Preparation method and application of fructus podophylli total flavone with multidrug resistance reversing activity
A technology of multi-drug resistance and lobular lotus, which is applied in the field of medicine, can solve the problems that the specific mechanism of tumor inhibition has not been elucidated, and the preparation of lobular lotus total flavonoids has not been seen to reverse the multidrug resistance of leukemia. Operation and method are simple and effective
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Embodiment 1
[0015] 1 kg of lotus lobata, crushed, passed through a 40-mesh sieve, first soaked 4 times with 6 times the weight of petroleum ether, each time for 12 hours, and the residue was soaked 3 times at 50°C with 6 times the weight and volume concentration of ethanol with a concentration of 75%, followed by 12, 8. After 8 hours, the ethanol extracts were combined and concentrated under reduced pressure to a relative density of 2.65g / ml. The concentrated solution of the ethanol extracts was chromatographed on a Sephadex LH-20 gel column, eluted with methanol, and the main spots were collected after a volume concentration of 10 % Sulfuric acid ethanol 105 ° C yellow fraction, concentrated under reduced pressure to a relative density of 1.05g / ml to obtain a concentrated solution, the concentrated solution was spray-dried, and the conditions for spray drying were that the inlet air temperature was 120 °C and the outlet air temperature was 85 °C , atomizer rotating speed is 50 revs / min, f...
Embodiment 2
[0017] 1 kg of lotus lobata, crushed, passed through a 40-mesh sieve, first impregnated with 10 times the weight of petroleum ether for 3 times, each time for 10 hours, and the residue was reflux extracted with 8 times the weight and volume concentration of 95% ethanol for 3 times, followed by 2, 1, 1 hour, combined ethanol extracts, concentrated under reduced pressure to a relative density of 2.75g / ml, the concentrated solution of the ethanol extracts was chromatographed on a Sephadex LH-20 gel column, eluted with methanol, and the main spots were collected through volume concentration 10% ethanol sulfate The yellow fraction at 105°C was concentrated under reduced pressure to a relative density of 1.04g / ml to obtain a concentrated solution, and the concentrated solution was spray-dried. The rotating speed of the device is 55 revs / min, and the feeding speed is 5ml / min to obtain the total flavonoids of Palismanthus microphylla, which is pulverized and passed through a 150-mesh s...
Embodiment 3
[0019] 1 kg of lotus lobata, crushed, passed through a 40-mesh sieve, first soaked twice with 10 times the weight of petroleum ether, each time for 8 hours, and the residue was ultrasonically extracted with 7 times the weight volume concentration of 95% ethanol for 3 times, followed by 30, 20, 20 Minutes, power is 500w, combined ethanol extracts, concentrated under reduced pressure to a relative density of 2.75g / ml, the concentrated solution of the ethanol extracts was subjected to Sephadex LH-20 gel column chromatography, and the main spots were collected through ethanol sulfate with a volume concentration of 10%. The yellow fraction at 105°C was concentrated under reduced pressure to a relative density of 1.02g / ml to obtain a concentrated solution, and the concentrated solution was spray-dried. The spray-drying conditions were that the inlet air temperature was 135°C, the outlet air temperature was 90°C, and the atomization The rotating speed of the device is 55 revs / min, and...
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