Method for reducing ammonia nitrogen content in polymyxin E fermentation liquor

A technology of polymyxin and ammonia nitrogen content, applied in microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve problems such as unfavorable long-term development of enterprises and increase the burden of sewage treatment, and achieve the effect of reducing difficulty

Active Publication Date: 2015-02-25
HEBEI SHENGXUE DACHENG PHARMA
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AI Technical Summary

Problems solved by technology

Although this method can control the pH and supplement the inorganic nitrogen source, it brings a large amount of ammonia nitrogen to the sewage treatment in the later stage of feeding, which increases the burden of sewage treatment. In the case of increasingly severe environmental protection situation, it is not conducive to the long-term development of enterprises. Achieving unification of economic, social and environmental benefits

Method used

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  • Method for reducing ammonia nitrogen content in polymyxin E fermentation liquor

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Effect test

Embodiment 1

[0026] Put 30L of fermentation liquid into a 50L hundred-lun stainless steel fermenter, inoculate 2-5% seed liquid, and then add ammonia water to automatically control the pH to 5.6-6.6 until the end of fermentation. The fermentation effect of polymyxin E The price is 512,000 U / mL, and the ammonia nitrogen content in the fermentation broth is about 500mg / 100mL.

Embodiment 2

[0028] Put 30L of fermented liquid into a 50L hundred-lun stainless steel fermenter, inoculate with 2-5% seed liquid, add ammonia water to automatically control the pH to 5.6 after 15 hours from the inoculation of the fermented liquid, and stop the flow of ammonia water after 60 hours of inoculation from the fermented liquid. In order to control the pH to 5.6 by adding 10% sodium hydroxide solution, after 30 hours of feeding, the fermentation titer of polymyxin E was 504,000 U / mL. Compared with the existing technology, the amount of ammonia water is reduced by about 30%, and the ammonia nitrogen content in the fermentation broth is reduced from about 500mg / 100mL to about 300mg / 100mL.

Embodiment 3

[0030] Put 30L of fermented liquid into a 50L hundred-lun stainless steel fermenter, inoculate with 2-5% seed liquid, add ammonia water to automatically control the pH to 6.0 after 10 hours of inoculation from the fermented liquid, and stop the flow of ammonia water after 30 hours of inoculation from the fermented liquid. In order to control the pH to 6.0 by feeding 20% ​​sodium hydroxide solution, after feeding for 60 hours, the fermentation titer of polymyxin E was 518,000 U / mL. Compared with the existing technology, the amount of ammonia water is reduced by about 32%, and the ammonia nitrogen content in the fermentation broth is reduced from about 500mg / 100mL to about 260mg / 100mL.

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Abstract

A method for reducing the ammonia nitrogen content in the polymyxin E fermentation liquid, comprising the step of controlling the pH of the fermentation liquid by dropping alkaline substances during the fermentation of the polymyxin E, the key is: the method is in After the fermentation starts, ammonia water and sodium hydroxide are used to feed successively to control the pH of the fermentation liquid. The beneficial effect of the present invention is that, after fermentation reaches a certain period of time, the pH can be maintained by feeding sodium hydroxide solution instead of ammonia water, which can not only achieve The purpose of supplementing nitrogen source and controlling pH can also reduce the content of ammonia nitrogen in polymyxin E fermentation waste liquid by more than 40%, which reduces the difficulty of sewage treatment from the source and achieves the cleanliness of polymyxin E fermentation. Production requirements.

Description

Technical field [0001] The present invention belongs to the field of biochemical technology.It involves a method that reduces the concentration of ammonia nitrogen concentration in the fermentation solution of polyphonoh, and the method of producing multi -viscosin E is specifically produced by flowing different alkaline substances. Background technique [0002] Polyphonin E is a alkaline polypeptide antibiotic consisting of Bacillus Polymgxa and consisting of a variety of amino acids and fatty acids, which has a strong sterilization effect on Gram -negative bacteria.Multi -muin E has the characteristics of good antibacterial and efficient, low toxic, and residue. It is widely used for feed additives, and can promote the growth of poultry and raising feed. [0003] However, the pH of the fermentation liquid is generally used to control the fermentation liquid during the fermentation process.Although this method can control pH and supplement the source of inorganic nitrogen, it br...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P21/04C12R1/12
Inventor 魏伟张宝荣王绘砖李琪
Owner HEBEI SHENGXUE DACHENG PHARMA
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