Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for reducing tissue culture production cost of hosta plantagineu

A technology of hosta mosaic and production cost, which is applied in the field of reducing the production cost of hosta mosaic tissue culture, can solve the problems of small number of introduced hosta mosaic, limited application speed, and high production cost, so as to improve the survival rate of transplanting and reduce the production cost. Cost management and water saving effect

Active Publication Date: 2015-07-01
HEBEI ACAD OF FORESTRY SCI
View PDF3 Cites 0 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] Hosta mosaic is affected by factors such as the small number of introductions and the slow speed of ramets, which limits its application speed
Tissue culture is an effective way to maintain the excellent traits of varieties and to quickly scale up production, but the production cost is relatively high

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for reducing tissue culture production cost of hosta plantagineu
  • Method for reducing tissue culture production cost of hosta plantagineu
  • Method for reducing tissue culture production cost of hosta plantagineu

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] (1) In spring, take the young shoots of Hosta japonica, remove part of the leaves and roots, rinse them with tap water, put them on the workbench, soak them in 75% alcohol for 30 seconds, and use 0.1% HgCl 2 Soak for 10min, rinse with sterile water 4~5 times, cut the buds into about 1cm and inoculate them in bud induction medium 1 / 3MS+6-BA 3mg.L -1 +IBA 0.1 mg.L -1 + white sugar 30 g.L -1 + agar 4 g.L -1 above, pH5.8~6.0, culture temperature 23~25℃, light time 12~14h·d -1 , light intensity 1000~2000Lux, cultivated for 30 days to obtain tissue blocks with adventitious buds.

[0020] (2) Set the size to 1cm 3 Left and right tissue pieces with adventitious buds were inoculated in induction differentiation medium MS+6-BA 2.0mg.L -1 +IBA 0.1mg.L -1 + white sugar 30 g.L -1 + agar 4 g.L -1 Above, pH5.8~6.0, the culture conditions are the same as (1). Each test group was inoculated with 5 buds, and a total of 10 test groups were established. After 25 days, the newly ad...

Embodiment 2

[0034] The bud induction medium used in this example is 1 / 3MS+6-BA4mg.L -1 +IBA 0.3 mg.L -1 + white sugar 40 g.L -1 + agar 5 g.L -1 , the differentiation and proliferation medium is MS+6-BA 3.0mg.L -1 +IBA 0.3 mg.L -1 + white sugar 45 g.L -1 + agar 5 g.L -1 , pH5.8~6.0; the rooting medium used is MS or 1 / 2MS+IBA0~0.5 mg.L -1 + white sugar 40 g.L -1 + agar 4 g.L -1 , pH 5.8 ~ 6.0, harden seedlings in the seedling hardening matrix for 30d, other conditions are the same as in Example 1, the average value-added coefficient is 4.10 in the bud differentiation step, the average rooting rate is 96.7% in the rooting step, and the average rooting bar number is 7.58 , the cost of each stage is equivalent to that of Example 1.

Embodiment 3

[0036] The bud induction medium used in this example is 1 / 3MS+6-BA5mg.L -1 +IBA 0.5 mg.L -1 + white sugar 45 g.L -1 + agar 6 g.L -1 , the differentiation and proliferation medium is MS+6-BA 4.0mg.L -1 +IBA 0.5 mg.L -1 + white sugar 45 g.L -1 + agar 5 g.L -1 , pH5.8~6.0; The rooting medium used is MS, harden seedlings in the seedling hardening matrix for 40d, other conditions are the same as embodiment 1, the average value-added coefficient is 4.08 in its bunchy bud differentiation step, and the average rooting rate is 94.8% in the rooting step , the average rooting bar number is 7.50, and the cost of each stage is suitable with embodiment 1.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention discloses a method for reducing the production cost of hosta mosaic tissue culture, which includes the steps of obtaining tissue blocks with adventitious buds, differentiation and proliferation, rooting, transplanting and domestication, and field planting. The suitable cultivation and transplanting method improves the cultivation efficiency of the hosta mosaic and reduces the production cost.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture, in particular to a method for reducing the production cost of hosta mosaic tissue culture. technical background [0002] Mosaic hosta ( Hosta sieboldiana ) is a perennial herbaceous flower of Liliaceae, which is a new variety of hosta introduced from abroad in recent years. Hosta mosaic grows vigorously, is cold-resistant and shade-loving, avoids direct sunlight, and grows well under trees or on the north side of buildings. Mosaic hosta not only has the excellent characteristics of hosta, but also has bright leaves, clear color stripes on the double-colored leaves, and obvious leaf veins. It is a very good potted and greening variety. [0003] Hosta mosaic is affected by factors such as the small number of introductions and the slow speed of ramets, which limits its application speed. Tissue culture is an effective way to maintain the excellent traits of varieties and produce rapi...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Patents(China)
IPC IPC(8): A01H4/00
Inventor 赵玉芬储博彦尹新彦李金霞刘满光
Owner HEBEI ACAD OF FORESTRY SCI
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com