Application of 4', 5, 7-genistein in preparing rotavirus resisting medicine

A technology of trihydroxyisoflavone and rotavirus, which is applied in the field of compounds, can solve the problems such as genistein, which has not been seen yet, and achieve the effect of meeting the needs of clinical treatment, solving the poor effect and ensuring the health of the body

Inactive Publication Date: 2013-12-25
ARMY MEDICAL UNIV
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Problems solved by technology

However, so far, there have been no relevant reports that Genistei

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  • Application of 4', 5, 7-genistein in preparing rotavirus resisting medicine
  • Application of 4', 5, 7-genistein in preparing rotavirus resisting medicine
  • Application of 4', 5, 7-genistein in preparing rotavirus resisting medicine

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Embodiment Construction

[0019] Hereinafter, preferred embodiments of the present invention will be described in detail with reference to the accompanying drawings. Experimental methods not indicating specific conditions in the preferred embodiments are generally in accordance with conventional conditions, such as described in the Molecular Cloning Experiment Guide (Third Edition, J. Sambrook et al., translated by Huang Peitang, Science Press, 2002) conditions, or as recommended by the manufacturer.

[0020] 1. Quantitative proteomics method to analyze the protein change spectrum of host cells infected by rotavirus

[0021] A quantitative proteomics method-cell culture stable isotope amino acid labeling (SILAC) was used to analyze the changes of Caco-2 protein expression profile in intestinal epithelial cells after RV infection. First prepare "light" DMEM medium, "medium" DMEM medium and "heavy" DMEM medium, wherein "light" DMEM medium is DMEM medium in which lysine and arginine are replaced by L-Lys...

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Abstract

The invention discloses application of 4', 5, 7-genistein in preparing rotavirus resisting medicine. The genistein can restrain damage of tyrosine protein kinase to cell junction by restraining activation, caused by rotavirus infection, of tyrosine protein kinase of intestinal epithelial cells, so that the intestinal epithelial cells are prevented from being damaged by rotavirus, the completeness of intestinal mucosa is protected, the symptom of rotavirus diarrhea is obviously reduced, and the lethality is reduced. Thus, when the genistein is used as a main active ingredient, high-efficient and low-toxicity specificity rotavirus diarrhea resisting medicine can be researched and developed hopefully.

Description

technical field [0001] The invention belongs to the new application of compounds, in particular to the application of 4',5,7-trihydroxy isoflavone (Genistein) in the preparation of anti-rotavirus drugs. Background technique [0002] Rotavirus (Rotavirus, RV) belongs to the family Reoviridae and is a non-enveloped double-stranded RNA (dsRNA) virus. Each of the 11 dsRNAs wraps 1 molecule of RNA-dependent RNA polymerase (VP1) and 1 molecule of guanylic acid and Methyltransferase (VP3) is located in the core of virus particles, encoding 12 proteins, including 6 structural proteins (VP1-4, 6-7) and 6 non-structural proteins (NSP1-6). VP2 constitutes the inner capsid of rotavirus to enclose the viral genome; the middle capsid composed of VP6 wraps around VP2 to form a rotavirus double-layer capsid particle (DLP); the outer capsid composed of VP4 and VP7 wraps around DLP to form Intact rotavirus triple-layered capsid particle (TLP). The rotavirus enters the cell through the cell ...

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Application Information

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IPC IPC(8): A61K31/352A61P31/14A61P1/12
CPCA61K31/353A61P1/12A61P31/14
Inventor 何海洋李晋涛吴玉章
Owner ARMY MEDICAL UNIV
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