Method for reducing tissue culture production cost of hosta plantagineu

A technology for flower and leaf hosta and production cost, which is applied in the field of reducing the cost of tissue culture production of flower and leaf hosta, can solve the problems of few introduction of flower and leaf hosta, limited application speed and high production cost, and achieves improvement of transplanting survival rate and reduction of production. Manage cost and save watering effect

Active Publication Date: 2013-12-18
HEBEI ACAD OF FORESTRY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Hosta mosaic is affected by factors such as the small number of introductions and the slow speed of ramets, which limits its application speed
Tissue culture is an effective way to maintain the excellent traits of varieties and to quickly scale up production, but the production cost is relatively high

Method used

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  • Method for reducing tissue culture production cost of hosta plantagineu
  • Method for reducing tissue culture production cost of hosta plantagineu
  • Method for reducing tissue culture production cost of hosta plantagineu

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) In spring, take the young shoots of Hosta japonica, remove part of the leaves and roots, rinse them with tap water, put them on the workbench, soak them in 75% alcohol for 30 seconds, and use 0.1% HgCl 2 Soak for 10min, rinse with sterile water 4~5 times, cut the buds into about 1cm and inoculate them in bud induction medium 1 / 3MS+6-BA 3mg.L -1 +IBA 0.1 mg.L -1 + white sugar 30 g.L -1 + agar 4 g.L -1 above, pH5.8~6.0, culture temperature 23~25℃, light time 12~14h·d -1 , light intensity 1000~2000Lux, cultivated for 30 days to obtain tissue blocks with adventitious buds.

[0020] (2) Set the size to 1cm 3 Left and right tissue pieces with adventitious buds were inoculated in induction differentiation medium MS+6-BA 2.0mg.L -1 +IBA 0.1mg.L -1 + white sugar 30 g.L -1 + agar 4 g.L -1 Above, pH5.8~6.0, the culture conditions are the same as (1). Each test group was inoculated with 5 buds, and a total of 10 test groups were established. After 25 days, the newly ad...

Embodiment 2

[0034] The bud induction medium used in this example is 1 / 3MS+6-BA4mg.L -1 +IBA 0.3 mg.L -1 + white sugar 40 g.L -1 + agar 5 g.L -1 , the differentiation and proliferation medium is MS+6-BA 3.0mg.L -1 +IBA 0.3 mg.L -1 + white sugar 45 g.L -1 + agar 5 g.L -1 , pH5.8~6.0; the rooting medium used is MS or 1 / 2MS+IBA0~0.5 mg.L -1 + white sugar 40 g.L -1 + agar 4 g.L -1 , pH 5.8 ~ 6.0, harden seedlings in the seedling hardening matrix for 30d, other conditions are the same as in Example 1, the average value-added coefficient is 4.10 in the bud differentiation step, the average rooting rate is 96.7% in the rooting step, and the average rooting bar number is 7.58 , the cost of each stage is equivalent to that of Example 1.

Embodiment 3

[0036] The bud induction medium used in this example is 1 / 3MS+6-BA5mg.L -1 +IBA 0.5 mg.L -1 + white sugar 45 g.L -1 + agar 6 g.L -1 , the differentiation and proliferation medium is MS+6-BA 4.0mg.L -1 +IBA 0.5 mg.L -1 + white sugar 45 g.L -1 + agar 5 g.L -1 , pH5.8~6.0; The rooting medium used is MS, harden seedlings in the seedling hardening matrix for 40d, other conditions are the same as embodiment 1, the average value-added coefficient is 4.08 in its bunchy bud differentiation step, and the average rooting rate is 94.8% in the rooting step , the average rooting bar number is 7.50, and the cost of each stage is suitable with embodiment 1.

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Abstract

The invention discloses a method for reducing tissue culture production cost of hosta plantagineu. The method comprises the steps: obtaining a tissue block with an adventitious bud, differentiating and multiplying, rooting, transplanting, acclimatizing, planting in a large land, and the like. According to the method, through screening a proper culture medium and selecting a proper culturing and transplanting method, the culture efficiency of the hosta plantagineu is improved, and the production cost is lowered.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for reducing the production cost of hosta mosaic tissue culture. technical background [0002] Mosaic hosta ( Hosta sieboldiana ) is a perennial herbaceous flower of Liliaceae, which is a new variety of hosta introduced from abroad in recent years. Hosta mosaic grows vigorously, is cold-resistant and shade-loving, avoids direct sunlight, and grows well under trees or on the north side of buildings. Mosaic hosta not only has the excellent characteristics of hosta, but also has bright leaves, clear color stripes on the double-colored leaves, and obvious leaf veins. It is a very good potted and greening variety. [0003] Hosta mosaic is affected by factors such as the small number of introductions and the slow speed of ramets, which limits its application speed. Tissue culture is an effective way to maintain the excellent traits of varieties and produce ra...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
Inventor 赵玉芬储博彦尹新彦李金霞刘满光
Owner HEBEI ACAD OF FORESTRY SCI
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