Biologically-fermented organic fertilizer and preparation method thereof
A bio-organic fertilizer and bio-fermentation technology, applied in the field of bio-fermentation organic fertilizer and its preparation, to achieve the effect of shortening the fermentation cycle
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Embodiment 1
[0028] The preparation of embodiment 1 bio-organic fertilizer fermentation composite bacterial agent
[0029] 1) Fermentation of Amyloidea CZ1
[0030] Amylomyces CZ1 was inoculated onto the slant of the improved Gaoshi No. 1 solid medium, and cultured at a constant temperature of 35°C for 72 hours to obtain slant seeds;
[0031] The slant seeds were inoculated into the improved Gaoshi No. 1 liquid medium, and cultured at a constant temperature of 35°C and at a speed of 180r / min for 72 hours to obtain liquid seeds;
[0032]Inoculate the liquid seeds into the production medium in the liquid fermenter with 5% (V / V, that is, inoculate 5ml of liquid seeds per 100ml of liquid medium) inoculation amount, and carry out the production of strains, under continuous aeration, at 35°C Cultured at constant temperature for 4 days. The cultured fermented liquid can be used as bacterial agent.
[0033] The composition of the improved Gaoshi No. 1 solid medium is: glucose 10.0g / L, peptone 5...
Embodiment 2
[0046] Embodiment 2 biological organic fertilizer preparation method
[0047] Under the low temperature condition of the ambient temperature of about 5°C, mix fresh cow dung and crushed straw in a certain proportion, adjust the C / N of the pile to 30, and the moisture content to 65%.
[0048] The bacterial agent produced in Example 1 was inoculated into the compost mixture according to the mass ratio of 8‰, and when the compost temperature was higher than 55 degrees, the compost was turned over and ventilated to carry out composting fermentation. Simultaneously, the heap without the bacterial agent of the present invention was used as a control.
[0049] Monitor the temperature change of the compost during the fermentation process, and measure the nutrient content and seed germination index (GI) of the compost after the fermentation is over.
[0050] Determination method: The nutrient content of compost samples was determined according to the agricultural industry standard for...
Embodiment 3
[0059] Fertilizer effect test of embodiment 3 Jingchun baby vegetable organic fertilizer
[0060] Test crops: Jingchun baby cabbage (Brassica campestris L. Beijing Xin No. 3, National Vegetable Engineering Technology Research Center), the growth period is 50 days.
[0061] Experimental site: Experimental field of Beijing Agricultural College. The soil fertility of the tested fields was consistent.
[0062] Experimental design: According to the characteristics of the fertilizer requirement of Jingchun baby cabbage, fertilize according to the application rate of 500 kg / mu of organic fertilizer, apply enough base fertilizer, and do not need topdressing in the later stage. The fertilizer is the organic fertilizer (OF) prepared in Example 2. Set the application of chemical fertilizer (CF) (commercially available urea, diammonium phosphate and potassium sulfate, applied according to the same nutrient content as OF), expanded chicken manure (PJ) (total nutrient content 5%, applied ...
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