Preparation method of silk fibroin/metal nanoparticle composite system

A technology of metal nanoparticles and silk fibroin, which is applied in the field of preparation of the composite system of silk fibroin and inorganic metal nanoparticles, to achieve the effect of mature extraction technology, wide application range and good stability

Inactive Publication Date: 2013-12-25
TAIYUAN UNIV OF TECH
View PDF10 Cites 38 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Combining the respective advantages of silk fibroin and metal nanoparticles, a silk fibroin/metal nanoparticle composite system with g

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Preparation method of silk fibroin/metal nanoparticle composite system
  • Preparation method of silk fibroin/metal nanoparticle composite system
  • Preparation method of silk fibroin/metal nanoparticle composite system

Examples

Experimental program
Comparison scheme
Effect test

Example Embodiment

[0023] Embodiment 1:

[0024] (1) Preparation of silk fibroin aqueous solution:

[0025] 20 g of pupated and peeled silkworm cocoons were placed in 2 L of 0.5wt% Na 2 CO 3 In aqueous solution, boiled for 0.5 hours to remove surface sericin, washed three times with deionized water, and dried to obtain degummed silk fibroin fibers. Calcium chloride-ethanol-water (molar ratio 1: 2: 8) ternary system was used to dissolve degummed silk fibroin fibers. The time was 3 h, and the silk fibroin solution was obtained. The solution was vacuum filtered to remove impurities, and then put into a dialysis bag with a molecular weight cut-off of 8000-14000 for continuous dialysis for three days to remove salt ions to obtain a pure silk fibroin aqueous solution. Adjust the concentration of silk fibroin solution to 0.01 wt%.

[0026] (2) Preparation of metal nanoparticles:

[0027] In a 250 mL single-neck flask, add 100 mL of 1 mM HAuCl 4 The solution was heated and boiled, and 9.34 mL ...

Example Embodiment

[0040] Embodiment 2:

[0041] (1) Preparation of silk fibroin aqueous solution:

[0042] Put 20 g of raw silk into 1 L of 1 wt% Na 2 CO 3 In aqueous solution, boiled for 1 hour to remove surface sericin, washed three times with deionized water, and dried to obtain degummed silk fibroin fibers. The degummed silk fibroin was dissolved in a 9 M LiBr aqueous solution. The weight-to-volume ratio of silk fibroin to LiBr aqueous solution was 10:1, the dissolution temperature was 40 °C, and the dissolution time was 2 h to obtain a silk fibroin solution. The silk fibroin solution was vacuum filtered to remove impurities, and then put into a dialysis bag with a molecular weight cut-off of 8000-14000 for continuous dialysis for three days to remove salt ions to obtain a pure silk fibroin aqueous solution. Adjust the silk fibroin solution concentration to 0.05 wt%.

[0043] (2) Prepare an aqueous solution of metal nanoparticles:

[0044] In a 250 mL single-neck flask, add 100...

Example Embodiment

[0054] Embodiment 3:

[0055] (1) Preparation of silk fibroin aqueous solution:

[0056] 50 g of pupa-removed and thinned silkworm cocoons were placed in 3 L of 0.5wt% Na 2 CO 3 In aqueous solution, boiled for 2 hours to remove surface sericin, washed three times with deionized water, and dried to obtain degummed silk fibroin fibers. The degummed silk fibroin fibers were dissolved in a ternary system of calcium chloride-ethanol-water (molar ratio 1: 2: 8). The dissolution time was 1 h to obtain a silk fibroin solution. The solution was vacuum filtered to remove impurities, and then put into a dialysis bag with a molecular weight cut-off of 8000-14000 for continuous dialysis for three days to remove salt ions to obtain a pure silk fibroin aqueous solution.

[0057] Adjust the silk fibroin solution concentration to 0.15 wt%.

[0058] (2) Prepare an aqueous solution of metal nanoparticles:

[0059] In a 250 mL single-neck flask, add 100 mL of 1 mM H 2 PtCl 6 The solution...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

PropertyMeasurementUnit
The average particle sizeaaaaaaaaaa
The average particle sizeaaaaaaaaaa
The average particle sizeaaaaaaaaaa
Login to view more

Abstract

The invention provides a preparation method of a silk fibroin/metal nanoparticle composite system, belonging to the technical field of nanocomposite materials and preparation methods thereof. The preparation method comprises the steps of fully mixing metal nanoparticles with silk fibroin in a weight ratio of (1:9)-(9:1) so that silk fibroin is non-covalently adsorbed on the surfaces of the metal nanoparticles, thus obtaining the silk fibroin/metal nanoparticle composite system. The metal nanoparticles in the composite system prepared by the preparation method can not aggregate under the conditions that the pH value is 2-11 and the salinity is 0-1000mM, thus improving the stability and biocompatibility of the metal nanoparticles in the physiological environment. The silk fibroin/metal nanoparticle composite system provided by the invention is simple to prepare, is low in cost, has good stability and biocompatibility and has wide application prospects in the biomedical fields of drug sustained release, gene transmission, tissue engineering and the like.

Description

technical field [0001] The invention belongs to the technical field of preparation of nanocomposite materials, and in particular relates to a preparation method of a composite system of silk fibroin and inorganic metal nanoparticles. Background technique [0002] Due to a series of excellent properties, such as magnetic properties, electrical properties, and optical properties, metal nanoparticles have been widely used as nanomaterials in the fields of biology, chemistry, and medicine. In the field of biomedicine, including sustained drug release, gene delivery, biomolecular diagnosis and detection, and biosensors, most of the nanoparticles used in the field require the ability to operate in physiological environments such as a wide pH range and high salt concentration. Stable dispersion in the environment, in addition, biocompatibility is also a prerequisite for the application of nanoparticles. However, metal nanoparticles directly synthesized in aqueous solution usually ...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
IPC IPC(8): C08L89/00C08K3/08C08K9/10C08K3/22C08J3/05C08J3/11
Inventor 贾兰朱晶心卫爱丽郭丽
Owner TAIYUAN UNIV OF TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products