Synthesis method of peroxide mimic enzyme nano-catalysis particles

A technology of peroxide and nano-catalysis, applied in the direction of metal/metal oxide/metal hydroxide catalyst, chemical instrument and method, physical/chemical process catalyst, etc., can solve the problem that the function of optical signal converter cannot be reflected, static electricity Repulsion instability and other problems, to achieve a significant surface plasmon resonance absorption peak and low price, enhance the effect of optical signal conversion

Inactive Publication Date: 2013-12-18
SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] The peroxide mimetic enzymes based on gold nanoparticles are mainly nanoparticles modified by positive charges, such as nanoparticles modified by mercaptoamines. The function of the converter does not reflect the

Method used

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  • Synthesis method of peroxide mimic enzyme nano-catalysis particles
  • Synthesis method of peroxide mimic enzyme nano-catalysis particles
  • Synthesis method of peroxide mimic enzyme nano-catalysis particles

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Experimental program
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Embodiment 1

[0029] All the experimental containers involved in this experiment were washed sequentially with tap water, reverse osmosis water, soaked in MilliQ water overnight, washed with aqua regia, soaked in MilliQ water, washed, and dried in an oven. In a three-neck round bottom flask with a stirrer and a reflux tube, add 500 mM HAuCl with a concentration of 0.8 mM prepared with MilliQ water 4 solution with a concentration of 0.2mMNa 2 PdCl 4 Solution, the middle port of the three-neck round bottom flask is connected to the stirrer, one oblique port is connected to the reflux pipe, and the other oblique port is plugged with a ground glass stopper. Continue to heat the solution in the three-necked round-bottom flask, and at the same time turn on the agitator to stir the solution. After the solution boils, when the reflux flow in the reflux tube tends to be stable, pour 50 mL of the previously prepared sodium citrate solution with a concentration of 38.8 mM into the reaction The final...

Embodiment 2

[0032] All the experimental containers involved in this experiment were washed sequentially with tap water, reverse osmosis water, soaked in MilliQ water overnight, washed with aqua regia, soaked in MilliQ water, washed, and dried in an oven. Add 500 mL of 0.6 mM HAuCl prepared with MilliQ water to a three-necked round bottom flask with a stirrer and a reflux tube 4 solution and 0.4mMPd(NO 3 ) 2Solution, the middle port of the three-neck round bottom flask is connected to the stirrer, one oblique port is connected to the reflux pipe, and the other oblique port is plugged with a ground glass stopper. Continue to heat the solution in the three-neck round bottom flask, and at the same time turn on the stirrer to stir the solution. After the solution boils, when the reflux flow in the reflux tube tends to be stable, pour 50mL of sodium citrate solution with a concentration of 38.8mL prepared in advance into the reaction The final concentration of sodium citrate in the container ...

Embodiment 3

[0035] All the experimental containers involved in this experiment were washed sequentially with tap water, reverse osmosis water, soaked in MilliQ water overnight, washed with aqua regia, soaked in MilliQ water, washed, and dried in an oven. Add 500 mL of 0.4 mM HAuCl prepared with MilliQ water to a three-necked round bottom flask with a stirrer and a reflux tube 4 solution and PdSO at a concentration of 0.6mM 4 Solution, the middle port of the three-neck round bottom flask is connected to the stirrer, one oblique port is connected to the reflux pipe, and the other oblique port is plugged with a ground glass stopper. Continue to heat the solution in the three-necked round-bottom flask, and at the same time turn on the agitator to stir the solution. After the solution boils, when the reflux flow in the reflux tube tends to be stable, pour 50 mL of the previously prepared sodium citrate solution with a concentration of 38.8 mM into the reaction The final concentration of sodiu...

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Abstract

The invention discloses a synthesis method of peroxide mimic enzyme nano-catalysis particles. The synthesis method comprises the following steps: performing a synthesis reaction on a solution containing a gold source and a palladium source in the presence of a stabilizing agent and a reducing agent to obtain nano gold-palladium alloy particles serving as the peroxide mimic enzyme nano-catalysis particles, wherein the molar ratio of the gold source to the palladium source is (1:5)-(5:1); the molar ratio of the reducing agent to the gold source and palladium source together is (5:1)-(1:20); the stabilizing agent is citrate; the reducing agent is citrate or NaBH4. The synthesis method disclosed by the invention is used for synthesizing stable nano gold-palladium alloy particles with surface plasmon resonance effect and peroxide mimic enzyme activity. The nano gold-palladium alloy particles have stable properties and obvious and stable surface plasmon resonance peak; the optimal pH for the enzyme activity is 3, and the requirement for ion strength in the reaction is not high; the synthesis method can be applied to the peroxide catalysis in a protein denaturation environment and has excellent application potential in the fields of analysis and molecular computation.

Description

technical field [0001] The invention relates to a method for synthesizing peroxide-mimicking enzyme nano-catalyst particles, which belongs to the method for synthesizing new nano-materials. Background technique [0002] Mimetic enzymes are a class of artificially synthesized catalysts that have no protein structure but have similar catalytic activity to natural enzymes. In the study of simulated enzymes, peroxide simulated enzymes have been developed in depth because they can be widely used in the field of analysis and detection (enzyme-catalyzed color reactions, chemiluminescent reactions, electrochemical signal conversion, etc.). For a long time, the simulated enzyme catalytic activity of nanoparticles has not been discovered. Until recent years, materials such as nano-ferric oxide, rare earth nano-ions, nano-cobalt tetraoxide, multi-walled carbon nanotubes, and punctual modified gold nanoparticles have all discovered peroxides. The simulated enzyme activity greatly expan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): B01J23/52
Inventor 梁晓声熊海荣王海英郭小华汪文俊
Owner SOUTH CENTRAL UNIVERSITY FOR NATIONALITIES
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