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Preparation method of stasis-phlegm type ovarian cyst animal model

A technology for phlegm-stasis intertwined type and ovarian cysts, applied to preparations for in vivo experiments, medical raw materials derived from mammals, medical preparations containing active ingredients, etc., can solve difficult new drug screening, no phlegm-stasis interaction Nodular ovarian cyst and other problems, to achieve the effect of strong reproducibility and simple administration method

Inactive Publication Date: 2013-12-11
CHENGDU MEDICAL COLLEGE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0004] At present, there is no animal model of ovarian cyst with phlegm and stasis, and it is difficult to effectively screen new drugs

Method used

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  • Preparation method of stasis-phlegm type ovarian cyst animal model
  • Preparation method of stasis-phlegm type ovarian cyst animal model
  • Preparation method of stasis-phlegm type ovarian cyst animal model

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] Embodiment 1 The establishment method of the animal model of phlegm and blood stasis intermingled ovarian cyst of the present invention

[0025] 1 Materials and methods

[0026] 1.1 Experimental animals

[0027] Healthy SD clean female non-pregnant rats, weighing 150±10g. Provided by the Experimental Animal Center of Sichuan University (animal qualification certificate number: SCXK Chuan 2008-24), it will be used after 1 week of adaptive feeding.

[0028] 1.2 Main reagents

[0029] Estradiol Benzoate Injection, the concentration of Estradiol Benzoate is 5mg / ml (Tianjin Jinyao Amino Acid Co., Ltd., National Drug Approval H12020529); Olive Oil (Chengdu Kelong Chemical Preparation Factory); Cholesterol (Shanghai Boao Biotechnology Co., Ltd.); Medicinal Tween-80 (Nanjing Well Chemical Co., Ltd.); Rapidly Improved Papanicolaou Staining Solution (Nanjing Jiancheng Technology Co., Ltd.); Estradiol EISA Kit (Shanghai Jingyan Biotechnology Co., Ltd.) ;Total cholesterol (CHO)...

Embodiment 2

[0068] Embodiment 2 uses the model of the present invention to screen the medicine for the treatment of phlegm and stasis intermingled ovarian cyst

[0069] a, the phlegm and stasis intermingled ovarian cyst established according to the method of Example 1;

[0070] b. administering the drug candidate to an animal model;

[0071] c. Observe the effect of candidate drugs on various indicators of ovarian cysts with phlegm and stasis, and evaluate potential drugs for treating ovarian cysts with phlegm and stasis.

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Abstract

The invention discloses a preparation method of a stasis-phlegm type ovarian cyst animal model. The preparation method comprises the following steps of (1) applying an estradiol benzoate solution to a rat in estrus with the application dose of 1.5ml / 100g once every 5 days for 4 times continuously, wherein the concentration of the estradiol benzoate solution is 3.75 mg / ml; (2) performing intragastric administration with 1ml / 100g high-fat milk once every day for 20 times continuously from the day when the estradiol benzoate solution is applied for the first time, wherein each 100ml of high-fat milk contains the following raw materials by weight: 20g of cholesterol, 3g of sodium cholate, 35g of animal fat, 20ml of propylene glycol, 10ml of tween-80 and the balance being water. The preparation method has the advantage that the stasis-phlegm type ovarian cyst animal model is successfully induced in a manner of combination modeling of subcutaneous injection of estradiol and intragastric administration of high-fat milk; the preparation method has excellent application prospect.

Description

technical field [0001] The invention relates to a method for preparing an animal model of ovarian cyst with mixed phlegm and blood stasis. Background technique [0002] Ovarian cysts (ovarian cysts, OC) refer to the formation of lumps in or on the surface of the ovary. The substance in the lump is usually liquid, sometimes it may be solid, or a mixture of liquid and solid. Ovarian cyst is the most common gynecological disease, which can occur in any female age, especially in women of childbearing age. There are many aspects to the pathogenesis of ovarian cysts. The main reason is that hypothalamic-pituitary-ovarian dysfunction leads to pathological gonadotropin secretion, which affects the production, development, and release of follicles, and eventually forms ovarian cysts. Ovarian cysts belong to the category of "accumulation", "sickness" and "intestinal pain" in traditional Chinese medicine. Its formation is mostly caused by the invasion of six evils, damage of seven emo...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K35/12A61K49/00A61K31/565A61K31/575A61K35/35
CPCA01K67/027A01K2207/20A01K2227/105A01K2267/035A61K35/12A61K49/0008
Inventor 张仲林凌保东钟玲臧志和袁明勇辛志伟彭延娟颜晓燕盛艳梅
Owner CHENGDU MEDICAL COLLEGE
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