Preparation method and application of crocodile-skin collagen peptide
A technology of skin collagen and protein peptides, which is applied in skin care preparations, animal/human proteins, specific peptides, etc., can solve the problems of lack of secondary utilization of leftovers, incomplete development, environmental pollution, etc., and achieve good social benefits and Economic benefits, increase added value, and reduce environmental pollution
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Embodiment 1
[0043] Take 100g of fresh crocodile skin, scrape off the fat, wash it, cut it into square pieces with a side length of about 2 cm, add 1L of distilled water, cook in an electric rice cooker for 30 minutes, cool to room temperature, and mash. Add 4% alkaline protease (alkaline protease derived from Bacillus licheniformis, Zaozhuang Jienuo Biological Enzyme Co., Ltd.) based on the weight of crocodile skin, after hydrolysis at pH 11, temperature 40 ° C for 3 hours, heat and boil for 10 minutes to make the enzyme Inactivate. Centrifuge at room temperature at 4000 rpm for 20 minutes, take the supernatant, and freeze-dry to obtain crocodile skin collagen peptide powder.
[0044] Figure 4 The MALDI-TOF MS spectrum of the crocodile skin collagen peptide obtained in Example 1 is shown. The molecular weight of the crocodile skin collagen peptide powder that embodiment 1 obtains is 1.2KD. The molecular weight is small, indicating that the enzymatic hydrolysis is sufficient during the...
Embodiment 2
[0046] Take 500g of fresh crocodile skin, scrape off the fat, wash it, cut it into small pieces, add 5L of distilled water, cook in an electric rice cooker for 30 minutes, cool to room temperature, and mash. Add 4% alkaline protease (alkaline protease derived from Bacillus licheniformis, Zaozhuang Jienuo Biological Enzyme Co., Ltd.) based on the weight of crocodile skin, after hydrolysis at pH 11, temperature 40 ° C for 3 hours, heat and boil for 10 minutes to make the enzyme Inactivate. Then centrifuge at 4000 rpm for 20 minutes at room temperature, take the supernatant, and spray dry to obtain crocodile skin collagen peptide powder.
[0047] The molecular weight of the obtained crocodile skin collagen peptide powder is 1.2KD. The molecular weight is small, indicating that the enzymatic hydrolysis is sufficient during the treatment with alkaline protease. The content of hydroxyproline was measured to be 6.2 μg / mg. The aqueous solution of crocodile skin collagen peptide pow...
Embodiment 3
[0048] Embodiment 3: the in vitro moisturizing effect of the crocodile skin collagen peptide of the present application
[0049] According to the difference in moisturizing properties of humectants, different humectants have different forces on water molecules, and the ability to absorb water and retain water is also different. Dry the samples containing a certain amount of moisture in a desiccator with constant temperature and humidity, and regularly weigh the reduction of the sample mass. Through comparative analysis, the moisture retention of different samples can be compared. In order to better simulate the actual situation of the skin, affix 3M adhesive plaster to the slide, and each test substance (respectively distilled water (negative control), 0.3% hyaluronic acid (positive control), crocodile prepared in Example 1) Skin collagen peptide and sharkskin collagen peptide) aqueous solution (solid-liquid ratio is 1: 4) is coated on it, puts in the desiccator that fills s...
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