Fungal agent for prevention and control of watermelon fusarium wilt and preparation method thereof

Abstract

Belonging to the technical field of biological prevention and control of soil borne diseases of melons, the invention discloses a fungal agent for prevention and control of watermelon fusarium wilt and a preparation method thereof. The method includes: preparation of mediums of different levels and preparation of an adsorption carrier; slant strain culture; shake flask strain propagation; production fermentation tank culture; solid agent preparation and other steps. In the invention, a Hypocrea atroviridis strain 2-22CGMCCNo.7128 is taken as the strain, which is prepared into a liquid and / or solid agent by fermentation, so that the attack time of watermelon fusarium wilt can be delayed, and the incidence can be reduced by over 40%. After watermelons undergo a continuous second crop, a good disease prevention effect can still be maintained. The agent provided in the invention has the advantages of rich raw materials, simple process, low cost, and a shelf life of more than half a year, and can be popularized and applied in watermelon planting areas.

Description

technical field [0001] The invention relates to the technical field of biological control of melon soil-borne diseases, in particular to a fungal agent for preventing and treating watermelon wilt and a preparation method thereof. Background technique [0002] Fusarium wilt of watermelon is caused by Fusarium oxysporum ( Fusarium oxysporum f.sp.niveum ) caused by a common soil-borne disease, the disease is widely spread around the world, my country's Taiwan, Guangdong, Zhejiang, Fujian, Jiangsu, Hubei, Sichuan, Chongqing and other regions seriously occurred. The disease can cause large areas of wilting and death of plants. The incidence rate of general fields is 10% to 30%, and the incidence rate of severe disease fields is as high as 80%, or even all of them die, resulting in a serious decline in watermelon production and major economic losses. [0003] Fusarium wilt of watermelon is the most common disease in watermelon continuous cropping production, with the grea...

AI Technical Summary

Problems solved by technology

The use of microorganisms to control crop soil-borne diseases has been studied for decades, and people have successively reported Bacillus subtilis, Agrobacterium radioactive, Pseudomonas fluorescens, Arthrobacter, Bacillus polymyxa, Trichoderma fungus, Qing Mold, etc.; At present, there are many products such as microbial agents and bacterial fertilizers that utilize antagonistic bacteria to prevent and treat plant soil-borne diseases on the market, and there are also some test reports that utilize products such as biological bacterial fertilizers to carry out biological control of watermelon wilt, although These microbial preparations have good effects under laboratory and greenhouse conditions, but there are also limitations in many aspects: First, the single antagonistic bacteria reported in these studies are susceptible to environmental (climate, soil) conditions and their effects are very unstable. Secondly, most of the microbial preparations under development are in liquid form, which is inconvenient in terms of transportation, storage and quality assurance; in addition, the preparation methods and application supporting technologies of microbial preparations have not yet been systematically studied in the existing research. Research has led to high costs and unstable effects for the actual application of microbial technology products to prevent and control watermelon wilt. Therefore, it is still difficult to widely popularize and apply microbial technology to control watermelon wilt

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