Method for reducing ammonia nitrogen content in polymyxin E fermentation liquor

A technology of polymyxin and ammonia nitrogen content, applied in the direction of microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve problems such as unfavorable long-term development of enterprises, increase the burden of sewage treatment, etc., and achieve the effect of reducing difficulty

Active Publication Date: 2014-01-15
HEBEI SHENGXUE DACHENG PHARMA
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  • Application Information

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Problems solved by technology

Although this method can control the pH and supplement the inorganic nitrogen source, it brings a large amount of ammonia nitrogen to the sewage treatment in the later stage of feeding, which increases the burden of sewage treatment. In the case of increasingly severe environmental protection situation, it is not conducive to the long-term development of enterprises. Achieving unification of economic, social and environmental benefits

Method used

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  • Method for reducing ammonia nitrogen content in polymyxin E fermentation liquor

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Effect test

Embodiment 1

[0026] A 50L Bailun stainless steel fermentation tank is filled with 30L fermentation broth, inoculated with 2-5% seed broth, and after 8 hours of transfer from the fermentation broth, the ammonia water is added to automatically control the pH between 5.6-6.6 and the fermentation is completed. The fermentation effect of polymyxin E is The price is 512,000 U / mL, and the ammonia nitrogen content of the tank in the fermentation broth is about 500mg / 100mL.

Embodiment 2

[0028] A 50L stainless steel fermentation tank is filled with 30L of fermentation broth, inoculated with 2-5% of the seed liquid, after 15 hours of transfer from the fermentation broth, the flow of ammonia is automatically controlled to pH 5.6, and after 60 hours of transfer from the fermentation broth, the flow of ammonia is stopped. The pH was controlled to 5.6 by adding 10% sodium hydroxide solution. After 30 hours of adding, the fermentation titer of polymyxin E was 504,000 U / mL. Compared with the existing technology, the amount of ammonia water is reduced by about 30%, and the ammonia nitrogen content in the fermentation broth is reduced from about 500mg / 100mL to about 300mg / 100mL.

Embodiment 3

[0030] A 50L stainless steel fermentation tank is filled with 30L fermentation broth, inoculated with 2-5% of the seed broth, and after 10 hours of transfer from the fermentation broth, the flow of ammonia is automatically controlled to pH 6.0, and after 30 hours of transfer from the fermentation broth, the flow of ammonia is stopped. To control the pH to 6.0 by adding 20% ​​sodium hydroxide solution, and then adding for 60 hours, the polymyxin E fermentation potency was 518,000 U / mL. Compared with the existing technology, the amount of ammonia water is reduced by about 32%, and the ammonia nitrogen content in the fermentation broth is reduced from about 500mg / 100mL to about 260mg / 100mL.

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Abstract

The invention discloses a method for reducing the ammonia nitrogen content in polymyxin E fermentation liquor. The method comprises the step of dropwise adding an alkaline substance during the fermentation process of polymyxin E to control the pH value of the fermentation liquor. The key point is that the method is to feed ammonia water and sodium hydroxide sequentially at the beginning of fermentation to control the pH value of the fermentation liquor. The beneficial effects are as follows: when the polymyxin E is fermented for a certain time, the sodium hydroxide is fed to replace the ammonia water to maintain the pH value, so that the nitrogen source is supplemented, the pH value is controlled, and the ammonia nitrogen content in the polymyxin E waste fermentation liquor can be reduced by more than 40%. Therefore, the difficulty of sewage treatment is reduced from the source, and the clean production requirement of polymyxin E fermentation can be met.

Description

technical field [0001] The invention belongs to the field of biochemical technology. The invention relates to a method for reducing the concentration of ammonia nitrogen in polymyxin E fermentation liquid, specifically a method for producing polymyxin E by feeding different alkaline substances. Background technique [0002] Polymyxin E is produced by Paenibacillus polymyxa (Bacillus polymgxa), a basic polypeptide antibiotic composed of various amino acids and fatty acids, and has a strong bactericidal effect on Gram-negative bacilli. Polymyxin E has good antibacterial properties, high efficiency, low toxicity, and less residue. It is widely used in feed additives, and can promote the growth of poultry and livestock and improve feed utilization. [0003] However, at present, the method of adding ammonia water throughout the fermentation process is generally used to control the pH of the fermentation broth. Although this method can control the pH and supplement the inorganic...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/04C12R1/12
Inventor 魏伟张宝荣王绘砖李琪
Owner HEBEI SHENGXUE DACHENG PHARMA
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