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Detection method of vanillin in wine

A detection method and technology of vanillin, applied in measuring devices, instruments, scientific instruments, etc., can solve the problems of many impurities and low recovery rate, and achieve the effects of low detection cost, simple operation and high accuracy

Active Publication Date: 2014-01-08
浙江塔牌绍兴酒有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The determination of vanillin in wine generally adopts gas chromatography. Vanillin has a large solubility difference in water and in organic solvents. During the detection of vanillin by gas chromatography, there will be more impurities due to improper sample handling. , low recovery rate and other reasons affect the accurate quantification

Method used

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  • Detection method of vanillin in wine
  • Detection method of vanillin in wine
  • Detection method of vanillin in wine

Examples

Experimental program
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Embodiment 1

[0020] A method for detecting vanillin in wine according to this embodiment includes the following steps: 1) Preparation of standard solution: Accurately weigh 0.01 g of vanillin powder, add 100 ml of distilled water or deionized water to make up to a concentration of 100 mg / L, and diluted to obtain standard solutions with concentrations of 2.5mg / L, 5mg / L, 10mg / L, 20mg / L, and 40mg / L; Remove ethanol by distillation under reduced pressure. The temperature during distillation under reduced pressure is 40°C. The phenomenon is that the boiling force of the liquid is weakened to stop. Transfer to a 100ml volumetric flask, wash the distillation flask with distilled water or deionized water for 2-3 times, and combine the lotions to volume to 100ml. 3) Measure 2ml of the processed sample in step 2) to a 10ml test tube with a cover, then add 2ml of ether, cover, vortex and mix, let stand and separate the supernatant, and filter with a 45nm organic membrane The processed sample to be t...

Embodiment 2

[0024] A method for detecting vanillin in wine according to this embodiment includes the following steps: 1) Preparation of standard solution: Accurately weigh 0.01 g of vanillin powder, add 100 ml of distilled water or deionized water to make up to a concentration of 100 mg / L, and diluted to obtain standard solutions with concentrations of 2.5mg / L, 5mg / L, 10mg / L, 20mg / L, and 40mg / L; 2) Sample pretreatment: measure 2ml of the wine to be tested 10ml test tube, blow dry with nitrogen at 55°C to remove ethanol. 3) Add 2ml of diethyl ether to the sample processed in step 2), cap it, vortex and mix well, if the layering is difficult, add 0.1-0.3g of analytical pure sodium chloride and let it stand for layering or centrifugal layering, After the supernatant was separated, a 45nm organic membrane was used to filter to obtain a processed sample to be tested. 4) Measure 2ml of the sample processed in step 2), add 100ul of the 100mg / L vanillin standard solution prepared in step 1), th...

Embodiment 3

[0028]A method for detecting vanillin in wine according to this embodiment includes the following steps: 1) Preparation of standard solution: Accurately weigh 0.01 g of vanillin powder, add 100 ml of distilled water or deionized water to make up to a concentration of 100 mg / L, and diluted to obtain standard solutions with concentrations of 2.5mg / L, 5mg / L, 10mg / L, 20mg / L, and 40mg / L; 2) Sample pretreatment: measure 2ml of the wine to be tested 10ml test tube, blow dry with nitrogen at 55°C to remove ethanol. 3) Add 2ml of diethyl ether to the processed sample in step 2), cap it, and vortex to mix it evenly, then let it stand for stratification. Or centrifuge and layer, separate the supernatant, and use 45nm organic membrane filtration to obtain the processed sample to be tested. 4) Measure 2ml of the sample processed in step 2), add 100ul of the 100mg / L vanillin standard solution prepared in step 1), then add 2ml of ether, vortex and mix, let stand and separate When it is di...

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Abstract

The invention discloses a detection method of vanillin in wine. The detection method of vanillin in wine comprises the following steps:1) preparing a standard liquid; 2) pretreating a sample, namely, weighing a sample of to-be-detected wine, and removing alcohol; 3) weighing the sample treated in the step 2), and extracting by adopting diethyl ether; 4) weighing the sample treated in the step 2), adding the configured standard liquid in the step 1), and extracting again by adopting diethyl ether; and 5) detecting the standard liquid prepared in the step 1), the sample treated in the step 3) and the sample treated in the step 4) by adopting a gas chromatograph. According to the detection method disclosed by the invention, the vanillin in wine can be extracted fully by a diethyl ether liquid-liquid extraction mode so as to achieve impurity removal and enrichment to conform to the detection requirement by a gas chromatographic (GC) method, and accurate quantification is realized through an external standard method by the standard sample recovery rate correcting and extracting efficiency.

Description

technical field [0001] The invention relates to a detection method of vanillin, in particular to a detection method of vanillin in wine. Background technique [0002] Vanillin, also known as vanillin, is a widely used edible spice, which can be found in the seeds of vanilla or artificially synthesized. It has a strong milky smell. Natural vanillin is also found in some Among fermented wines, it is one of the important components of wine aroma components. The determination of vanillin in wine generally adopts gas chromatography. Vanillin has a large solubility difference in water and in organic solvents. During the detection of vanillin by gas chromatography, there will be more impurities due to improper sample handling. , Low recovery and other reasons affect the accurate quantification. Contents of the invention [0003] The object of the present invention is to provide a method for detecting vanillin in wine, which can fully extract the vanillin in wine by means of eth...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02
Inventor 单之初俞红波曹秀苗沈翔
Owner 浙江塔牌绍兴酒有限公司
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