Unlock instant, AI-driven research and patent intelligence for your innovation.

Application of chlorhematin in preparing drug for resisting porcine reproductive and respiratory syndrome virus

A technology for hemin and respiratory syndrome, which is applied to the application field of hemin and the preparation of anti-porcine reproductive and respiratory syndrome virus drugs, can solve the problems that vaccines cannot provide effective and sustainable disease control, and the like, Achieve the effect of mature production process, obvious effect and easy transportation

Inactive Publication Date: 2013-09-11
SUN YAT SEN UNIV
View PDF1 Cites 7 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Porcine reproductive and respiratory syndrome poses a serious threat to the entire swine industry. Due to the lack of understanding of the molecular mechanism of immune evasion of the disease, the current use of vaccines cannot provide effective and sustainable disease control, especially for those RNA-like viruses. At the same time, based on the concern and demand for healthy and safe food, the use of animal antibiotics has caused more and more consumers to oppose

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Application of chlorhematin in preparing drug for resisting porcine reproductive and respiratory syndrome virus
  • Application of chlorhematin in preparing drug for resisting porcine reproductive and respiratory syndrome virus
  • Application of chlorhematin in preparing drug for resisting porcine reproductive and respiratory syndrome virus

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] Example 1 The effect of hemin on the growth and apoptosis of MARC-145 cells

[0032] S1. Cell Viability Assay Experiment

[0033] AlamarBlue is a metabolic indicator for living cells. Under the enzymatic reduction reaction of mitochondria, it will produce measurable fluorescent metabolites, and the growth and viability of cells can be monitored by measuring their fluorescence intensity.

[0034] MARC-145 cells in the logarithmic growth phase were diluted with DMEM medium containing 10% fetal bovine serum and seeded in 96-well plates, 100 μl per well. When the cells were cultured to reach 60-70% confluence, the test drug hemin was added. Set up treatment groups with different concentrations of hemin (the concentrations of hemin were 5 μM, 10 μM, 50 μM, and 100 μM) and a DMSO control group (the concentration of DMSO: 5%), with 6 replicate wells in each group. Set the test time according to the needs of the experiment, replace the culture solution of the cells to be test...

Embodiment 2

[0038] The influence of embodiment 2 hemin on PRRSV viral replication

[0039] S1. Collection of samples

[0040] PRRSV strain CH-1a was inoculated into 80% confluent MARC-145 cells, adsorbed at 4°C and 37°C for 1 h each; the virus liquid was aspirated, and hemin (5 μM, 10 μM, 50 μM) and 2% DMEM maintenance medium with fetal bovine serum; at the same time, DMEM maintenance medium containing 5% DMSO and 2% fetal bovine serum was used as a control, and cells and supernatant were collected at 36 hpi.

[0041] S2. Relative expression of PRRSV N gene in cells detected by qPCR

[0042] Total RNA was extracted using RNAizol reagent from Invitrogen. For adherent cultured MARC-145 cells, remove the culture medium, wash twice with 1×PBS, and then add an appropriate amount of RNAizol to lyse the cells. Transfer the lysate to a 1.5 ml RNase-free EP tube and shake and mix well. After standing at room temperature for 5 minutes, add chloroform according to the ratio of 1ml RNAizol to 200μ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses an application of chlorhematin in preparing a drug for resisting porcine reproductive and respiratory syndrome viruses, and belongs to the technical field of drugs. The Chinese name of Hemin (ChloroprotoporphyrinIXiron (III)) is called the chlorhematin, and the chlorhematin is the oxidized form of ferroheme Heme(ironprotoporphyrinIX). The application disclosed by the invention proves that the chlorhematin plays a role in inhibiting the replication of the porcine reproductive and respiratory syndrome viruses through in-vitro experiments and provides the powerful support for enhancing the prevention and control of the porcine reproductive and respiratory syndrome viruses through drug therapy.

Description

technical field [0001] The invention belongs to the technical field of antiviral research, and in particular relates to the application of hemin in the preparation of anti-porcine reproductive and respiratory syndrome virus drugs. Background technique [0002] Porcine reproductive and respiratory syndrome (PRRS), also known as porcine blue ear disease, is caused by porcine reproductive and respiratory syndrome virus (Porcine reproductive and respiratory syndrome virus, PRRSV), which can cause Pig reproductive disorders (abortion, weak fetus, stillbirth, mummies, etc. in the later stage) and pigs of all ages, especially piglets Respiratory symptoms (interstitial pneumonia) and high mortality are the main infectious diseases of pigs, so it is very important for the pig industry is extremely harmful. [0003] Porcine reproductive and respiratory syndrome poses a serious threat to the entire swine industry. Due to the lack of understanding of the molecular mechanism of immune e...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): A61K31/555A61P31/14
Inventor 刘小红汪亮亮高进涛莫德林陈瑶生
Owner SUN YAT SEN UNIV