Hybridoma cell strain secreting high-neutralization activity infectious bursal disease virus (IBDV) monoclonal antibody

A monoclonal antibody, hybridoma cell technology, applied in the field of hybridoma cells and antibody engineering, can solve the problems of low neutralizing titer, no report on neutralizing activity, etc. Reduced, good stability
CN103232974BActive Publication Date: 2014-09-03JIANGSU ACAD OF AGRI SCI
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
JIANGSU ACAD OF AGRI SCI
Publication Date
2014-09-03
Patent Text Reader

Abstract

The invention relates to a hybridoma cell strain secreting high neutralizing active infectious bursal virus (IBDV) monoclonal antibody, which belongs to the field of biotechnology. The present invention screens a hybridoma cell C128 strain from the established hybridoma cell library secreting IBDV monoclonal antibody, and the mouse ascites monoclonal antibody prepared by it has a neutralizing titer of up to 1010 against IBDV. The hybridoma cell C128 strain of the present invention secretes a high neutralizing active IBDV monoclonal antibody not only has high neutralizing titer but also has a wide range of anti-IBDV strains, and is used for the clinical treatment of infectious bursal disease (IBD) animals with remarkable curative effect. Safe and no adverse side effects. The monoclonal antibody therapeutic agent prepared by the hybridoma cell C128 strain of the present invention has good stability and does not decrease in neutralization titer after being stored for two years.
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Description

technical field

[0001] The invention relates to a hybridoma cell secreting high neutralizing active infectious bursal virus (IBDV) monoclonal antibody, belongs to the field of biotechnology, and relates to antibody engineering technology. Specifically, the monoclonal antibody hybridoma C128 strain has excellent production performance, and the secreted monoclonal antibody not only has high neutralizing titer but also has high neutralizing activity against IBDV strains from different animal sources, and can be used for infectious bursae Clinical treatment of animals with IBD. Background technique

[0002] Infectious Bursal Disease (IBD) is caused by Infectious Bursal Disease Virus (IBDV). characteristic infectious disease. The disease not only causes the death of sick animals, but also leads to immunosuppression of the body, which reduces the immune defense ability of the body and the failure of vaccination. It is one of the main infectious diseases that cause serious econom...

Claims

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