Production method of modified chitosan

A production method and technology of chitosan, applied in the field of bioengineering, can solve the problems of high viscosity and low solubility of chitosan, and achieve the effects of wide water solubility, good water solubility and good safety.

Active Publication Date: 2014-12-31
NANCHANG L LIANG IND
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] In order to solve the problems of low solubility of chitosan, precipitation when the pH value is greater than 6.2, and high viscosity, the present invention provides a chitosan with good water solubility, no precipitation at pH 3-10, low viscosity, and retains the original strong adsorption of chitosan. The production method of modified chitosan with functions such as antibacterial and antibacterial properties, the specific technical scheme is as follows:

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] The biosynthesis and application of embodiment 1 chitosan gelatin protein

[0023] Biosynthesis of chitosan gelatin protein: get 100 grams of chitosan, dissolve in hydrochloric acid of 0.1-1.0 M (the same below in mol / L) (or get 100 grams of chitosan hydrochloride, dissolve in water), use 0.1N NaOH or 0.1N NaHCO 3 Adjust the pH value of the solution to 4.0-6.2, add 1-100 grams of gelatin protein (dissolved in water in advance), add 5 grams of TG enzyme (2000 U / g), react at 25°C-85°C for 1-48 hours, and the reaction is over After that, use 0.1N NaOH or 0.1N NaHCO 3 Adjust the pH value of the solution to 6.2-7.0, filter to remove the precipitate, dialysis or ultrafiltration to desalt, spray drying or vacuum drying to obtain chitosan gelatin protein.

Embodiment 2

[0025] Take 100 grams of chitosan, dissolve it in 0.1-1.0 M hydrochloric acid (or take 100 grams of chitosan hydrochloride, dissolve it in water), use 0.1N NaOH or 0.1N NaHCO 3 Adjust to pH 6.2. Add acyl donor gelatin protein, then add 2000 U / g transglutaminase, fully react at 25-85°C, after the reaction, use 0.1N NaOH or 0.1N NaHCO 3 Adjust the pH to 6.2, filter to remove the precipitate, desalt, spray dry or vacuum dry; the mass ratio of chitosan or chitosan inorganic acid salt: gelatin protein: transglutaminase = 1:0.2:0.1.

Embodiment 3

[0026] The biosynthesis and application of embodiment 3 chitosan collagen

[0027] Take 100 grams of chitosan, dissolve it in 0.1-1.0 M hydrochloric acid (or take 100 grams of chitosan hydrochloride, dissolve it in water), use 0.1N NaOH or 0.1N NaHCO 3 Adjust the pH of the solution to 4.0-6.2, add 1-100 grams of collagen (dissolved in water in advance), add 5 grams of TG enzyme, and react at 25°C-85°C for 1-48 hours. After the reaction, use 0.1N NaOH or 0.1N NaHCO 3 Adjust the pH value of the solution to 6.2-7.0, filter to remove the precipitate, dialysis or ultrafiltration to desalt, spray dry or vacuum dry to obtain chitosan collagen.

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PUM

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Abstract

The invention belongs to the field of biological engineering and relates to chitosan modification. The chitosan modification comprises the following steps of: adding protein or polypeptide; adding transglutaminase; adjusting the pH value; removing precipitates after full reaction; and desalting and drying for modification. The modified chitosan produced by the technical scheme disclosed by the invention is good in water solubility, does not generate precipitate at the pH value of 3-10, and is small in viscosity. Moreover, the functions including strong adsorption, antibacterial property and the like of the chitosan are kept, and the application prospect is extensive.

Description

technical field [0001] The invention belongs to the field of bioengineering, in particular to a production method of modified chitosan. technical background [0002] Chitosan (chitosan) is obtained by deacetylation of chitin (chitin), which exists widely in nature, and its chemical name is polyglucosamine (1-4)-2-amino-B-D glucose. Due to the presence of a large number of amino groups in its macromolecular structure and a large number of positive charges, it has a strong adsorption effect on various negatively charged harmful substances. The excellent properties of this natural polymer, such as biofunctionality, compatibility, blood compatibility, safety, and microbial degradability, have attracted widespread attention from all walks of life. It has the functions of controlling cholesterol, inhibiting bacteria, preventing and controlling high blood pressure, adsorption and Physiological functions such as excreting heavy metals, maintaining humidity, and enhancing immunity h...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P19/26
Inventor 刘仁荣朱立鑫
Owner NANCHANG L LIANG IND
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