Fluorescent microsphere immunochromatographic assay quantitative detector by combination of internet-of-things data transmission system

Abstract

The invention belongs to the field of immunodetection instruments and discloses a fluorescent microsphere immunochromatographic assay quantitative detection device based on an internet-of-things data transmission system. The fluorescent microsphere immunochromatographic assay quantitative detection device comprises a fluorescent microsphere immunochromatographic assay quantitative reader, a fluorescent microsphere immunochromatographic assay test strip and the internet-of-things data transmission system; the detection device skillfully combines an immunochromatographic technology with the internet-of-things data transmission system, has unique advantages, rapidness in detection, small volume, light weight and convenience for carrying, can independently process data and achieves the purpose of quantitative detection, and furthermore, the detection device combines the powerful data management capacity of the internet-of-things data transmission system, realizes the on-site analysis and the retrospective analysis on detected data and is convenient to operate and control the detection results in real time by a data management center.

Description

technical field [0001] The invention relates to an immunological detection device, in particular to a fluorescent microsphere immunochromatography quantitative detection instrument. Background technique [0002] Immunochromatography (immunochromatography) technology is a rapid immunoassay method developed in the 1980s that combines immune technology and chromatographic technology. In recent years, it has been widely used in the field of biological and medical detection at home and abroad. The principle is to use the strip-shaped fiber chromatography material (such as nitrocellulose membrane) as the solid phase, and fix the specific antibody (or antigen) on a certain zone of the strip-shaped fiber chromatography material, when the dry nitric acid After immersing one end of the cellulose in the sample solution (urine or serum), the sample solution swims on the chromatographic strip by means of capillary action, and at the same time, the analyte in the sample and the receptor ...

AI Technical Summary

Problems solved by technology

[0003] (1) The process of antibody-labeled colloidal gold belongs to the process of electrostatic adsorption, which belongs to non-specific physical adsorption, which easily leads to the desorption of antibodies from the surface of colloidal gold particles;

[0004] (2) Only when the colloidal gold particles gather to a certain amount can the bands visible to the naked eye be displayed, so the detection sensitivity is not high;

[0005] (3) The matrix effect of different materials is obvious, and the background interference is very large;

[0006] (4) Colloidal gold immunochromatography technology generally can only achieve directional or semi-quantitative detection

Therefore, the realization of quantitative detection is of great significance. At present, there are methods that can complete quantitative detection on the market, such as gas chromatography, liquid chromatography, etc., but such methods need the help of corresponding detection instruments, and the detection time is long. Although fully automatic The detection instrument can realize rapid detection in the face of a large number of test samples, but its main disadvantages are expensive, complicated sample pretreatment, professional operation is required, and the instrument is heavy, which is not suitable for on-site and grassroots use

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