Schima superba cold-resistant strain tissue culture seedling twig short ear cutting propagation method

A technology of cutting propagation and tissue culture seedlings, which is applied in the field of asexual propagation of forest tree seedlings, and achieves the effects of high survival rate, increased promotion speed and scale, and high efficiency

Inactive Publication Date: 2013-09-25
CHANGZHOU CITY WUJIN DISTRICT FORESTRY WORKING STATION +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There is no report on the cutting propagation method of using the cold-resistant strain of Schima superba to transplant into live seedlings, twigs and short ears.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0013] 1) Preparation of cutting matrix and root-promoting agent: mix perlite and peat in a ratio of 5:3 (volume ratio) to prepare cutting matrix, spray and sterilize with 3% ferrous sulfate or 0.1% potassium permanganate aqueous solution and set aside; Use indole butyric acid (IBA) 50mg and naphthalene acetic acid (NAA) 150mg to mix in equal amounts, dissolve with a small amount of 95% ethanol and mix with thiamine hydrochloride (VB 1 ) 5mg, add water to make the volume to 1L, make a root promoting agent solution, and set aside;

[0014] 2) During June-October of each year, when the tissue-cultured seedlings of Schima superba cold-resistant strain grow to 50cm high, cut the long 20cm branch, cut the branch into 3cm segments again, keep all the leaves for each short ear cutting, After dipping in the prepared root-promoting agent for 5 seconds, insert it into the prepared matrix;

[0015] 3) Put the cuttings in the plug containing the matrix, and then keep the relative humidit...

Embodiment 2

[0016] 1) Preparation of cutting matrix and root promoting agent: mix perlite and peat in a ratio of 1:1 (volume ratio) to prepare cutting matrix, spray and disinfect with 3% ferrous sulfate or 0.1% potassium permanganate aqueous solution and set aside; Use indole butyric acid (IBA) 150mg and naphthalene acetic acid (NAA) 50mg to mix in equal amounts, dissolve with a small amount of 95% ethanol and mix with thiamine hydrochloride (VB 1 ) 8mg, add water to make the volume to 1L, make a root promoting agent solution, and set aside;

[0017] 2) During June-October of each year, when the tissue-cultured seedlings of Schima superba cold-resistant strain grow to 60cm high, cut the long 30cm branch, cut the branch into 5cm segments again, keep all the leaves for each short ear cutting, After dipping in the prepared root-promoting agent for 5 seconds, insert it into the prepared matrix;

[0018] 3) Put the cuttings in the plug containing the matrix, and then keep the relative humidit...

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PUM

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Abstract

The invention discloses a schima superba cold-resistant strain tissue culture seedling twig short ear cutting propagation method. The method is characterized by including the following steps that 1) cutting media and root growth promoting agents are prepared; 2) when a schima superba cold-resistant strain tissue culture seedling twig grows to be 50-60cm high through breeding, a branch with the length of 20-30cm is cut into small sections with the length of 3-5cm, and all leaves are reserved, soaked in the prepared root growth promoting agents by 2-5s and then inserted into the prepared media; 3) cutting ears are cut in a hole plate with the media, then under the condition full of light and fog, the relative humidity is kept to be 85%-95%, the temperature is kept to be 20-30 DEG C, roots of the cutting ears begin to produce calluses within 12-15 days, root systems grow from the calluses with 20-25 days spent, new buds grow 30 days later, and transplanting is carried out after 45 days. Twig short ears are clipped from tissue culture transplanting survival seedlings to be cut, not only are good characters of original schima superba stock plants kept, but also the cutting survival rate exceeds 92%, operation is simple, cost is low, and efficiency is high.

Description

technical field [0001] The invention relates to a cutting propagation method of plants, in particular to a cuttage propagation method of cold-resistant strains of Schima superbae and transplanted into living seedlings with twigs and short spikes, and belongs to the technical field of asexual propagation of forest tree seedlings. Background technique [0002] Schima superba (Schima superba) belongs to the genus Schima superba of the family Theaceae. It is a large evergreen tree in the subtropical zone. It is widely distributed in 12 southern provinces as a precious high-quality timber and a fast-growing landscape fire-resistant tree species. ①The trunk of the Schima superba is straight, the wood is heavy and dense, the structure is uniform, and the mechanical properties are good. It is a high-quality wood craft species for wooden floors, wooden furniture, wooden toys, and wooden handicrafts; , not strict on soil conditions, strong drought tolerance, dense canopy, thick leathe...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/00A01G31/00
Inventor 潘林蒋泽平李波夏仁俊施燕平卞亚文姜维华张瑛谢玉芳陈建春陈小波
Owner CHANGZHOU CITY WUJIN DISTRICT FORESTRY WORKING STATION
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