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Method for extracting total RNA from leucaena leucocephala leaf tissue

A technology of Leucaena Leucaena leaves, which is applied in the field of extracting total RNA from Leucaena Leucaena leaf tissue, can solve problems such as inability to extract total RNA, and achieve the effects of strong promotion and application value, strong practicability, and good integrity

Inactive Publication Date: 2015-04-22
ANALYSIS & TESTING CENT CHINESE ACADEMY OF TROPICAL AGRI SCI
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Problems solved by technology

[0004] The present invention provides a method for extracting total RNA from Leucaena leaf tissue, aiming at solving the problem that total RNA cannot be extracted from Leucaena leaf at present

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  • Method for extracting total RNA from leucaena leucocephala leaf tissue
  • Method for extracting total RNA from leucaena leucocephala leaf tissue

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Embodiment Construction

[0032] In order to make the purpose, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the accompanying drawings and embodiments. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the invention.

[0033] figure 1 It shows the implementation process of the method for extracting total RNA in Leucaena leaf tissue provided by the embodiment of the present invention.

[0034] The method includes the following steps:

[0035] In step S101, fully shake the cetyltrimethylammonium bromide (CTAB) extract, take 15mL of the extract in a 50mL centrifuge tube, and preheat it in a 65°C water bath;

[0036] In step S102, take 2.0-3.0 g of Leucaena leaves and grind them into fine powder in liquid nitrogen, transfer to a centrifuge tube filled with preheated cetyltrimethylammonium bromide (CTAB) extract, and A...

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Abstract

The invention discloses a method for extracting total RNA from leucaena leucocephala leaf tissues. During a leucaena leucocephala leaf tissue total RNA extraction process, 0.9-1.0g of crosslinked polyvinyl pyrrolidone (PVPP) powder is added, such that polysaccharides, proteins, and transparent viscous insoluble substances are comprehensively removed. With the method, the OD260 / OD280 ratio of the extracted leucaena leucocephala leaf tissue total RNA is 1.8-2.0. The obtained RNA has high purity and high yield. Also, when the obtained RNA extract liquid is detected by using 1.5% agarose gel denaturation electrophoresis, the obtained strip is clear, RNA integrity is good, and the extracted RNA can be completely used in leucaena leucocephala molecular biology researches. The method has important significance in analyzing leucaena leucocephala gene expression and regulation, gene cloning and function identification, and molecular marker-assisted breeding. The method has high practicality, and good promotion ad application value.

Description

technical field [0001] The invention belongs to the technical field of biological genes, in particular to a method for extracting total RNA in Leucaena leaf tissue. Background technique [0002] Leucaena is native to the Americas and is an important tropical and subtropical woody leguminous forage and fast-growing forest tree species. It can be used for feed, green manure, fuel, forest and phytoremediation, water and soil conservation, cultivation of edible fungi, health care and medicine, etc. , is praised by the feed experts of the Food and Agriculture Organization of the United Nations as the "protein warehouse" and "miracle tree" in arid areas. In 1957, my country began to carry out research on the introduction and cultivation of Leucaena, and selected and bred "Reyan No. 1 Leucaena". At present, Leucaena psyllid has become the most important pest affecting the planting and utilization of Leucaena. Breeding of Leucaena psyllids for resistance has become an urgent probl...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N15/10
Inventor 邹冬梅蒋昌顺申建斌吴成贡
Owner ANALYSIS & TESTING CENT CHINESE ACADEMY OF TROPICAL AGRI SCI
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