Antioxidant peptide of walnut meal and preparation method thereof

A technology of antioxidant peptides and walnut meal, which is applied in chemical instruments and methods, peptides, tripeptides, etc., can solve the problem of wasting high-quality protein resources, and achieve the effects of strong activity, strong antioxidant effect, and small molecular weight

Active Publication Date: 2015-10-28
广东华肽生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

But at present, most of the high-quality walnut meal is directly treated as feed and fertilizer, which wastes a lot of high-quality protein resources.

Method used

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  • Antioxidant peptide of walnut meal and preparation method thereof
  • Antioxidant peptide of walnut meal and preparation method thereof
  • Antioxidant peptide of walnut meal and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026](1) Preparation of enzymatic hydrolyzate of walnut meal protein: mix walnut meal and water at a mass ratio of 1:8, add trypsin with 1% walnut meal protein content, enzymolyze at 55°C for 18 hours; inactivate the enzyme in boiling water for 10 minutes, centrifuge, Take the supernatant and filter it with suction to obtain the enzymatic hydrolyzate of walnut meal protein, the degree of hydrolysis is 9.65±0.37%;

[0027] (2) Separation and purification of antioxidant peptides: after freeze-drying the enzymatic hydrolyzate of walnut meal, use Sephadex G-15 gel column Separation and purification, elution with deionized water at a flow rate of 0.8ml / min, the detection wavelength is 214nm, the antioxidant activity of the components corresponding to each elution peak is measured, the component D with the highest activity is collected, and the highest activity is obtained by freeze-drying powder D;

[0028] (3) After the powder D is dissolved in water, it is purified by reversed...

Embodiment 2

[0031] (1) Preparation of enzymatic hydrolyzate of walnut meal protein: mix walnut meal and water at a mass ratio of 1:10, add trypsin with 2% protein content of walnut meal, enzymolyze at 50°C for 20 hours; inactivate the enzyme in a boiling water bath for 30 minutes, centrifuge, Take the supernatant and filter it with suction to obtain the enzymatic hydrolyzate of walnut meal protein, the degree of hydrolysis is 10.03±0.46%;

[0032] (2) Separation and purification of antioxidant peptides: after freeze-drying the enzymatic hydrolyzate of walnut meal, use Sephadex G-15 gel column For separation and purification, elution was carried out with deionized water at a flow rate of 1.5mL / min, and the detection wavelength was 214nm. The antioxidant activity of the components corresponding to each elution peak was measured, and the component D with the highest activity was collected, and then freeze-dried to obtain the activity highest powder D;

[0033] (3) After the powder D is dis...

Embodiment 3

[0036] Carried out in the same manner as Embodiment 2, except that the enzymatic hydrolysis time was 22 hours to obtain walnut meal protein enzymatic hydrolyzate, the degree of hydrolysis increased to 11.93±0.12%, and the semi-preparative liquid phase elution flow rate was 3mL / min.

[0037] figure 1 Purified antioxidant peptide map for Sephadex G-15 gel filtration chromatography, by figure 2 It can be seen that peak D has the highest antioxidant activity. image 3 For the separation and purification of antioxidant peptides by semi-preparative high performance liquid chromatography, the Figure 4 It can be seen that the antioxidant activity of component D3 is the highest and the activity of component D2 is the second highest, and there is no significant difference between the two components by SPSS analysis (P>0.05).

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Abstract

The invention relates to walnut protein antioxidant peptides and a preparation method thereof. The preparation method comprises: hydrolyzing walnut protein by an enzymatic method to obtain a walnut protein enzymatic hydrolysate, then using gel filtration chromatography and reversed-phase high-performance liquid chromatography to perform separation and purification to obtain fourteen new antioxidant peptide products, and determining the amino acid sequences of the products. The prepared antioxidant peptides have extremely strong antioxidative effect, and high oxygen radical absorption capacity which is 4 times of that of crude peptides; and moreover, the peptides have the characteristics of being low in molecular weight and directly absorbable by a human body, and thus the antioxidant peptides are widely applicable to the fields of foods, healthcare products, cosmetics, and the like.

Description

technical field [0001] The invention relates to the technical field of natural antioxidant peptides, in particular to walnut meal antioxidant peptides and a preparation method thereof. Background technique [0002] Since British Harman proposed the theory of free radicals in 1956, people have gradually realized that many chronic diseases of the human body, such as decreased immunity, cardiovascular diseases, neurodegenerative disorders, cancer and aging, are related to free radicals produced by oxidation in the body, so antioxidants research has attracted more and more attention. Traditionally used antioxidants are mostly chemically synthesized antioxidants, such as di-tert-butylhydroxytoluene (BHT) and tert-butylhydroxyanisole (BHA), which are widely used because they are cheap and have strong antioxidant capacity, but Its use is restricted due to its potential harm to human health. Therefore, finding and developing safe, non-toxic and highly effective natural antioxidant...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/06C07K5/065C07K5/107C07K5/08C07K5/062C07K5/072C07K5/083
Inventor 任娇艳陈卉平赵谋明崔春顾敏蔡孟森
Owner 广东华肽生物科技有限公司
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