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Test strip for rapidly detecting trace sulfachlorpyridazine and preparation method thereof

A technology of sulfachloropyridazine and test strips, applied in the field of immunochemical detection of veterinary drug residues

Inactive Publication Date: 2015-07-01
HENAN BAIAO BIOLOGICAL ENG CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These methods have high sensitivity and strong specificity for the detection of sulfachloropyridazine, but the samples need to undergo a series of complicated treatment and purification, which is cumbersome and time-consuming. It takes at least 2 days from sample pretreatment to test results; at the same time, this detection method requires large-scale specialized Instruments and equipment and professional technicians operate, unable to conduct on-site testing, poor timeliness, and difficult to promote
The immunological detection method has semi-quantitative and certain quantitative capabilities, and can provide preliminary information on the analyte. This method has high sensitivity and relatively simple analysis process. It has unique advantages in the screening of sulfachloropyridazine and needs to be developed first. detection technology, but the immunological detection method of sulfachloropyridazine urgently needs to be studied and solved

Method used

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  • Test strip for rapidly detecting trace sulfachlorpyridazine and preparation method thereof
  • Test strip for rapidly detecting trace sulfachlorpyridazine and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Embodiment one: the test strip of rapid detection trace sulfachloropyridazine, see figure 1 , figure 2 . In the figure, the support layer 1 is made of plastic thin strips, the adsorption fiber layer 2 is made of glass fiber cotton, the gold-labeled antibody fiber layer 3 is adsorbed with anti-sulfachloropyridazine monoclonal antibody, and the cellulose membrane layer 4 is made of nitrocellulose The plain film, the water-absorbing material layer 5 is made of water-absorbing filter paper, and the layers numbered 2, 3, 4, and 5 are pasted and fixed on the support layer 1 in sequence from right to left, and the fibers at the intersections cross and penetrate each other. A detection blot 6 and a control blot 7 are arranged on the cellulose membrane layer 4, the detection blot is printed with bovine serum albumin (BSA) solution coupled with sulfachloropyridazine, and the control blot is printed with goat anti-antibody solution, Two blots aligned in parallel to form blotte...

Embodiment 2

[0054] Embodiment 2: The structure of the test strip is basically the same as that of Embodiment 1, the difference is that the polyclonal antibody against sulfachloropyridazine is adsorbed on the gold-labeled antibody fiber layer, the adsorption fiber layer is made of nylon membrane, and the cellulose membrane The layer is made of pure cellulose film, the test blot and the control blot are both "ten", and the protective film at the handle end covering the water-absorbing material layer is blue.

[0055]For the detection of milk samples, the milk samples can be directly diluted with physiological saline to make a 1:2~5 suspension of the sample to be tested;

[0056] Result judgment: (a) Positive If there is a brown-red blot on the cellulose membrane " ten ", indicating that the test result is positive, indicating that sulfachloropyridazine is contained in the sample to be tested; (b) negative if there are two brown-red blots on the cellulose membrane" ten ", expression test ...

Embodiment 3

[0057] Embodiment three: the sulfachloropyridazine test paper strip is basically the same as embodiment one, the difference is:

[0058] The absorbent fiber layer is made of PVDF membrane, the carrier protein solution coupled with sulfachloropyridazine is chicken ovalbumin (OVA), and the control blot is printed on the cellulose membrane with rabbit anti-mouse IgG antibody solution, and the cellulose membrane The layer is made of carboxylated cellulose film, the protective film on the handle end covering the water-absorbing material layer is green, and both the detection blot and the control blot are "┬".

[0059] Detection of blood samples: Extract serum and dilute it with normal saline to make a 1:2-10 sample to be tested.

[0060] Result judgment: (a) positive if there is a brown-red blot "┬" on the cellulose membrane, it means that the test result is positive, indicating that sulfachloropyridazine is contained in the sample to be tested; (b) negative if there is a blot on t...

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Abstract

The present invention involves a test note and its preparation method for fast detection of sulfa chloride. The bottom layer is the support layer, the middle layer is the adsorption layer, and the protective layer is fixed on the adsorption layer.The golden standard antibody fiber layer, cellulose membrane layer and water absorption material layer. On the fibrin membrane layer, there is a detection mark printed on the protein solution printed by the protein solution of the protein solution of sulfa.Anti -rabbit IgG antibody solution printed in the control seal, the golden standard antibody is a sulfonamine antibody marked by colloidal gold.The test strips of the present invention are strong, high, simple, intuitive, accurate, wide, wide, low in cost, simple operation, can meet the needs of personnel at different levels, have broad market prospects, and are easy to promote and applied.

Description

technical field [0001] The invention belongs to the field of immunochemical detection of veterinary drug residues, in particular to an immunocolloidal gold test strip for rapidly detecting trace amounts of sulfachloropyridazine and a preparation method thereof. technical background [0002] Sulfachloropyridazine (sulfachloropyridazine, SPDZ) molecular formula C 10 h 9 ClN 4 o 2 S, with a molecular weight of 284.72, is a kind of synthetic antibacterial drug that inhibits nucleic acid synthesis. It belongs to a broad-spectrum antibacterial drug. It has the characteristics of low toxicity and easy absorption after oral administration. Antibacterial effect. Due to the low price and effective antibacterial effect of sulfa drugs, they are widely used in animal husbandry as preventive and therapeutic drugs. However, the unreasonable use or even abuse of sulfachloropyridazine will cause the emergence of drug-resistant strains on the one hand, leading to the pollution of animal ...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N33/577G01N33/531
Inventor 张改平赵东王栋职爱民王方雨胡骁飞
Owner HENAN BAIAO BIOLOGICAL ENG CO LTD
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