiting the growth of producing bacteria of aflatoxin and biosynthesis of aflatoxin, the tea extract product comprises the tea extract product through a conventional extraction method through green tea, black tea and dark green tea, and the tea extract product has obvious effect for inhibiting the growth of producing bacteria of aflatoxin and has obvious effect for inhibiting the biosynthesis of aflatoxin. In the practical application, the tea extract product can be used for prevention and control of aflatoxin in a storage process of food and feed, and the effect is obvious.

Description

Technical field [0001] The invention belongs to the field of food biotechnology, and relates to new uses of tea extracts. Specifically, it relates to the application of tea extracts in the biological control of aflatoxins, and specifically is that the tea extracts inhibit the growth of aflatoxin-producing bacteria And its application in inhibiting the biosynthesis of aflatoxin. Background technique [0002] Aflatoxins are a kind of mycotoxins that are highly carcinogenic to humans, livestock and poultry. They are the general term for a class of compounds with similar structures. According to their molecular structures, naturally-occurring aflatoxins are divided into B1 and B2 , G1, G2. Aspergillus flavus and A. parasiticus are the two main fungi that produce aflatoxins. The toxicity test proved that B1 was the most toxic, followed by: M1>G1>B2>M2>G2. As AFB1 is known to be the most carcinogenic mycotoxin and has been studied the most, the commonly referred to as af...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L3/3472
Inventor 方祥谭英智周建良李斌区建发陈耀旭陈柱涛
Owner SOUTH CHINA AGRI UNIV
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