Method for preparing cyanidenon-7-O-beta-D-glucuronide

Abstract

The invention discloses an efficient, simple and convenient method for preparing cyanidenon-7-O-beta-D-glucuronide. The efficient, simple and convenient method comprises the following steps of: a. smashing an irexis sonchifolia raw material, soaking in 5-10 times amount of saturated lime water for 2-3 times, mixing extracting solutions, filtering, carrying out molecule trapping sequentially by an ultrafiltration membrane and a nanofiltration membrane, adjusting pH of trapped liquid to 3-5, absorbing the trapped liquid by polyamide resin, carrying out gradient elution by an ethanol solution, and concentrating eluent to obtain a concentration solution; and b. purifying the concentration solution by adopting high-speed counter-current chromatography, carrying out online monitoring by an ultraviolet detector, collecting a target component according to an atlas, recovering the reagent, and drying under reduced pressure to obtain the catalpol. The method for preparing the cyanidenon-7-O-beta-D-glucuronide has the advantages that the process operation is simple, the product yield is high, and the industrial amplification is easy.

Description

technical field [0001] The invention belongs to the field of natural medicinal chemistry, in particular to a method for preparing luteolin-7-O-β-D-glucuronide. Background technique [0002] Luteolin-7-O-β-D-glucuronide is a flavonoid glycoside based on luteolin, molecular formula C 21 h 18 o 12 , the molecular formula [0003] . [0004] Luteolin-7-O-β-D-glucuronide has physiological activities such as treating cardiovascular and cerebrovascular diseases and anti-tumor. [0005] Through literature search, the existing methods for preparing luteolin-7-O-β-D-glucuronide are relatively cumbersome, especially for high-purity products, and are difficult to produce industrially. For example, the patent (Application No.: 201110328655.8) "Preparation, quality control method and use of a Kudiezi active ingredient composition", the method disclosed in the patent is that the Kudiezi is first pretreated by enzymatic method, and then supercritical fluid extraction method is used....

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Problems solved by technology

[0005] Through literature search, the existing methods for preparing luteolin-7-O-β-D-glucuronide are relatively cumbersome, especially for high-purity products, and it is difficult to industrialize production