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OX26/CTX-PL/pC27 compound and application of OX26/CTX-PL/pC27 compound in treating gliomas

A glioma, OX26 technology, applied in gene therapy, genetic material components, medical preparations with non-active ingredients, etc. Compatibility, convenient clinical application effect

Active Publication Date: 2013-11-27
SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Furthermore, more nano-carriers tend to target the brain of chemotherapy drugs, such as doxorubicin, paclitaxel and other traditional drugs, and the research on targeting nano-gene carriers is rare.

Method used

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  • OX26/CTX-PL/pC27 compound and application of OX26/CTX-PL/pC27 compound in treating gliomas
  • OX26/CTX-PL/pC27 compound and application of OX26/CTX-PL/pC27 compound in treating gliomas
  • OX26/CTX-PL/pC27 compound and application of OX26/CTX-PL/pC27 compound in treating gliomas

Examples

Experimental program
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Effect test

Embodiment 1

[0039] The construction of embodiment 1 double targeting gene carrier complex

[0040] 1) Research on the preparation process of liposome complexes

[0041] The liposome complexes loaded with pC27 were prepared by the reverse-phase evaporation method, and the temperature during rotary evaporation (20°C, 30°C and 40°C), ultrasonic temperature (4°C, 10°C and 20°C), ultrasonic time (2min , 5min and 10min), solvent types (ether, ethyl acetate and chloroform) and other formulation process factors on the particle size of cationic liposomes. Results (see figure 1 , 2) It shows that the temperature during rotary evaporation has little effect on the particle size of liposomes, but too high a temperature will easily cause the solution to bubble during the rotary evaporation process, which will affect the quality of the coating, so the temperature of the rotary evaporation is selected as 30°C; When the temperature is 4°C, the oil-water two phases cannot be fully mixed, and better lipos...

Embodiment 2O

[0064] Example 2 Evaluation of the therapeutic effect of OX26 / CTX-PL / pC27 on rats bearing glioma

[0065] Grouping of experimental animals: Animals were randomly divided into 5 groups, 15 in each group: (1) PBS control group; (2) OX26 / CTX-PL / pEGFP treatment group; (3) PL / pC27 treatment group; (4) OX26 -PL / pC27 treatment group and (5) OX26 / CTX-PL / pC27 treatment group.

[0066] Establishment of orthotopic glioma model in rats: Wistar rats were anesthetized by intraperitoneal injection of 10% chloral hydrate (4ml / kg), and then the heads of the rats were fixed on a brain stereotaxic apparatus. Determine the right caudate nucleus as the target according to the stereotaxic anatomy map of the rat head, 1 mm behind the coronal suture and 3 mm to the right side of the midline, drill a small hole with a dental drill, and then under the guidance of a stereotaxic instrument, inject a 50 μl microinjector Draw 10 μl of C6 cell suspension (1×10 6 cells), slowly insert the needle vertically...

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Abstract

The invention discloses an OX26 / CTX-PL / pC27 compound and the application of the OX26 / CTX-PL / pC27 compound in treating gliomas. The full name of the OX26 / CTX-PL / pC27 compound provided by the invention is OX26 / CTX coupled PEG-lated liposome pC27 compound; the OX26 / CTX-PL / pC27 compound is a nano-gene vector which takes the receptor antibody OX26 and CTX of transferrin as targeted modification ligands, takes hTERTC27 as a therapeutic gene of the glioma, and takes the PEG lipidosome as a vector skeleton. The vector can be used for preparing drugs for treating gliomas, and can penetrate blood brain barriers, meanwhile, has a targeting effect on the gliomas, and can treat the gliomas effectively and safely.

Description

technical field [0001] The invention relates to the field of nano-gene carriers, in particular to a nano-gene carrier OX26 / CTX-PL / pC27 complex capable of passing through the blood-brain barrier and targeting glioma, and its construction method and application. Background technique [0002] Glioma is the most common primary intracranial tumor, with an annual incidence of 6 / 100,000. Although great progress has been made in glioma surgery, chemotherapy and radiotherapy in the past 30 years, the median survival time of patients with malignant glioma is only 14.6 months. Therefore, the clinical treatment of glioma is still a difficult problem that needs to be studied and explored. [0003] An important reason for the failure of glioma treatment is that it is difficult for antitumor drugs to reach the brain parenchyma through the blood-brain barrier. However, traditional gene carriers, including nanocarriers, do not have the ability to penetrate the blood-brain barrier, which se...

Claims

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Application Information

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IPC IPC(8): A61K48/00A61K47/42A61K47/34A61K9/127A61P35/00
Inventor 彭英
Owner SUN YAT SEN MEMORIAL HOSPITAL SUN YAT SEN UNIV
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