Application of miR-15a as molecular marker for clinical screening of aortic dissection and kit of miR-15a

Abstract

The invention discloses an application of miR-15a as a molecular marker for clinical screening of aortic dissection, wherein the sequence of miR-15a is showed as the SEQ ID NO.1. A kit for clinical screening of aortic dissection is characterized by comprising: a blood plasma general RNA extraction reagent group, an exogenous reference object, a RNA reverse transcription reagent group, a RNA reverse transcription primer group, a realtime quantitative PCR reagent group, a realtime quantitative PCR primer group and a realtime quantitative PCR probe group, wherein the RNA reverse transcription primer group comprises a miR-15a reverse transcription primer and an exogenous reference object reverse transcription primer, the realtime quantitative PCR primer group comprises a miR-15a realtime quantitative PCR primer and an exogenous reference object realtime quantitative PCR primer, and the realtime quantitative PCR probe group comprises a miR-15a realtime quantitative PCR probe and an exogenous reference object realtime quantitative PCR probe.

Description

technical field [0001] The invention relates to the technical field of biological detection. Background technique [0002] Aortic dissection refers to the high-speed and high-pressure blood flow in the aorta entering the media layer or the junction of the media and adventitia through the intimal tear on the aortic wall, thereby forming a true and false aortic cavity, and expanding along the long axis of the aorta , leading to aortic rupture leading to death of the patient or acute ischemia of important organs (such as: heart, brain, kidney, intestinal tract, etc.) causing serious complications, the condition is dangerous, and it is one of the most serious diseases involving the aorta; according to the course of the disease, it can be Divided into acute aortic dissection (acute aortic dissection, AAD), subacute aortic dissection (subacute aortic dissection, SAD) and chronic aortic dissection. In our clinical practice, we found that many patients with aortic dissection died o...

AI Technical Summary

Problems solved by technology

The academic community has done a lot of research to find biomarkers of aortic dissection, and found that such as: C-reactive protein, D-dimer, smooth muscle myosin heavy chain, calponin, etc. may be related to the occurrence of acute aortic dissection. However, due to factors such as lack of specificity, short half-life, or limitation of false lumen thrombosis, they have not been widely used in the clinical diagnosis and treatment of acute aortic dissection

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