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Application of SOAR1 protein and coding gene thereof to regulation and control on tolerance of plants to abscisic acid (ABA)

A technology for encoding genes and transgenic plants, which is applied in the application field of SOAR1 protein and its encoding genes in regulating plant tolerance to ABA, which can solve problems such as the complexity of ABA signaling pathways, and achieve the effect of improving genetic characteristics

Inactive Publication Date: 2013-08-07
TSINGHUA UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Current studies indicate that the ABA signaling pathway mediated by ABAR is very complex

Method used

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  • Application of SOAR1 protein and coding gene thereof to regulation and control on tolerance of plants to abscisic acid (ABA)
  • Application of SOAR1 protein and coding gene thereof to regulation and control on tolerance of plants to abscisic acid (ABA)
  • Application of SOAR1 protein and coding gene thereof to regulation and control on tolerance of plants to abscisic acid (ABA)

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] Embodiment 1, the acquisition and identification of SOAR1 transgenic plants

[0059] The SOAR1 gene involved in this example is derived from Arabidopsis thaliana, and its sequence in the Arabidopsis genome is shown as sequence 1 in the sequence listing. Sequence 1 consists of 2216 nucleotides, of which the 653rd Position -763 is an intron sequence; the cDNA sequence of the SOAR1 gene is shown in sequence 2 in the sequence table, sequence 2 is composed of 2105 nucleotides, and the 89th-1897th position is the coding sequence (ORF); sequence 1 Both Sequence 2 and Sequence 2 encode the protein shown in Sequence 3 in the sequence listing, and Sequence 3 consists of 602 amino acid residues.

[0060] According to bioinformatics prediction, SOAR1 is a member of the PPR protein family.

[0061] exist http: / / www.ebi.ac.uk / Tools / InterProScan / index.htmla The SOAR1 protein was predicted in the database, and it was found that the SOAR1 protein was composed of an N-terminal signal ...

Embodiment 2

[0140] Example 2, SOAR1 Transgenic Plants Tolerance Analysis Test to ABA

[0141] 1. Effect of ABA on seed germination of SOAR1 genetic materials

[0142] ABA plays a vital role in the process of plant seed dormancy and germination, and exogenous ABA can inhibit the normal germination of seeds. With the increase of exogenous ABA concentration, the germination rate of Arabidopsis wild-type (Col-0 ecotype) seeds gradually decreased. Seed germination experiment is one of the important methods to study ABA signal transduction, and the effect of ABA on seed germination of SOAR1-related mutant plants can be observed.

[0143] The single-copy inserted T3 generation homozygous SOAR1 transgene obtained from Arabidopsis wild type (Col-0 ecotype), two T-DNA insertion mutants soar1-2 and soar1-3 with reduced SOAR1 gene expression, and Example 1 The strains OE1, OE3 and OE6, and the control plants obtained in Example 1 transformed into the pCAMBIA-1300-221 empty vector were used as exper...

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Abstract

The invention discloses an application of SOAR1 protein and a coding gene thereof to regulation and control on the tolerance of plants to abscisic acid (ABA). The application provided by the invention is particularly the application of protein consisting of the amino acid sequence as shown in the sequence 3 in a sequence table to the following a1) or a2): a1) regulating and controlling the tolerance of the plants to the ABA; and a2) breeding the plant varieties with improved tolerance to the ABA. According to the application, the plant hormone ABA serves as a selective herbicide, the selectivity depends on the sensitivity of the plants to the ABA, and the SOAR1 gene is used for regulating the sensitivity or tolerance of the plants to the ABA, so that SOAR1 gene high-expression and herbicide-resistant transgenic crops are obtained, and selective weeding is realized. The application accords with the requirement of sustainable agriculture development and has important practical value and market prospect in the aspects of developing an environment-friendly pollution-free weeding method and path and the like.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to the application of a SOAR1 protein and its coding gene in regulating plant tolerance to ABA. Background technique [0002] Abscisic acid (ABA) is the most important natural growth hormone in plants. It was discovered as early as the early 1960s. It is named for its ability to promote the shedding of plant leaves. The plant hormone ABA participates in the regulation of various stages of plant growth and development, including seed dormancy, germination, seedling growth, lateral root growth, stomatal movement, and the transition from vegetative growth to reproductive growth. At the same time, ABA plays an important role in the response of plants to a variety of external stresses, including abiotic stresses such as drought, cold, salt, osmotic stress, and mechanical injury, as well as biotic stresses such as pests and diseases. Due to the limitations of the growth and development of plan...

Claims

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Application Information

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IPC IPC(8): C07K14/415C12N15/29C12N15/82A01H5/00A01N37/42A01P13/00
Inventor 张大鹏梅超姜上川王小芳
Owner TSINGHUA UNIV
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