Electrochemistry gene chip

Abstract

The invention relates to the technical field of gene detection, and more specifically relates to an electrochemical gene chip. It includes a fluid chamber, a channel module, and a probe circuit board that fits with the channel module. The channel module is provided with a micro-flow groove. The contact forms a microfluidic pipeline, and the fluid chamber communicates with the microfluidic pipeline. Along the microfluidic pipeline, the probe circuit board is provided with a reference electrode for providing a reference voltage for electrochemical voltammetry scanning, and an auxiliary electrode for communicating with the electrochemical excitation circuit. An electrode and a working electrode for connecting a detection circuit, and a monomolecular layer and a DNA capture probe are fixed on the working electrode. One or more working electrodes of the present invention can be set according to actual needs, so that the hybridization solution can hybridize with the capture probes fixed on multiple working electrodes during the flow process after entering the microfluidic pipeline from the fluid chamber, which can be completed at one time. The detection of multiple indicators greatly improves the efficiency of detection.

Description

technical field [0001] The invention relates to the technical field of gene detection, and more specifically, to an electrochemical gene chip. Background technique [0002] Gene chips, also called DNA chips, are about the size of a fingernail. The base surface of each chip can be divided into tens of thousands to millions of cells. In the designated cells, a large number of nucleic acid molecules with specific functions and a length of about 20 base sequences can be immobilized. The nucleic acid molecules are also called molecular probes. [0003] Using the principle of molecular hybridization and parallel processing, the gene chip can carry out molecular detection of genetic material, which can be used in gene research, forensic identification, disease detection and drug screening, etc. The characteristics of intensification and low cost are a major innovation and leap in traditional biotechnology such as detection, hybridization, typing and DNA sequencing technology. Du...

AI Technical Summary

Problems solved by technology

[0006] 1. When it is necessary to detect multiple indicators of a liquid, it is necessary to test multiple samples of the liquid. Since different indicators correspond to different samples, the accuracy of the test results will be greatly weakened.

[0007] 2. Since multiple indicators of the same liquid require multiple samples to be tested multiple times, the amount of samples and reagents is large, the operation is complicated and time-consuming, and the repeatability is poor

[0008] 3. When multiple or multiple indicators are detected on the same sample tissue, manual operation is required, which not only has a low degree of automation, but also easily contaminates the sample

[0009] 4. For a gene chip, because it has been in contact with different samples of the same liquid, other biological reactions may occur, resulting in contamination and affecting the test results

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