Treatment method of urine in cancer detection process
A treatment method and cancer detection technology, which is applied in the field of biomedicine and early molecular diagnosis of cancer, can solve the problems of large samples, environmental pollution, etc., and achieve the effect of reducing the amount of solvent, reducing the test time, and small sample volume
Inactive Publication Date: 2014-01-15
JIANGSU HEERSI BIOTECH
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Problems solved by technology
[0009] Purpose of the invention: In order to solve the problem of huge pollution to the environment due to th
Method used
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[0012] specific implementation plan
[0013] The treatment method of the urine sample in the process of cancer detection, after the sample is received, it is quickly stored in a -80 degree Celsius refrigerator; the urine sample is transferred to a mass / volume of 3% potassium iodide (KI) and 3% iodine (I2) 2 ml sample In the jar, shake gently and let it stand for 5 minutes, then add 200 microliters of 1 mole per liter of sodium hydroxide and 1 mole of sodium chloride into the 2 ml sample jar, mix well and then place it in a 4-degree refrigerator for 15 minutes, centrifuge at 8000 rpm for 10 minutes in a refrigerated centrifuge; transfer 100 microliters of the supernatant to a 2 milliliter sample jar of reagent C for detection.
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The invention discloses a treatment method of urine in a cancer detection process. The treatment method comprises the following steps: rapidly putting a sample into -80 DEG C refrigerator for preservation; transferring the urine to a 2 ml sample tank with 3% of potassium iodide (KI) and 3% of iodine (I2) in mass/ volume, and lightly rocking and standing for 5 minutes, adding 200 microliters of 1mol/ L sodium hydroxide and 1mol/ L sodium chloride into the 2 ml sample tank, sufficiently mixing, putting into a 4 DEG C refrigerator for 15 minutes, and centrifuging 10 minutes in a freezing centrifuge at 8000 rpm; transferring 100 microliters of a supernatant to a 2 ml sample tank with a reagent C for detection. The treatment method has the advantages as follows: the amount of the needed solvent is reduced; the amount of the needed sample is relatively small; the sample treating process is simplified; the entire detection time is shortened.
Description
technical field [0001] The invention relates to a method for processing urine samples during cancer detection, and belongs to the technical field of biomedicine, in particular to the field of early molecular diagnosis of cancer. Background technique [0002] Cancer is the second leading cause of death globally and is responsible for around 13% of all deaths worldwide. Cancer mortality by type of cancer per year is cancer (1.4 million cases / year), gastric cancer (866,000 cases / year), liver cancer (653,000 cases / year), colon cancer (677,000 cases / year), and breast cancer (548,000 cases / year ). Cancer deaths worldwide are projected to continue to rise, with an estimated 12 million deaths from cancer in 2030. These statistics fully demonstrate the density and intensity of cancer occurrence on a global scale. Many research institutes and scientists focus on cancer research because it is one of the most serious health problems in the modern world. [0003] Cancer occurs when ce...
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Inventor 成晓亮季军马银法
Owner JIANGSU HEERSI BIOTECH
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