Method of visually determining site of origin of urine odor formation

A determination method, technology of the production department, applied to the measurement/inspection of microorganisms, biochemical equipment and methods, analysis by causing chemical reactions of materials, etc.

Active Publication Date: 2013-11-13
KAO CORP
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For example, in a toilet, urine remaining on the outside of the toilet bowl as droplets is difficult to visually confirm its existence, so it remains there for a long time, and may become a source of bad odor

Method used

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  • Method of visually determining site of origin of urine odor formation
  • Method of visually determining site of origin of urine odor formation
  • Method of visually determining site of origin of urine odor formation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0122] Example 1 Selection of Enzyme Activity Detection Reagent (Component A)

[0123] Such as figure 1 As indicated, 0.03 g of water-absorbent polymer was absorbed into 300 μL of each solution containing an enzyme activity detection reagent or an odor chromogenic indicator of the composition shown in Table 1, and it was further sandwiched in a 1.5 mL capacity micro-test tube. Pulp layer and laminated into 3 layers. Enzyme or bacteria as an initiator of odor generation was added only to the central layer, and odor generation was performed for 72 hours in a thermostatic bath at 37° C., and the color change was observed. The results are shown in Table 2 and figure 2 middle. Table 2 shows the results of chromogenicity, contrast with urine color, and accuracy of identification of urine odor generating parts determined by 4 panelists in accordance with the following criteria according to the agreement.

[0124]

[0125] a: strong hair color

[0126] b: slightly intense colo...

Embodiment 2

[0146] Example 2 Visualization of urine odor generating parts and intensity of urine odor, and screening of water-absorbent polymers

[0147] (1) Collection of β-glucuronidase active strains

[0148] SCDLP agar plate medium (manufactured by Nippon Pharmaceutical Co., Ltd.) supplemented with 5-bromo-4-chloro-3-indole-β-D-glucuronide was used from baby diapers after use (only urination). The collection and isolation of β-glucuronidase active strains were carried out according to common methods. These strains were cultured in sterilized urine, and the generation of odor was confirmed, and the strain which produced especially strong odor among them was used as the β-glucuronidase active strain of this Example. In addition, the strain was confirmed to belong to Escherichia coli by 16S rDNA partial base sequence analysis.

[0149] (2) Preparation of bacterial solution

[0150] Cultivate the above-mentioned β-glucuronidase active strains on SCD agar plate medium for one night, tak...

Embodiment 3

[0165] Example 3 Screening of Antimicrobial Agents for Urine Odor Generation Inhibition

[0166] The organic antimicrobial agents P and Q or the inorganic antibacterial agent R were added at various concentrations (w / w %) to the water-absorbing polymer as a urine odor generation inhibitor. In addition, a quaternary ammonium salt-based antibacterial agent was used as the organic antibacterial agent P, a phenolic antibacterial agent was used as the organic antibacterial agent Q, and zinc sulfate was used as the inorganic antibacterial agent R. 0.45 g each of the obtained antimicrobial agent-added water-absorbing polymer was evenly arranged on the bottom surface of a rectangular container with a bottom surface of approximately 5.5 cm, and 6.5 mL of the liquid preparation prepared in (3) of Example 2 was added thereto, and kept at a constant temperature of 30°C The enzyme reaction was carried out in the tank for 48 hours. Figure 6 The image of each sample is shown in the center ...

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Abstract

A method of visually determining a site of origin of urine odor formation and the intensity of urine odor, wherein the method comprises a first step of applying a liquid preparation containing a component (A) to an object, facility or material to be determined on urine odor, a second step of allowing an enzymatic reaction to proceed in the object, facility or material to which the liquid preparation has been applied, and a third step of observing the change in color in the object, facility or material to which the liquid preparation has been applied, wherein a region in which the color is changed is determined as a site of origin of urine odor formation and the amount of change in color is assigned to the intensity of urine odor; and a method of screening for a material, a member or a urine odor formation inhibitor each having an excellent urine odor formation inhibitory effect, or screening for a way of placing the member, wherein the method comprises bringing a liquid preparation containing the component (A) into contact with each of materials, members or agents which are subjects to be evaluated on a urine odor formation inhibitory effect, and selecting a subject that shows little change in color through an enzymatic reaction among from the above-mentioned subjects. The component (A): an enzyme activity detection reagent which can act as a substrate for an enzyme and can produce a poorly-water-soluble pigment compound or a poorly-water-soluble fluorescent pigment compound through an enzymatic reaction.

Description

technical field [0001] The present invention relates to a urine odor generating part and a simple determination method of urine odor intensity, and further relates to a screening method for an absorbent article, a water-absorbing polymer, and a urine odor generation inhibitor having an excellent urine odor generation suppression effect using the method. Background technique [0002] In recent years, due to the improvement of consumers' awareness of hygiene, it is strongly desired to remove not only external dirt but also odor associated with the presence of dirt. In particular, the odor derived from urine is particularly unpleasant to a person in the malodor of the living environment. For example, in a toilet, since it is difficult to visually confirm the presence of urine that remains outside the toilet bowl as droplets, it remains there for a long time and may become a source of bad odor. In addition, hygiene products such as underwear, diapers, and sanitary products may ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/40C12Q1/44G01N21/78
CPCG01N21/6428C12Q1/34G01N21/78C12Q1/44
Inventor 森一郎石田浩彦前田胜司
Owner KAO CORP
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