Method for marking protein polymer by using quantum dots

Abstract

The invention discloses a method for marking a protein polymer by using quantum dots and belongs to the field of a nano-biotechnology. The method is characterized by the following steps: selecting a fluorescent protein dimer (TIP1-mCherry dimer) containing a histidine tag sequence (Histag); and mixing protein and the quantum dots according to a certain ratio and coupling to form a protein-quantum dot composite, wherein the structure of the composite is proved to be very stable through imidazole substitution. According to the method, systematic research is performed on the influence of the assembling characteristic of the protein with a three-dimensional structure and the quantum dots, so the combining stability of the protein and the quantum dots is improved and the marked protein polymer can serve as a nanometer fluorescent probe widely applied to a biomarker.

Description

technical field

[0001] The invention relates to the field of nanobiology technology, in particular to a method for marking protein polymers with quantum dots. Background technique

[0002] Quantum dots (QDs) are zero-dimensional semiconductor nanocrystals, approximately spherical, with a diameter of 1-12nm, and can be dispersed in water or organic solvents to form glue. Compared with traditional organic dyes, QDs have more excellent spectral properties, such as wide and continuous distribution of excitation spectrum, and symmetrical distribution of emission spectrum with narrow width, adjustable color, and high photochemical stability, not easy to photolysis (Marcel BJ , Mario M, Peter G, et al. Science 1998, 281, 2013-2016). These superior properties make QDs widely used in in vivo cell imaging and biological specific labeling. It is and will increasingly become a very important probe tool in molecular cell imaging research.

[0003] At present, fat-soluble CdSe / ZnS quant...

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