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Method for preparing compound enzyme and probiotic preparation for feed by biotransformation of pumpkin vine and olive cake

A probiotic preparation and biotransformation technology, applied in animal feed, animal feed, applications, etc., can solve problems such as difficult digestion of monogastric animals, and achieve the effects of low-cost and high-efficiency biotransformation, no waste water discharge, and low energy consumption

Inactive Publication Date: 2014-10-08
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Moreover, while the cake is rich in protein, it also contains a relatively high amount of plant fiber, which is not easy for monogastric animals to digest.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of pumpkin vines, 5kg of olive cake meal, 2kg of wheat bran, 1kg of apple pomace, and 22kg of water, and acclimatize at 35°C After cultivating for 48 hours, a leavening agent is formed;

[0028] 2) Mix 20kg of starter in step 1) with 600kg of pumpkin vines, 300kg of olive cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm. Naturally ventilated and fermented at 40°C After 4 days, a compound enzyme and probiotic preparation for feed was obtained.

[0029] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 42.32IU / g; total cellulase activity reaches 3.88IU / g; beta-glucosidase activity reaches 3.88IU / g; reached 9.73IU / g; endoglucanase activity reached 34.46IU / g...

Embodiment 2

[0031] 1) Fully mix 10kg of fermented Pu-erh tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of pumpkin vines, 5kg of olive cake meal, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and acclimatize at 35°C After cultivating for 48 hours, a leavening agent is formed;

[0032] 2) Mix 20kg of starter in step 1) with 600kg of pumpkin vines, 300kg of olive cake, 60kg of wheat bran, 30kg of apple pomace, and 800kg of water, and use solid-state fermentation with a material thickness of 15cm. Naturally ventilated and fermented at 40°C After 4 days, a compound enzyme and probiotic preparation for feed was obtained.

[0033] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 41.46IU / g; total cellulase activity reaches 3.73IU / g; beta-glucosidase activity reaches 3.73IU / g; reached 9.64IU / g; endoglucanase activity reached 34.46IU / g...

Embodiment 3

[0035] 1) Fully mix 10kg of fermented Pu’er tea, 5kg of shiitake mushroom sticks, 5kg of tea tree mushroom sticks, 10kg of pumpkin vines, 5kg of olive cake meal, 2kg of wheat bran, 1kg of apple pomace, and 25kg of water, and acclimatize at 40°C After culturing for 36 hours, a starter culture was formed;

[0036] 2) Fully mix 10kg of starter in step 1) with 600kg of pumpkin vines, 300kg of olive cake, 60kg of wheat bran, 30kg of apple pomace, and 1000kg of water, and use solid-state fermentation with a material thickness of 10cm, naturally ventilated, and ferment at 35°C for 5 Day, get the compound enzyme and probiotic preparation for feed.

[0037] After determination, the performance indicators of the compound enzyme and probiotic preparation for feed in this embodiment are: carboxymethyl cellulase activity reaches 45.35IU / g; total cellulase activity reaches 4.89IU / g; beta-glucosidase activity reaches 4.89IU / g; reached 10.86IU / g; endoglucanase activity reached 30.28IU / g; xyl...

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Abstract

The invention discloses a method for preparing a composite enzyme / probiotic preparation for feed by bioconverting pumpkin vine and leaf and olive cake pulp, which comprises the following steps: thoroughly mixing Pu'er tea after pile fermentation, shiitake stick, agrocybe aegerita stick, first pumpkin vine, first olive cake pulp, first wheat bran, first apple pomace and first water, and carrying out acclimatization culture to form a leavening agent; and thoroughly mixing the leavening agent, second pumpkin vine, second olive cake pulp, second wheat bran, second apple pomace and second water, and fermenting to obtain the composite enzyme / probiotic preparation for feed. In the invention, the microecologically-stable food-grade safe florae are utilized to ferment the pumpkin vine and olive cake pulp to prepare the composite enzyme / probiotic preparation for feed with favorable performance indexes, thereby implementing low-cost high-efficiency bioconversion of the pumpkin vine and olive cake pulp. Meanwhile, the method disclosed by the invention has the advantages of easy growth of microorganisms, high enzyme activity and extensive fermentation process, and can easily implement industrialized large-scale production.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, and in particular relates to a method for fermenting pumpkin vines and olive cakes with micro-ecologically stable food-grade safe flora to prepare a compound enzyme and probiotic preparation for feed. Background technique [0002] my country is a populous country with extremely tight arable land, and there is a huge gap in the demand for feed raw materials, especially energy feed and protein feed. [0003] Lignocellulose is the most abundant and cheapest renewable resource on earth. The production and processing of agricultural products and food also produces a large amount of lignocellulosic by-products. There are many kinds of lignocellulose, such as various crop straws, urban cellulosic waste, agricultural product processing by-products such as corncobs, vegetable stems and leaves, fruit and vegetable residues, etc. The components of lignocellulose mainly include cellulose, hemicellulose...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A23K1/00A23K1/14
Inventor 阮晖林永华于骅徐薇薇陈功诸文颖
Owner ZHEJIANG UNIV
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