A DNA molecular identification method for Luchuan pig and its meat products

A DNA molecule and identification method technology, which is applied in the field of DNA molecular identification of Luchuan pigs and their meat products, achieves the effects of rich polymorphism, improved detection efficiency and accuracy, and simple operation

Active Publication Date: 2015-10-28
GUANGXI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But there is no report on the application of DNA molecular technology to distinguish and identify Luchuan pig breeds at home and abroad

Method used

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  • A DNA molecular identification method for Luchuan pig and its meat products
  • A DNA molecular identification method for Luchuan pig and its meat products
  • A DNA molecular identification method for Luchuan pig and its meat products

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Identify and distinguish Luchuan pig and its meat products from binary miscellaneous pigs, triple miscellaneous pigs and their meat products

[0036] Several samples of raw meat, bacon and buckwheat from Luchuan pig, binary mixed pig, and triple mixed pig were selected for identification, and the DNA of each sample was extracted by the improved SDS method.

[0037]Using WM14 as a primer, the total DNA of each group of samples was amplified through ISSR-PCR reaction. The ISSR-PCR reaction system is 25 μL system: template DNA 1 μL, primer 2 μL, 2×Taq PCR Master Mix 12.5 μL, ddH 2 O9.5μL, in which 2×Taq PCR Master Mix contains Taq DNA polymerase, dNTPs, MgCl 2 , reaction buffer, PCR reaction enhancer, optimizer and stabilizer, the concentration is 2×, and does not contain dye; amplification program: 95°C pre-denaturation for 5min, 95°C denaturation for 30s, 36°C annealing for 30s, 72°C extension 2min, cycled 30 times, total extension at 72°C for 5min. Among them, Marker...

Embodiment 2

[0044] Identification and distinction of Luchuan pig and its meat products from fresh meat of Huanjiang Xiang pig and its meat products

[0045] Several fresh meat samples of Luchuan pig, Huanjiang Xiang pig and their bacon were selected to be identified, and the DNA of each sample was extracted by the improved SDS method.

[0046] Using WM12 as a primer, the ISSR-PCR amplification was the same as in Example 1.

[0047] Use 1.5% agarose gel and 1×TAE buffer as the medium to electrophoresis to detect the amplification result, draw the electrophoresis pattern 4 and 5 according to the electropherogram, and use Figure 4 The electrophoretic pattern drawn based on Figure 9 . As shown by the arrows in each figure, a relatively clear band was produced in Luchuan pig samples, but there was no such band in Huanjiang Xiang pigs. Repeated verification experiments can prove that the primer WM12 can be used to distinguish Luchuan pigs from Huanjiang pigs. Strong evidence for the Jiangx...

Embodiment 3

[0053] Identification and distinction of Luchuan pig and its meat products from Bamaxiang pig fresh meat and its meat products

[0054] Several samples of Luchuan pig, Bamaxiang pig fresh meat and their braised meat samples were selected, and the DNA of each sample was extracted by the improved SDS method.

[0055] Using WM29 as a primer, the ISSR-PCR amplification was the same as in Example 1.

[0056] Use 1.5% agarose gel and 1×TAE buffer as the medium to electrophoresis to detect the amplification results, draw the electrophoretic patterns 6 and 7 according to the electrophoretic graphs, and use Figure 7 The electrophoretic pattern drawn based on Figure 10 . As shown by the arrows in each figure, a relatively clear band was produced in the samples of Bamaxiang pigs, but there was no such band in Luchuan pigs. Repeated verification experiments can prove that the primer WM29 can be used to distinguish Luchuan pigs from Bama pigs. Strong evidence for the horse-scented pig...

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Abstract

The invention relates to a DNA (Deoxyribonucleic Acid) molecular identification method for Luchuan pigs and meat products thereof. According to the method, primers used in ISSR-PCR (Inter-Simple Sequence Repeat-Polymerase Chain Reaction) are WM14, WM12 and WM29, and nucleotide sequences of the primers are 5'-CTCCTCCTCGC-3', 5'-CACCACCACGC-3' and 5'-CTCCTCCTCAT-3'; and finally, seven electrophoretograms of the Luchuan pigs and the meat products thereof are drawn, a DNA fingerprint atlas of the Luchuan pigs and the meat products thereof is established, and the electrophoretograms and the DNA fingerprint atlas can serve as a basis for the distinction and identification of the Luchuan pigs and the meat products thereof. The method has the advantages that a reliable DNA molecular identification technique is adopted, so that the differences among the Luchuan pigs, the meat products of the Luchuan pigs, Luchuan pig hybrids, meat products of the Luchuan pig hybrids, Bama miniature pigs, meat products of the Bama miniature pigs, Huanjiang miniature pigs and meat products of the Huanjiang miniature pigs are identified quickly and accurately, and the development of advantaged brand pigs, namely the Luchuan pigs, is promoted.

Description

technical field [0001] The invention relates to a method for identifying pig breeds using DNA molecular marker technology, in particular to a DNA molecular identification method for Luchuan pigs and meat products thereof. Background technique [0002] Luchuan pig is an excellent pig breed in South China. It is named for its origin in Luchuan County in the southeast of Guangxi Province. Now it is mainly distributed in Yulin, Wuzhou, Qinzhou and other regions in Guangxi. Together with Rongchang pig, Taihu pig, Northeast Min pig, Jinhua pig, Ningxiang pig, Huai pig and Bamei pig, Luchuan pig is called "China's eight local excellent pig breeds". Luchuan pig is a small fat pig breed, which has long been famous for its thin skin, tender meat, crisp but not greasy, delicious taste and rich nutrition. Its livestock products can be processed into sausages, skinless streaky bacon, white-cut pig feet, crispy suckling pigs, braised pork and other products, which are favored by local co...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/68
Inventor 韦保耀王淼黄丽滕建文夏宁王勤志封毅
Owner GUANGXI UNIV
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