Application of miR-15a as molecular marker for clinical screening of aortic dissection and kit of miR-15a

1. The technology of mir-15a and aortic dissection is applied in the direction of microbial measurement/inspection, biochemical equipment and methods, etc., which can solve the problems of short half-life and lack of specificity, and achieve strong specificity, high sensitivity, and good The effect of clinical application prospects

Active Publication Date: 2013-10-23
SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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The academic community has done a lot of research to find biomarkers of aortic dissection, and found that such as: C-reactive protein, D-dimer, smooth muscle myosin heavy chain, calponin, etc. may be related to the occurrence of...

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  • Application of miR-15a as molecular marker for clinical screening of aortic dissection and kit of miR-15a
  • Application of miR-15a as molecular marker for clinical screening of aortic dissection and kit of miR-15a
  • Application of miR-15a as molecular marker for clinical screening of aortic dissection and kit of miR-15a

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Embodiment Construction

[0016] The specific embodiments of the present invention will be described in detail below in conjunction with the accompanying drawings.

[0017] In the specific implementation process of the present invention, the reagents used in the corresponding steps are as follows: RNA extraction is carried out with the miRNeasy Mini Kit purchased from Qiagen, which includes 2ml centrifuge tube (without cover), 1.5ml centrifuge tube (with cover), QIAzol lysis Reagent, RNA / DNase-free water, RWT buffer, RPE buffer, and Rneasy Mini spin column filter column; purchased from Applied Biosystems MicroRNA Reverse Transcription Kit (Catalog No. 4366597) for RNA reverse transcription, including: 10×Reverse Transcription Buffer, 100mM dNTPs(with dTTP), 20U / μl Rnase Inhibitor, 50U / μl MultiScribeTM Reverse Transcriptase; purchased from Applied Biosystems Universal PCR Master Mix II, no UNG (Cat. No. 4440048) for real-time quantitative PCR. In addition, RNA reverse transcription primers and ...

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Abstract

The invention discloses an application of miR-15a as a molecular marker for clinical screening of aortic dissection, wherein the sequence of miR-15a is showed as the SEQ ID NO.1. A kit for clinical screening of aortic dissection is characterized by comprising: a blood plasma general RNA extraction reagent group, an exogenous reference object, a RNA reverse transcription reagent group, a RNA reverse transcription primer group, a realtime quantitative PCR reagent group, a realtime quantitative PCR primer group and a realtime quantitative PCR probe group, wherein the RNA reverse transcription primer group comprises a miR-15a reverse transcription primer and an exogenous reference object reverse transcription primer, the realtime quantitative PCR primer group comprises a miR-15a realtime quantitative PCR primer and an exogenous reference object realtime quantitative PCR primer, and the realtime quantitative PCR probe group comprises a miR-15a realtime quantitative PCR probe and an exogenous reference object realtime quantitative PCR probe.

Description

technical field [0001] The invention relates to the technical field of biological detection. Background technique [0002] Aortic dissection refers to the high-speed and high-pressure blood flow in the aorta entering the media layer or the junction of the media and adventitia through the intimal tear on the aortic wall, thereby forming a true and false aortic cavity, and expanding along the long axis of the aorta , leading to aortic rupture leading to death of the patient or acute ischemia of important organs (such as: heart, brain, kidney, intestinal tract, etc.) causing serious complications, the condition is dangerous, and it is one of the most serious diseases involving the aorta; according to the course of the disease, it can be Divided into acute aortic dissection (acute aortic dissection, AAD), subacute aortic dissection (subacute aortic dissection, SAD) and chronic aortic dissection. In our clinical practice, we found that many patients with aortic dissection died o...

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Application Information

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IPC IPC(8): C12Q1/68
Inventor 周建景在平陆清声董健赵志青包俊敏冯睿冯翔袁良喜
Owner SECOND MILITARY MEDICAL UNIV OF THE PEOPLES LIBERATION ARMY
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