Method for determining content of peimisine in sour pear lung-moistening paste
A determination method, the technology of fritillaria, is applied in the field of determination of the content of fritillaria in Qiuli Runfei Ointment, which can solve the problems of poor reproducibility, low precision, and poor stability of the results, and achieve high recovery and reproducibility. High performance and good control effect
Active Publication Date: 2015-02-04
HEBEI YUZHILIN PHARMA
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Problems solved by technology
[0003] The purpose of the present invention is to solve the technical problems of the existing methods for controlling the quality of Qiuli Runfei Ointment, which are complicated in operation, low in precision, poor in stability and poor in reproducibility of results. In order to solve this problem, we have designed a kind of Qiuli Runfei The method for determining the content of fritillaria in lung ointment is to use high performance liquid chromatography to determine the content of fritillary in Qiuli Runfei ointment. quality
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[0009] A method for assaying the content of fritillaria in Qiuli Runfei Ointment, the key is: the assay method comprises the following steps:
[0010] a. Add analytically pure methanol to the fritillary root reference substance to make a reference substance solution with a fritillary root content of 55-65 μg / ml, and set aside;
[0011] b. Weigh Qiuli Nourishing Lung Ointment, mix it with diatomaceous earth, add analytical pure methanol to reflux extraction, filter, evaporate the filtrate to constant volume, and filter again to obtain the test solution, for subsequent use, wherein Qiuli Nourishing Lung Ointment, The mass fraction ratio of diatomite and analytically pure methanol is 1: (0.8~1.1): (499~501);
[0012] c. Draw equal volumes of the reference substance solution obtained in step a and the test solution obtained in step b, inject them into the high performance liquid chromatograph respectively, measure, calculate by external standard method, and obtain the Fritillaria ...
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Abstract
The invention discloses a method for determining content of peimisine in a sour pear lung-moistening paste, and aims to solve the technical problems of complicated operation, low accuracy, poor stability and poor result repeatability of the traditional method for controlling the quality of the sour pear lung-moistening paste. The adopted technical scheme is that the determination method comprises the following steps of: adding analytical pure methanol to a peimisine reference substance to prepare reference substance solution for future use; weighing the sour pear lung-moistening paste; agitating with kieselguhr; adding the analytical pure methanol to reflux and extract; filtering, evaporating filtrate, fixing volume, and filtering to obtain sample solution for future use; absorbing isometric reference substance solution and sample solution; respectively injecting into an efficient liquid-phase chromatographic instrument; and measuring and calculating according to an external standard method, namely obtaining the content of the peimisine in the sour pear lung-moistening paste. The method has the technical effects of high precision, stability, reproducibility and recovery rate. By using the method, the content of the peimisine in the sour pear lung-moistening paste can be conveniently, rapidly and accurately determined. Thus, the quality of the sour pear lung-moistening paste is well controlled.
Description
technical field [0001] The invention belongs to the technical field of medicine quality control methods, and relates to a determination method, in particular to a determination method for the content of fritillaria in Qiuli Runfei Ointment. Background technique [0002] Qiuli Nourishing Lung Ointment is composed of pear, lily, Chuanbei, Ophiopogon japonicus, and coltsfoot flower. It has the effects of moistening lung and relieving cough, promoting body fluid and relieving throat. It is used for chronic cough, less sticky phlegm, dry mouth and throat. Among them, pear is the monarch drug, Fritillaria and Lily are minister drugs, and its active ingredients are fritillary and colchicine, and the content of fritillary should not be less than 0.60mg / g, but the current standard is only a thin layer of Ophiopogon japonicus. Layer chromatography is used to control the quality of medicines, and there is no content determination item. The thin-layer identification method is to apply a...
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IPC IPC(8): G01N30/02G01N30/06
Inventor 张赞王翠婷霍会玲
Owner HEBEI YUZHILIN PHARMA