Schima superba cold-resistant strain tissue culture seedling twig short ear cutting propagation method
A technology of cutting propagation and tissue culture seedlings, which is applied in the field of asexual propagation of forest tree seedlings, and achieves the effects of high survival rate, increased promotion speed and scale, and high efficiency
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Embodiment 1
[0013] 1) Preparation of cutting matrix and root-promoting agent: mix perlite and peat in a ratio of 5:3 (volume ratio) to prepare cutting matrix, spray and sterilize with 3% ferrous sulfate or 0.1% potassium permanganate aqueous solution and set aside; Use indole butyric acid (IBA) 50mg and naphthalene acetic acid (NAA) 150mg to mix in equal amounts, dissolve with a small amount of 95% ethanol and mix with thiamine hydrochloride (VB 1 ) 5mg, add water to make the volume to 1L, make a root promoting agent solution, and set aside;
[0014] 2) During June-October of each year, when the tissue-cultured seedlings of Schima superba cold-resistant strain grow to 50cm high, cut the long 20cm branch, cut the branch into 3cm segments again, keep all the leaves for each short ear cutting, After dipping in the prepared root-promoting agent for 5 seconds, insert it into the prepared matrix;
[0015] 3) Put the cuttings in the plug containing the matrix, and then keep the relative humidit...
Embodiment 2
[0016] 1) Preparation of cutting matrix and root promoting agent: mix perlite and peat in a ratio of 1:1 (volume ratio) to prepare cutting matrix, spray and disinfect with 3% ferrous sulfate or 0.1% potassium permanganate aqueous solution and set aside; Use indole butyric acid (IBA) 150mg and naphthalene acetic acid (NAA) 50mg to mix in equal amounts, dissolve with a small amount of 95% ethanol and mix with thiamine hydrochloride (VB 1 ) 8mg, add water to make the volume to 1L, make a root promoting agent solution, and set aside;
[0017] 2) During June-October of each year, when the tissue-cultured seedlings of Schima superba cold-resistant strain grow to 60cm high, cut the long 30cm branch, cut the branch into 5cm segments again, keep all the leaves for each short ear cutting, After dipping in the prepared root-promoting agent for 5 seconds, insert it into the prepared matrix;
[0018] 3) Put the cuttings in the plug containing the matrix, and then keep the relative humidit...
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