Preparation method of silk fibroin/metal nanoparticle composite system
A technology of metal nanoparticles and silk fibroin, which is applied in the field of preparation of the composite system of silk fibroin and inorganic metal nanoparticles, to achieve the effect of mature extraction technology, wide application range and good stability
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[0023] Embodiment 1:
[0024] (1) Preparation of silk fibroin aqueous solution:
[0025] 20 g of pupated and peeled silkworm cocoons were placed in 2 L of 0.5wt% Na 2 CO 3 In aqueous solution, boiled for 0.5 hours to remove surface sericin, washed three times with deionized water, and dried to obtain degummed silk fibroin fibers. Calcium chloride-ethanol-water (molar ratio 1: 2: 8) ternary system was used to dissolve degummed silk fibroin fibers. The time was 3 h, and the silk fibroin solution was obtained. The solution was vacuum filtered to remove impurities, and then put into a dialysis bag with a molecular weight cut-off of 8000-14000 for continuous dialysis for three days to remove salt ions to obtain a pure silk fibroin aqueous solution. Adjust the concentration of silk fibroin solution to 0.01 wt%.
[0026] (2) Preparation of metal nanoparticles:
[0027] In a 250 mL single-neck flask, add 100 mL of 1 mM HAuCl 4 The solution was heated and boiled, and 9.34 mL ...
Example Embodiment
[0040] Embodiment 2:
[0041] (1) Preparation of silk fibroin aqueous solution:
[0042] Put 20 g of raw silk into 1 L of 1 wt% Na 2 CO 3 In aqueous solution, boiled for 1 hour to remove surface sericin, washed three times with deionized water, and dried to obtain degummed silk fibroin fibers. The degummed silk fibroin was dissolved in a 9 M LiBr aqueous solution. The weight-to-volume ratio of silk fibroin to LiBr aqueous solution was 10:1, the dissolution temperature was 40 °C, and the dissolution time was 2 h to obtain a silk fibroin solution. The silk fibroin solution was vacuum filtered to remove impurities, and then put into a dialysis bag with a molecular weight cut-off of 8000-14000 for continuous dialysis for three days to remove salt ions to obtain a pure silk fibroin aqueous solution. Adjust the silk fibroin solution concentration to 0.05 wt%.
[0043] (2) Prepare an aqueous solution of metal nanoparticles:
[0044] In a 250 mL single-neck flask, add 100...
Example Embodiment
[0054] Embodiment 3:
[0055] (1) Preparation of silk fibroin aqueous solution:
[0056] 50 g of pupa-removed and thinned silkworm cocoons were placed in 3 L of 0.5wt% Na 2 CO 3 In aqueous solution, boiled for 2 hours to remove surface sericin, washed three times with deionized water, and dried to obtain degummed silk fibroin fibers. The degummed silk fibroin fibers were dissolved in a ternary system of calcium chloride-ethanol-water (molar ratio 1: 2: 8). The dissolution time was 1 h to obtain a silk fibroin solution. The solution was vacuum filtered to remove impurities, and then put into a dialysis bag with a molecular weight cut-off of 8000-14000 for continuous dialysis for three days to remove salt ions to obtain a pure silk fibroin aqueous solution.
[0057] Adjust the silk fibroin solution concentration to 0.15 wt%.
[0058] (2) Prepare an aqueous solution of metal nanoparticles:
[0059] In a 250 mL single-neck flask, add 100 mL of 1 mM H 2 PtCl 6 The solution...
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