Method for detecting hemoglobin by liquid chromatogram-triple tandem quadrupole mass spectrometer

Abstract

The invention discloses a method for detecting hemoglobin by a liquid chromatogram-triple tandem quadrupole mass spectrometer. The method comprises the following steps that 1, a standard substance solution and a sample needing to be detected respectively pass through a solid phase extraction column so that a sample needing to be detected is obtained; 2, the sample needing to be detected is detected by the liquid chromatogram-triple tandem quadrupole mass spectrometer, wherein the chromatographic conditions comprise that a C18 chromatographic column is adopted and a mobile phase A (acetonitrile) and a mobile phase B (formic acid aqueous solution) are used for gradient elution; and 3, through the detection result, hemoglobin content of the detected sample is obtained. Compared with the method recommended by IFCC, the method provided by the invention shortens the whole detection time to 12min, reduces spectrometer operation time by 50%, guarantees good singularity and accuracy, improves a spectrometer utilization rate, and can be used as a reference method for detecting hemoglobin.

Description

technical field [0001] The invention relates to a method for detecting glycosylated hemoglobin by using a liquid chromatography-tandem triple quadrupole mass spectrometer, in particular to a method for detecting the content of glycosylated hemoglobin in a sample to be tested by using a liquid chromatography-tandem triple quadrupole mass spectrometer. Background technique [0002] Glycosylated hemoglobin (abbreviated as hemoglobin A1c, HbA 1c , A1C or HbA1c) is the product of the combination of hemoglobin and glucose in blood red blood cells, which can reflect the average blood sugar level in the patient's body 6-8 weeks before the measurement, and is usually used as a reference standard for monitoring blood sugar control and curative effect observation in diabetic patients. The proportion of hemoglobin being glycosylated is directly proportional to the level of blood glucose concentration in the body over a period of time. Correspondingly, non-glycosylated hemoglobin is rep...

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Problems solved by technology

However, the IFCC recommended detection of HbA 1c method, the experimental operation is more complicated, time-consuming, expensive equipment, maintenance and maintenance costs are high, so far it is still limited to the research on the standardization of glycated hemoglobin

[0013] Due to the current HbA in my country 1c HbA 1c The determination of HbA is not universal, 1c As a diagnostic indicator of diabetes, there are also problems that need to be solved, mainly HbA in my country. 1c The standardization of detection methods is not enough, and the measuring instruments and quality control cannot meet the requirements of current diabetes diagnostic standards. Therefore, the application of HbA is not recommended for the time being. 1c Diagnosis of diabetes, but in order to improve the level of diagnosis, treatment and management of diabetes in my country, in March 2010, China HbA 1c The education program was launched in Beijing. This program is the first large-scale academic activity in China that experts from the endocrinology and laboratory fields jointly participate in, aiming to improve HbA 1c Important role in diabetes management to strengthen clinicians' understanding of HbA 1c awareness and attention

[0014] HbA in my country 1c The measurement standardization work started relatively late and is still in its infancy, but domestic scholars in the testing field are paying more and more attention to the traceability and standardization of the measurement of glycosylated hemoglobin

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