Unlock instant, AI-driven research and patent intelligence for your innovation.

Glutamic acid decarboxylase extracted from grapes and extraction method as well as Gamma-aminobutyric acid production method

A technology of glutamic acid decarboxylase and grape, which is applied in the direction of lyase and fermentation, can solve the problems of low enzyme activity and long extraction time, and achieve the effects of simple process, reduced production cost and short time consumption

Active Publication Date: 2013-11-27
GUANGZHOU WEIRUTANG NUTRITION & HEALTH CONSULTING CO LTD
View PDF2 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0006] The purpose of this invention is to provide a kind of glutamic acid decarboxylase extracted from grapes and the method for extracting and producing gamma-aminobutyric acid, solve the technical problem that extraction time is longer, enzyme activity is lower in the prior art, simultaneously Provide a new method for grape deep processing and increase added value; and reduce the production cost of GABA, and ensure the food safety of GABA products

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0030] Select fresh non-rotten grapes, wash and remove the soil, use a soymilk machine to crush until muddy, the fiber length is about 1mm, filter the residue and put it in a triangular flask, add 1 times the phosphate buffer solution of pH 5.5, extract for 0.5h, filter , mixed the juice twice, added chitosan 0.1% to the grape juice, after mixing evenly, let it stand for 30 minutes, and centrifuged at 10,000rpm for 15 minutes to obtain the GAD enzyme solution. The enzyme activity of the crude enzyme solution detected by colorimetry was 9.0U.

Embodiment 2

[0032] Select fresh non-rotten grapes, wash and remove the soil, use a soymilk machine to crush until muddy, the fiber length is about 2mm, filter the residue and put it in a triangular flask, add 3 times the phosphoric acid buffer solution of pH 5.5, and extract for 0.5h. Filter and mix the juice twice, add 0.1% chitosan to the grape juice, mix well, let it stand for 30 minutes, and centrifuge at 10,000rpm for 15 minutes to obtain GAD enzyme solution. The enzyme activity of the crude enzyme solution detected by colorimetry is 6.9U.

Embodiment 3

[0034] Select fresh non-rotten grapes, wash and remove the soil, use a soymilk machine to crush until muddy, the fiber length is about 1mm, filter the residue and put it in a triangular flask, add 1 times the phosphate buffer solution of pH 5.5, extract for 1 hour, and filter , mix the juice twice, add 0.1% chitosan to the grape juice (that is, the mass volume ratio of chitosan to grape juice is 0.1%, the unit is g / ml), mix well, let stand for 30min, and centrifuge at 10000rpm for 15min , to obtain GAD enzyme solution, and the enzyme activity of the crude enzyme solution detected by colorimetry was 9.9U.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for extracting glutamic acid decarboxylase from grapes. The method comprises the following steps: selecting fresh no-rotten grapes, and cleaning the grapes to remove soil; mashing the grapes, so that the fiber length of the grapes is no more than 2 mm; taking residues out; adding a phosphoric acid buffered solution with Ph of 5.5, wherein the mass of the phosphoric acid buffered solution is 1 to 3 times of that of the residues; carrying out digestion on the grapes for 0.5 to 1 hour, filtering, and merging secondary juice; adding chitosan into the grape juice, wherein the ratio of mass to volume of the chitosan is 0.05 % to 0.12%, placing the grape juice statically for 30 minutes after mixed uniformly; centrifuging the grape juice for 10 to 15 minutes at 7,000 to 10,000 rpm so as to obtain GAD enzyme liquid. The invention further provides the glutamic acid decarboxylase extracted from grapes and Gamma-aminobutyric acid production method by adopting the glutamic acid decarboxylase. According to the invention, the enzyme liquid extracted from the grapes has higher activity, which can reach 9.5 U, and can be adopted to produce GABA, and the percent conversion of glutamic acid can reach 95%.

Description

technical field [0001] The invention belongs to the field of production technology of food functional components, and relates to a glutamic acid decarboxylase extracted from grapes and a method for extracting it. The present invention also relates to a method for producing γ-aminobutyric acid. Background technique [0002] γ-aminobutyric acid is a non-protein functional amino acid that widely exists in nature and is an important inhibitory neurotransmitter in the central nervous system of mammals. It has important body functions, such as regulating blood pressure and heart rate, nutrition Nerve cells, promote mental stability, promote blood flow in the brain, enhance brain vitality and other functions. [0003] The content of GABA in natural food materials is very small. The content of GABA in higher plants is usually 0.13-32.15umol / g. It can be as high as 6 times that of ordinary brown rice, which is far from enough for people who need to improve their health. For exampl...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): C12N9/88C12P13/00
Inventor 庄义庆吴琴燕杨敬辉
Owner GUANGZHOU WEIRUTANG NUTRITION & HEALTH CONSULTING CO LTD