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Preparation method of silk fibroin/metal nanoparticle composite system

A technology of metal nanoparticles and silk fibroin, which is applied in the field of preparation of the composite system of silk fibroin and inorganic metal nanoparticles, to achieve the effect of mature extraction technology, wide application range and good stability

Inactive Publication Date: 2013-12-25
TAIYUAN UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Combining the respective advantages of silk fibroin and metal nanoparticles, a silk fibroin / metal nanoparticle composite system with good biocompatibility and physiological environment stability can be prepared in a simple and easy way, which has not been reported yet.

Method used

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  • Preparation method of silk fibroin/metal nanoparticle composite system
  • Preparation method of silk fibroin/metal nanoparticle composite system
  • Preparation method of silk fibroin/metal nanoparticle composite system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach 1

[0024] (1) Preparation of silk fibroin aqueous solution:

[0025] Put 20 g of silkworm cocoons that had been de-pupaed and stripped into thin layers into 2 L of 0.5 wt% Na 2 CO 3 In an aqueous solution, boil for 0.5 hours to remove surface sericin, wash with deionized water three times, and dry to obtain degummed silk fibers. Use calcium chloride-ethanol-water (molar ratio 1: 2: 8) ternary system to dissolve the degummed silk fiber, the weight-to-volume ratio of the degummed silk fiber to the ternary solvent system is 10: 1, and the dissolution temperature is 60°C. The time is 3 h, and the silk fiber solution is obtained. The solution is vacuum filtered to remove impurities, and then put into a dialysis bag with a molecular weight cut-off of 8000-14000 for continuous dialysis for three days to remove salt ions to obtain a pure silk fibroin aqueous solution. Adjust the concentration of silk fibroin solution to 0.01wt%.

[0026] (2) Preparation of metal nanoparticles:

...

Embodiment approach 2

[0041] (1) Preparation of silk fibroin aqueous solution:

[0042] Put 20 g of raw silk into 1 L of 1 wt% Na 2 CO 3 in aqueous solution, boiled for 1 hour to remove surface sericin, washed three times with deionized water, and dried to obtain degummed silk fibroin fibers. The degummed silk fibers were dissolved in 9 M LiBr aqueous solution, the weight-to-volume ratio of silk fibers to LiBr aqueous solution was 10:1, the dissolution temperature was 40°C, and the dissolution time was 2 hours to obtain a silk fiber solution. Vacuum filter the silk fiber solution to remove impurities, and then put it into a dialysis bag with a molecular weight cut-off of 8000-14000 for continuous dialysis for three days to remove salt ions to obtain a pure silk fibroin aqueous solution. Adjust the concentration of silk fibroin solution to 0.05 wt%.

[0043] (2) Preparation of aqueous solution of metal nanoparticles:

[0044] In a 250 mL one-necked flask, add 100 mL of water, add 0.05 mmol Ag...

Embodiment approach 3

[0055] (1) Preparation of silk fibroin aqueous solution:

[0056] 50 g of silkworm cocoons that had been removed from the pupae and stripped into thin layers were placed in 3 L of 0.5 wt% Na 2 CO 3 In aqueous solution, boiled for 2 hours to remove surface sericin, washed three times with deionized water, and dried to obtain degummed silk fibers. Dissolve the degummed silk fibroin fibers in a ternary system of calcium chloride-ethanol-water (molar ratio 1: 2: 8), the weight-to-volume ratio of the silk fibers to the ternary solvent system is 10: 1, and the dissolution temperature is 80°C. The dissolution time was 1 h, and the silk fiber solution was obtained. The solution is vacuum filtered to remove impurities, and then put into a dialysis bag with a molecular weight cut-off of 8000-14000 for continuous dialysis for three days to remove salt ions to obtain a pure silk fibroin aqueous solution.

[0057] Adjust the concentration of silk fibroin solution to 0.15 wt%.

[0058] ...

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Abstract

The invention provides a preparation method of a silk fibroin / metal nanoparticle composite system, belonging to the technical field of nanocomposite materials and preparation methods thereof. The preparation method comprises the steps of fully mixing metal nanoparticles with silk fibroin in a weight ratio of (1:9)-(9:1) so that silk fibroin is non-covalently adsorbed on the surfaces of the metal nanoparticles, thus obtaining the silk fibroin / metal nanoparticle composite system. The metal nanoparticles in the composite system prepared by the preparation method can not aggregate under the conditions that the pH value is 2-11 and the salinity is 0-1000mM, thus improving the stability and biocompatibility of the metal nanoparticles in the physiological environment. The silk fibroin / metal nanoparticle composite system provided by the invention is simple to prepare, is low in cost, has good stability and biocompatibility and has wide application prospects in the biomedical fields of drug sustained release, gene transmission, tissue engineering and the like.

Description

technical field [0001] The invention belongs to the technical field of preparation of nanocomposite materials, and in particular relates to a preparation method of a composite system of silk fibroin and inorganic metal nanoparticles. Background technique [0002] Due to a series of excellent properties, such as magnetic properties, electrical properties, and optical properties, metal nanoparticles have been widely used as nanomaterials in the fields of biology, chemistry, and medicine. In the field of biomedicine, including sustained drug release, gene delivery, biomolecular diagnosis and detection, and biosensors, most of the nanoparticles used in the field require the ability to operate in physiological environments such as a wide pH range and high salt concentration. Stable dispersion in the environment, in addition, biocompatibility is also a prerequisite for the application of nanoparticles. However, metal nanoparticles directly synthesized in aqueous solution usually ...

Claims

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Application Information

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IPC IPC(8): C08L89/00C08K3/08C08K9/10C08K3/22C08J3/05C08J3/11
Inventor 贾兰朱晶心卫爱丽郭丽
Owner TAIYUAN UNIV OF TECH
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