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A method for activating oil reservoir endogenous microorganisms to produce emulsifier

A technology of bioemulsifier and endogenous microorganisms, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, chemical instruments and methods, etc., can solve the problem that the effect of emulsifying microbial oil displacement has not been fully and effectively exerted, etc. Achieve the effects of formula system optimization, strong operability and strong pertinence

Active Publication Date: 2016-02-10
CHINA PETROLEUM & CHEM CORP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the ecological complexity of the endogenous microbial population in the oil reservoir, the oil displacement effect of the emulsifying microorganisms has not been fully and effectively exerted.

Method used

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  • A method for activating oil reservoir endogenous microorganisms to produce emulsifier
  • A method for activating oil reservoir endogenous microorganisms to produce emulsifier
  • A method for activating oil reservoir endogenous microorganisms to produce emulsifier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Taking Block A3 of Shengli Oilfield as an example

[0026] The burial depth of this reservoir is 1162m~1350m, the reservoir temperature is 65℃, the coefficient of variation of permeability is 0.528, the porosity is 35%, and the air permeability is 1600×10 -3 μm 2 , the oil-bearing area of ​​the test area is 0.83km 2 , effective thickness 10.1m, geological reserves 154.9×10 4 t, implementing the steps of the present invention is:

[0027] 1. Analysis of the produced emulsifier microbial population in reservoir samples

[0028] Quantitative analysis of microbial emulsification functional genes was carried out on the reservoir samples, and the results are shown in Table 1.

[0029] Table 1 Quantitative analysis of emulsification function genes of reservoir samples in block A3

[0030]

[0031] The analysis results showed that the number of protein tyrosine phosphatase genes in the injected water and the produced fluid of the three oil wells exceeded 200c...

Embodiment 2

[0066] Embodiment 2: Take the L block of Shengli Oilfield as an example

[0067] The burial depth of this reservoir is 838m~1628m, the formation temperature is 50℃, the average porosity is 28.5%, and the average permeability is 1212×10 -3 μm 2 , the original oil saturation is 63%. Utilize the method of the present invention to activate the emulsifier of endogenous microorganisms in oil reservoirs to carry out microbial oil displacement test, concrete steps are as follows:

[0068] 1. Analysis of the produced emulsifier microbial population in reservoir samples

[0069] Selective culture of microorganisms and quantitative analysis of functional genes were carried out on the reservoir samples, and the results are shown in Table 8.

[0070] Table 8 Selective culture and quantitative analysis of functional genes of reservoir samples in block L

[0071]

[0072] The analysis results in Table 8 show that the number of protein tyrosine phosphatase genes in the oil reservoir sa...

Embodiment 3

[0098] Embodiment 3: Take the M block of Shengli Oilfield as an example

[0099] The burial depth of this reservoir is 1920-2030m, the formation temperature is 98°C, the average porosity is 28.8%, and the average permeability is 315×10 -3 μm 2 , the original oil saturation is 50%. Utilize the method of the present invention to activate the emulsifier of endogenous microorganisms produced in oil reservoirs to carry out microbial oil displacement test, the specific steps are as follows:

[0100] 1. Analysis of the produced emulsifier microbial population in reservoir samples

[0101] Selective culture of microorganisms and quantitative analysis of functional genes were carried out on the reservoir samples, and the results are shown in Table 12.

[0102] Table 12 Quantitative analysis of functional genes of reservoir samples in block 2M

[0103]

[0104]

[0105]From Table 12, it can be seen that for the M block reservoir sample, the result of functional gene detection ...

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Abstract

The invention relates to the technical field of microbial enhanced oil recovery, in particular to a method for improving the enhance oil recovery by selectively activating microorganisms to drive oil pool indigenous microorganisms to generate bio-emulsifier. The method comprises the following steps that (A) microbial populations generating the bio-emulsifier in oil pool samples are analyzed; (B) the microbial populations generating the bio-emulsifier and the key metabolism substrates of the microbial populations generating the bio-emulsifier are determined, and an activating agent formula system is designed; (C) the activating agent formula system is optimized; (D) the field injection technology of activating agents is optimized; (E) oil filed injection tests of the activating agents are carried out. The method has the advantages of being strong in pertinence, high in reliability, wide in oil pool application range, simple in method, strong in operability, good in on-site implementation effect and the like, and therefore the method can be widely applied to the microorganism oil displacement field tests.

Description

technical field [0001] The invention relates to the technical field of microbial oil recovery, in particular to a method for enhancing oil recovery by selectively activating an emulsifier produced by endogenous microorganisms in oil reservoirs. Background technique [0002] One of the principles of microbial oil flooding is to use its metabolites to achieve. There are many kinds of metabolites of microorganisms, among which bioemulsifier is a high molecular weight biosurfactant produced by microorganisms during their growth and metabolism. Such substances can form stable emulsions with hydrophobic substances such as petroleum hydrocarbons, reduce interfacial tension, increase the fluidity of crude oil, and have low toxicity and biodegradability. [0003] At present, bioemulsifier flooding mainly adopts two methods: injecting bioemulsifier fermentation products into oil reservoirs and producing emulsifier exogenous microorganisms. The high fermentation cost of the former is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): E21B43/22C12Q1/68C12Q1/64C12Q1/04C09K8/584
Inventor 张绍东宋永亭李彩风汪卫东郭省学曹嫣镔郭辽原高光军郝滨吴晓玲
Owner CHINA PETROLEUM & CHEM CORP
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