A method for the detection of trace amounts of ochratoxin a by electrochemical aptasensor
An aptamer sensor and ochratoxin technology, which can be applied to measurement devices, scientific instruments, instruments, etc., can solve the problem that electrical signal changes are not very obvious, and achieve the effects of low detection cost, fast analysis speed, and simplified modification process.
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Embodiment 1
[0028] Example 1: Preparation of aptamer sensor
[0029] (1) Preparation of DNA3-GNPs: take 100 mL of 0.01% HAuCl 4 Heat to boiling under stirring, then add 10 mL of 1% sodium citrate, continue to heat and stir to react for half an hour, then stop heating, stir and cool to room temperature to obtain gold glue, and its transmission electron microscope image is shown in figure 1 middle. Take 2 mL of gold gel and add 30 μL of 100 μM DNA3 solution to incubate for 12 h, then add 0.2 mL of 2 M NaCl solution, and continue to incubate for 24 h. After the reaction was completed, the obtained solution was washed twice with water, centrifuged at 12,000 r / min for 10 min, and repeated three times to prepare DNA3-GNPs. Finally re-dispersed in 600 μL Tris-HCl (pH 8.0) for use.
[0030] (2) Gold electrode pretreatment: First, the gold electrode was polished with 1 μm, 0.5 μm, and 0.05 μm alumina powder in turn, and K 3 Fe(CN) 6 As a probe scanning peak potential difference is less tha...
Embodiment 2
[0032] Example 2: Drawing the standard curve of the aptamer sensor to the trace OTA standard sample
[0033] The aptamer sensor was first placed in the OTA standard solution with concentrations of 0, 0.001, 0.01, 0.1, 0.5, 1, 2, and 4 ng / mL, respectively, for 15 min, and then rinsed three times with secondary water. Then soak in 80 μM MB solution for 10 min. After washing with water twice, the aptamer sensor was used as the working electrode, the platinum wire was used as the counter electrode, and the saturated calomel electrode was used as the reference electrode. Collect the corresponding MB reduction peak current signal, draw a standard curve, and the results are shown in image 3 middle.
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