Simple device and method for collecting volatile gas generated by bacteria
A volatile gas and collection device technology, applied in the biological field, can solve the problem that trace gases cannot be enriched at high concentrations, and achieve the effects of simple manufacturing process, promotion of full contact, and simple operation
Inactive Publication Date: 2013-08-14
JIANGSU UNIV
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[0004] The purpose of the present invention is to design a simple device specially used for collecting volatile gas in the bacterial culture process, effectively solve the problem that trace gas cannot be enriched at high concentration in a closed container, and combine it with air pump blowing and activated carbon adsorption , to establish a simple, easy-to-operate, low-cost volatile gas enrichment method for chemical analysis of volatile gas components produced by microbial metabolism
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[0017] The bacterial culture system 11 of the present embodiment is a 250 ml wide-mouthed Erlenmeyer flask, about 100 ml of mineral salt culture medium, inoculated bacteria with 2% inoculum, cultivated at 30 ° C, and the mouth of the Erlenmeyer flask is connected with an air inlet pipe 3 and The rubber piston 12 of the air outlet pipe 6. The volatile gas collection device includes a collection tube 9 with a standard plug 8 and an adjustment piston 10 at the bottom. The collection tube 9 is filled with 400 mg of activated carbon. When collecting the gas, the piston is opened so that the gas can contact with the activated carbon, and then the gas can be contacted with the activated carbon as soon as possible. Possible adsorption of volatile gases. When collecting volatile gases, activated carbon was soaked in dichloromethane for 30 min, and the extraction was repeated three times. Then the dichloromethane eluate was filtered through a 0.22 μm oil membrane, and then GC-MS analys...
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Abstract
The invention relates to a device and method for collecting a volatile gas generated by bacteria. The device mainly comprises an air pump, bacteria and a gas collecting device, wherein the air pump is used as a gas supplying device, is capable of maximally discharging the volatile gas generated by the bacteria in a cultivating gas producing system to a gas collecting pipe and being in full contact with absorbing material active carbon filled in the gas collecting pipe so that the volatile gas is efficiently absorbed and enriched, and is also capable of providing sufficient oxygen for bacteria to ensure that the bacteria keep high metabolic activity to further promote the generation of the volatile gas. The device is simple and reliable in structure, is capable of avoiding pollution of gas transferring, is safe and convenient to operate and low in cost, effectively realizes efficient real-time enrichment of the volatile gas generated by the bacteria, and brings the convenience for analyzing varieties of volatile materials generated by microorganisms in a metabolic process. According to the device, the problems that the volatile metabolic gas generated by the bacteria can not be effectively enriched in a container, the volatile gas is easily polluted in a transferring and collecting process, and the like are solved.
Description
technical field [0001] The invention designs a simple device and method for efficiently collecting volatile gases from bacteria, improves the enrichment efficiency of volatile gases produced by bacteria, and belongs to the field of biotechnology. Background technique [0002] For half a century, the control of plant diseases by chemical pesticides has played an important role in agricultural production, but the long-term irrational use of chemical pesticides has caused many problems. The high toxicity and high residue of chemical pesticides not only cause harm to human and livestock health, but also cause serious pollution to soil, water, and atmosphere, and destroy the ecological balance. At the same time, due to the increasing drug resistance of bacteria and pests, the amount and frequency of pesticide use are increasing, and a vicious circle of increasing amounts of pesticides and diseases has emerged, posing a great threat to the safety of humans and animals. A series o...
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IPC IPC(8): C12M1/107C12M1/04C12N1/20B01D53/04
CPCY02E50/343C12M23/36C12M21/04Y02E50/30
Inventor 王明娜肖翔杜道林戴志聪王莉莉
Owner JIANGSU UNIV