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Application of cyclopeptide compound for preventing tumors and preparation method of compound

A technology of cyclic peptide compounds and anti-tumor drugs, which is applied in the field of biomedicine to achieve the effects of high output, increased output, and low cost

Inactive Publication Date: 2013-09-18
XI AN JIAOTONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

After consulting the data, there is no literature report that the cyclic peptide compounds obtained from Streptomyces hawaiiensis NRRL15010 have anti-tumor activity, especially anti-kidney cancer activity

Method used

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  • Application of cyclopeptide compound for preventing tumors and preparation method of compound
  • Application of cyclopeptide compound for preventing tumors and preparation method of compound
  • Application of cyclopeptide compound for preventing tumors and preparation method of compound

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] 1) Streptomyces hawaiiensis NRRL15010 was inoculated into the sterilized initial fermentation broth, and fermented at 30°C with a shaker speed of 300 rpm for 5 to 7 days to obtain the fermentation broth;

[0042] Wherein, the initial culture solution, in terms of mass fraction, comprises: 0.5% D-glucose, 0.5% yeast extract, 0.8% malt extract, 0.05% calcium carbonate, 1% D101 macroporous adsorption resin, and the remaining The amount is water; the Streptomyces hawaiiensis NRRL15010 used in the present invention was purchased from ATCC.

[0043]2) Filter the cultured fermentation broth with 5 layers of gauze to obtain a mixture of filtrate, resin and mycelium, and extract the filtrate three times with ethyl acetate, the volume ratio of ethyl acetate and filtrate used is 1:1; At room temperature, use ethyl acetate and methanol to extract the mixture of resin and mycelium by cold soaking for 3 times, each time for 12 hours, and combine the ethyl acetate extract and the meth...

Embodiment 2

[0058] 1) Streptomyces hawaiiensis NRRL15010 was inoculated into the sterilized initial fermentation broth, and fermented for 5 days at 25°C with a shaker speed of 400 rpm to obtain a fermentation broth;

[0059] Wherein, the initial culture solution, in terms of mass fraction, comprises: 0.3% D-glucose, 0.3% yeast extract, 0.8% malt extract, 0.02% calcium carbonate, 1% D101 macroporous adsorption resin, The balance is water; Streptomyces hawaiiensis NRRL15010 used in the present invention is purchased from ATCC.

[0060] 2) Filter the cultured fermentation bacteria liquid with 5 layers of gauze to obtain a mixture of filtrate, resin and mycelia respectively, and extract the filtrate three times with ethyl acetate, the volume ratio of ethyl acetate and filtrate used is 2:1; At room temperature, use ethyl acetate and methanol to extract the mixture of resin and mycelium by cold soaking for 4 times, each time for 16 hours, and combine the ethyl acetate extract and the methanol e...

Embodiment 3

[0064] 1) Streptomyces hawaiiensis NRRL15010 was inoculated into the sterilized initial fermentation broth, and fermented at 35°C with a shaker speed of 200 rpm for 7 days to obtain a fermentation broth;

[0065] Wherein, the initial culture solution, in terms of mass fraction, comprises: 0.6% D-glucose, 0.6% yeast extract, 0.5% malt extract, 0.02% calcium carbonate, 2% D101 macroporous adsorption resin, and the remaining The amount is water; the Streptomyces hawaiiensis NRRL15010 used in the present invention was purchased from ATCC.

[0066] 2) Filter the cultured fermentation broth with 5 layers of gauze to obtain a mixture of filtrate, resin and mycelia respectively, and extract the filtrate twice with ethyl acetate, the volume ratio of ethyl acetate and filtrate used is 3:1; At room temperature, use ethyl acetate and methanol to extract the mixture of resin and mycelium by cold soaking for 3 times, each time for 24 hours, and combine the ethyl acetate extract and methanol...

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Abstract

The invention discloses an application of a cyclopeptide compound for preventing tumors, and a preparation method of the cyclopeptide compound, belonging to the technical field of biological medicines. The cyclopeptide compound CP-1 or CP-2 has the functions of inhibiting cancer cell growth and proliferation, adjusting and controlling cancer cell periods and adjusting and controlling cancer cell periodic protein expression, so that the cyclopeptide compound can be used for preparing anti-cancer medicaments and / or health care products. The invention further relates to the preparation method of the cyclopeptide compound CP-1 or CP-2. The preparation method comprises the following steps of: with streptomycete as a stain, optimizing the cultivation and fermentation conditions, treating the fermented product, separating the fermented product by using a silica gel column and a reverse phase silica gel column, and finally separating by using a preparative chromatography column so as to obtain the cyclopeptide compound with activity. Compared with the prior art, the preparation method is simple, feasible, environmental-friendly, low in cost and high in yield; and according to the preparation method, the yield of microbial activity secondary metabolite is effectively increased.

Description

technical field [0001] The invention belongs to the technical field of biomedicine, and in particular relates to the anti-tumor application and preparation method of a cyclic peptide compound. Background technique [0002] Cancer is the number one killer of human health. In 2008, the number of cancer cases and deaths in the world was 12.66 million and 7.56 million respectively. It is estimated that there will be 15 million new cases by 2015. The incidence and mortality are increasing year by year. And mainly concentrated in developing countries. [0003] For most cancer patients, there is still a lack of effective therapeutic drugs. Although some of the existing clinically used anti-tumor chemotherapy drugs have certain curative effects, most of them are cytotoxic drugs. , can also inhibit the proliferation of normal cells in the body, such as hair follicle cells and bone marrow cells, and produce certain side effects. The side effects of some chemotherapy drugs are quite l...

Claims

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Application Information

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IPC IPC(8): C07K7/06A61K38/08A61P35/00A23L1/305C12P21/02C07K1/16
Inventor 张海龙郭鹏高阳贺大林
Owner XI AN JIAOTONG UNIV
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