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Anti-chicken infectious bursal disease recombinant protein subunit vaccine

A bursal disease and infectious technology, applied in the field of immunology, can solve the problems of virus reversion and inactivation hazards, unsatisfactory effects, etc., and achieve good immunogenicity, good immune protection, and high expression yield

Active Publication Date: 2015-01-21
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This measure can control the occurrence of the disease very well, but for the mutant strains and super-strong strains that appear in some places, because they can break through the protection of maternal antibodies and the immune protection of attenuated live vaccines, the effect of vaccine immunity is not good. Ideal, and they have certain hidden dangers, such as virus reversion and inactivation will bring certain harm

Method used

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  • Anti-chicken infectious bursal disease recombinant protein subunit vaccine
  • Anti-chicken infectious bursal disease recombinant protein subunit vaccine
  • Anti-chicken infectious bursal disease recombinant protein subunit vaccine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1 Fusion expression of infectious bursal disease virus VP2 protein and Salmonella typhimurium flagellin in insect Sf9 cells

[0026] 1.1 Construction of recombinant vector

[0027] 1.1.1 PCR amplification of flagellin+VP2 gene

[0028] Artificially synthesized flagellin+VP2 gene (SEQ ID No.1), flagellin+VP2-F, flagellin+VP2-R are primers, respectively:

[0029] Upstream primer: 5'-GGAATTCTATGGCACAAGTAATCAACAC-3'

[0030] Downstream primer: 5'-CCCAAGCTTTGCTCCTGCAATCTTCAGG-3'.

[0031] After optimizing the PCR reaction conditions and reagents, a 20 μl reaction system was established, as shown in Table 1:

[0032] Table 1

[0033] template

0.2μl

PrimeSTAR

0.2μl

5×PrimeSTAR Buffer

4μl

dNTP Mixture

2μl

flagellin+VP2-F (10pmol)

1μl

flagellin+VP2-R (10pmol)

1μl

Sterile distilled water

11.6μl

[0034] Mix and centrifuge briefly, and place on a PCR instrument for amplification. Re...

Embodiment 2

[0145] Example 2 Study on the immunogenicity of fusion expression product of infectious bursal virus VP2 protein and Salmonella typhimurium flagellin

[0146] 2.1 Materials

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Abstract

The invention provides an anti-chicken infectious bursal disease (IBD) recombinant protein subunit vaccine. The vaccine is a fusion protein having high immunogenicity of Salmonella typhimurium flagellin and an infectious bursal disease virus (VP2). The above flagellin + VP2 fusion protein is obtained through the expression of a recombinant baculovirus containing a flagellin + VP2 gene by utilizing a Bac-to-Bac baculovirus expression system. The recombinant baculovirus obtained through the system has a short period, and the expressed flagellin + VP2 fusion protein has high immune protection force.

Description

technical field [0001] The invention relates to the field of immunology, in particular to a subunit vaccine in which transformed Salmonella typhimurium flagellin (flagellin) + infectious bursal disease virus VP2 fusion protein is an antigen. Background technique [0002] Chicken infectious bursal disease (IBD) is an acute, highly contagious disease caused by infectious bursal disease virus (IBDV). IBDV mainly infects the central immune organ of chicks, the bursa of Fabricius. The disease only causes chickens to be infected and die, and the production performance is reduced. It can also cause severe immunosuppression, resulting in immunosuppression and immunodeficiency. It is one of the main infectious diseases endangering the poultry industry in the world. [0003] In the initial stage of IBDV emergence, the classic strain (cIBDV) was the main epidemic strain, and the incidence rate was high at that time but the mortality rate was not high. Strong virulent strains can caus...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07K19/00C12N15/62A61K39/12A61K39/39A61P31/14C12N15/866
Inventor 刘爵柳舒航韦莉王菁朱珊珊张春燕阎旭全荣
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES