Method for quickly determining residual hydrogen peroxide

A technology for rapid determination of hydrogen peroxide, which is applied in the direction of material analysis by observing the influence of chemical indicators, and analysis by making materials undergo chemical reactions, which can solve the problems of peroxidase variability and inactivation, etc. Achieve the effects of good stability, pollution prevention and fast response time

Active Publication Date: 2013-12-11
GUANGDONG HUANKAI MICROBIAL SCI & TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

In this method, peroxidase is highly denatur

Method used

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  • Method for quickly determining residual hydrogen peroxide

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Experimental program
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Effect test

Embodiment 1

[0038] Prepare with pure water containing 0.05g / L horseradish peroxidase, 2.0g / L TMB hydrochloride, 0.037mol / L citric acid, 0.126mol / L disodium hydrogen phosphate, 10 g / L trehalose, 5 g A mixed chromogenic solution of / L glycine, 3g / LBSA, 10g / LPEG4000. Soak the chromatographic filter paper completely in the above mixture for about 10 minutes, take out the filter paper, and dry it in vacuum at a temperature lower than 30°C. Cut the filter paper into small pieces of 5×6 mm and glue them on a strip of 5×80 mm One end of the bottom plate is made into test paper 1# for the determination of hydrogen peroxide residue.

Embodiment 2

[0040] Prepare with pure water containing 0.01g / L horseradish peroxidase, 1.0g / L TMB sulfate, 0.028mol / L citric acid, 0.11mol / L disodium hydrogen phosphate, 10g / L trehalose, 5g / L essence Amino acid, 2g / LBSA, 1g / LHSA, 10g / LPVP K30 mixed chromogenic solution. Soak the chromatographic filter paper completely in the above mixture for 5 minutes, take out the filter paper, and dry it under vacuum at a temperature lower than 30°C. Cut the filter paper into small pieces of 5×5 mm and glue them on a One end of the bottom plate is made into test paper 2# for the determination of hydrogen peroxide residue.

Embodiment 3

[0042] Prepare with pure water containing 0.025g / L horseradish peroxidase, 1.5g / L TMB propanesulfonate, 0.044mol / L citric acid, 0.14mol / L disodium hydrogen phosphate, 2g / L sucrose, 2g / L Mixed chromogenic solution of trehalose, 2g / L glycine, 1g / LHSA, 4g / LPEG6000. Completely immerse the qualitative in the above mixture for about 20 minutes, take out the filter paper, and dry it in vacuum at a temperature lower than 30°C. Cut the filter paper into small pieces of 5×5 mm, and glue them on a 5×50mm strip of plastic One end of the hard board is made into test paper 3# for the determination of hydrogen peroxide residue.

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Abstract

The invention discloses a method for quickly determining residual hydrogen peroxide. According to the method, residual hydrogen peroxide in a to-be-determined sample is quickly determined by detection paper; the method capable of quickly detecting the content of residual peracetic acid in the sample on site is good in stability, easy and simple to operate, economical, practical, short in response time and high in sensitivity; and the lowest detected content of hydrogen peroxide is 0.5mg/L. In addition, the method can be widely applied to the industries of food, daily chemical and medical treatment, and can quickly and effectively monitor the content of residual hydrogen peroxide in various disinfected or bleached samples.

Description

technical field [0001] The invention relates to a rapid determination method for residual hydrogen peroxide. Background technique [0002] Hydrogen peroxide is a strong oxidizing agent, which has the functions of disinfection, sterilization and bleaching, and is widely used in industrial and medical fields. In the food industry, hydrogen peroxide is mainly used for the disinfection of flexible packaging paper, disinfectant in canning factories, sterilization of milk and milk products, etc. Excessive residual concentration of hydrogen peroxide in food has the risk of carcinogenesis to humans, and may also accelerate human aging. Therefore, the "Hygienic Standards for the Use of Food Additives" GB2760-2002 stipulates that hydrogen peroxide can only be used as a food additive in fresh milk and bagged dried tofu, and at the same time it is stipulated that there should be no residue in bagged dried tofu. Hydrogen peroxide must be strictly approved by the Ministry of Health. Ho...

Claims

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Application Information

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IPC IPC(8): G01N21/78
Inventor 阙绍辉秦惠邓金花黄报亮
Owner GUANGDONG HUANKAI MICROBIAL SCI & TECH
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