Biological transformation method for preparing high-activity theasaponin

A technology of biotransformation and tea saponin, applied in biochemical equipment and methods, methods based on microorganisms, microorganisms, etc., can solve the problems of low economic benefits, achieve the effects of reducing pollution emissions, mild experimental conditions, and easy-to-control products

Inactive Publication Date: 2013-08-28
DALIAN POLYTECHNIC UNIVERSITY
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AI Technical Summary

Problems solved by technology

[0004] my country is the country with the highest output of Camellia oleifera in the world, the largest cultivation area and the richest varieties. There are 750,000 tons of Camellia oleifera cake left after oil extraction in the country every year. The utilization rate of domestic resources is only 10%. Due to the bitter taste of Camellia oleifera cake, it cannot be directly As feed, farmers in the producing area often use camellia cake as fertilizer or soil-made pesticides, and the economic benefits are extremely low

Method used

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  • Biological transformation method for preparing high-activity theasaponin
  • Biological transformation method for preparing high-activity theasaponin
  • Biological transformation method for preparing high-activity theasaponin

Examples

Experimental program
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Effect test

Embodiment 1

[0034] (1) Purification of crude tea saponin using AB-8 macroporous resin:

[0035] Soak the crude tea saponin extracted from camellia oleifera cake with ethanol solution with a mass concentration of 70%, filter the clear liquid, steam the ethanol under reduced pressure at 50°C to obtain the clear liquid of camellia oleifera saponin, and put AB-8 macroporous resin on the clear liquid The column was allowed to stand still for full adsorption, washed with deionized water and 0.1% NaOH aqueous solution at a rate of 1.5BV / h in sequence, then washed with deionized water until the pH of the washing solution was 7, eluted with ethanol solution, and the ethanol eluate was collected. Concentrate under reduced pressure at 50°C to dryness to obtain purified tea saponin;

[0036] (2) Preparation of tea saponin enzymatic hydrolysis substrate:

[0037] Put 0.3g of purified tea saponin into a porcelain bowl, add 100mL of methanol to dissolve it, add 3g of 100-200 mesh silica gel to the solu...

Embodiment 2

[0047] The experimental method is the same as in Example 1, except that the bacterial classification used when extracting the enzyme liquid is replaced by Aspergillus niger sp.848 bacteria to obtain 3-oxo-[β-D-galactopyranosyl-(1-2)-β- D-Glucopyranosyl]-21-oxo-angelyl-28-oxo-acetyl-teasapogenin A.

Embodiment 3

[0049] The experimental method is the same as in Example 1, except that the bacterial classification used when extracting the enzyme liquid is replaced by Aspergillus oryzae sp.39 bacteria to obtain 3-oxo-[β-D-galactopyranosyl-(1-2)-β- D-Glucopyranosyl]-21-oxo-angelyl-28-oxo-acetyl-teasapogenin A.

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Abstract

The invention provides a biological transformation method for preparing high-activity theasaponin and belongs to the technical field of biochemical engineering. The biological transformation method comprises the following technological processes of: purifying crude theasaponin by AB-8 macroporous resin, preparing a theasaponin enzymolysis substrate, extracting enzyme liquid, carrying out enzymolysis on the theasaponin and separating enzymolysis components. According to the biological transformation method, firstly sasanguasaponin hydrolytic enzymes are extracted from aspergillus oryzae and aspergillus niger strains, and then sasanguasaponin is hydrolyzed by an enzymic method, experimental conditions are mild, products are easy to control, the pollutant discharge is reduced, and therefore, the biological transformation method is a new green manufacturing technology. Compared with the antioxidant activity of the original sasanguasaponin, the antioxidant activity of the obtained theasaponin is greatly increased. Oil-tea cakes mainly contain crude protein, saccharides, oil, the theasaponin and the like and have very high comprehensive utilization value. Therefore, the oil-tea cakes are used for extracting the theasaponin which is subjected to the enzymolysis, so that the theasaponin with low sugar chain and high activity is obtained. The biological transformation method has prominent economic benefits and social benefits in the research, development and application of the comprehensive utilization of the oil-tea cakes.

Description

technical field [0001] The invention relates to a biotransformation method for preparing highly active tea saponin, which belongs to the technical field of biochemical engineering. Background technique [0002] Tea saponin is a pentacyclic triterpenoid glycoside compound extracted from the Theaceae plant, which widely exists in various tea plants. With the development of modern separation and analysis technology, people have conducted in-depth research on the structure of tea saponin . Due to its special chemical structure, tea saponin has good activity in emulsification, dispersion, moistening, foaming, foam stabilization, decontamination, etc. It is a natural surfactant with excellent performance: widely used in food additives, fungicides . The biological activity of tea saponin is manifested in many aspects such as antibacterial, anti-inflammatory, anti-ulcer, anti-oxidation, and hypolipidemic. [0003] At present, people's research on tea saponins mainly focuses on th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P19/56C12R1/685C12R1/69
Inventor 田晶王秀英费旭徐龙权王一
Owner DALIAN POLYTECHNIC UNIVERSITY
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