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Preparation method of sea anemone low molecular polypeptides

A low-molecular and sea anemone technology, applied in the field of extraction and preparation of sea anemone low-molecular-weight polypeptides, can solve problems such as non-conformity with the mainstream, a large amount of fresh water and energy, and reduce production costs, improve extraction rate and purity, and reduce production energy consumption. Effect

Inactive Publication Date: 2013-12-04
NINGBO BOFENG BIOTECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The hot water extraction method needs to use a large amount of fresh water and energy, which is not in line with the mainstream of national and world economic development

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0008] Example 1: After soaking and cleaning the raw material sea anemone, first use a pulverizer to pulverize it into a fluid slurry, then enter an industrial-grade colloid mill for ultrafine pulverization to a particle size of about 30 microns, and mix it with 8 times the volume of water evenly Then enter the high-speed centrifuge at 12,000 rpm for 30 minutes to remove non-collagenous water-soluble substances, and the collagen-containing precipitate and 3 times the volume of water enter the biological enzymatic hydrolysis tank for compound enzymatic hydrolysis treatment. The composite enzyme is mainly composed of trypsin, bromelain and collagenase, the added amount is 0.1% of the raw material, the reaction conditions are temperature 37°C, pH 8.0, and reaction time 2 hours. The enzymatic hydrolysis product is treated with ultrafiltration equipment. The permeation molecular weight of the ultrafiltration membrane is 3KD. Those with a molecular weight less than 3KD pass through t...

example 2

[0009] Example 2: After soaking and cleaning the raw material sea anemone, first use a pulverizer to pulverize it into a fluid slurry, then enter an industrial-grade colloid mill for ultrafine pulverization to a particle size of about 50 microns, and mix with 2 times the volume of water evenly Then enter the high-speed centrifuge at 8000 rpm for continuous centrifugation for 30 minutes to remove non-collagen water-soluble substances, and the collagen-containing precipitate and 5 times the volume of water enter the biological enzymatic hydrolysis tank for compound enzymatic hydrolysis treatment. The compound enzyme is mainly composed of trypsin, bromelain and collagenase, the added amount is 3% of the raw material, the reaction conditions are temperature 65°C, pH 7.5, and reaction time 8 hours. The enzymatic hydrolysis product is treated with ultrafiltration equipment. The permeation molecular weight of the ultrafiltration membrane is 3KD. Those with a molecular weight less than...

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Abstract

The invention discloses a preparation method of sea anemone low molecular polypeptides. The preparation method comprises the following steps: immersing and cleaning sea anemones, crushing the sea anemones by using a crusher, adding to an industrial grade colloid mill, carrying out ultramicro crushing to a particle size of 30-50mum, uniformly mixing with water having a volume 2-8 times the volume of obtained particles, adding to a centrifuge, continuously centrifuging at 8000-12000rpm for 30min, removing the obtained supernatant, adding the obtained precipitate and water having a volume 3-5 times the volume of the obtained precipitate to a biological enzymatic hydrolysis pot, carrying out enzymatic hydrolysis under conditions comprising a temperature of 37-65DEG C and a pH value of 7.5-8.0 for 2-8h, processing the obtained enzymatic hydrolysis product by a 3KD ultrafilter membrane, carrying out nanofiltration membrane concentration of the part having a molecular weight below 3KD in the product, and carrying out spray drying to obtain the finally-finished sea anemone low molecular polypeptides, wherein the addition amount of a composite enzyme in the above enzymatic hydrolysis reaction is 0.1-3% of the amount of a raw material. The part having a molecular weight above 3KD in the enzymatic hydrolysis product returns to the biological enzymatic hydrolysis pot for enzymatic hydrolysis.

Description

technical field [0001] The invention belongs to a method for extracting seafood, in particular to a method for extracting and preparing sea anemone low-molecular-weight polypeptides. technical background [0002] Sea anemones, also known as sea chrysanthemums and Haidian roots, belong to the phylum Coelenterata, the class Anthozoa, and the subclass Hexacorallia. There are 3 orders under it, including Actiniaria, Zoanthidea and Ceriantharia. There are many kinds of sea anemones, more than 1,000 species have been reported, and they are widely distributed in various sea areas of the world. The species of sea anemones in China account for about 1 / 10 of the world's total. The tentacles and body of sea anemones contain a large number of nematocysts, each of which has a special sac-shaped organelle called nematocyst, which stores venom, and the nematocyst is coiled in the nematocyst. The tip is a needle. When the sea anemone is stimulated by external machinery or chemicals, it w...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06C07K1/34
Inventor 张建芳
Owner NINGBO BOFENG BIOTECH
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