A method for activating oil reservoir endogenous microorganisms to produce emulsifier
A technology of bioemulsifier and endogenous microorganisms, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, chemical instruments and methods, etc., can solve the problem that the effect of emulsifying microbial oil displacement has not been fully and effectively exerted, etc. Achieve the effects of formula system optimization, strong operability and strong pertinence
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Embodiment 1
[0025] Example 1: Taking Block A3 of Shengli Oilfield as an example
[0026] The burial depth of this reservoir is 1162m~1350m, the reservoir temperature is 65℃, the coefficient of variation of permeability is 0.528, the porosity is 35%, and the air permeability is 1600×10 -3 μm 2 , the oil-bearing area of the test area is 0.83km 2 , effective thickness 10.1m, geological reserves 154.9×10 4 t, implementing the steps of the present invention is:
[0027] 1. Analysis of the produced emulsifier microbial population in reservoir samples
[0028] Quantitative analysis of microbial emulsification functional genes was carried out on the reservoir samples, and the results are shown in Table 1.
[0029] Table 1 Quantitative analysis of emulsification function genes of reservoir samples in block A3
[0030]
[0031] The analysis results showed that the number of protein tyrosine phosphatase genes in the injected water and the produced fluid of the three oil wells exceeded 200c...
Embodiment 2
[0066] Embodiment 2: Take the L block of Shengli Oilfield as an example
[0067] The burial depth of this reservoir is 838m~1628m, the formation temperature is 50℃, the average porosity is 28.5%, and the average permeability is 1212×10 -3 μm 2 , the original oil saturation is 63%. Utilize the method of the present invention to activate the emulsifier of endogenous microorganisms in oil reservoirs to carry out microbial oil displacement test, concrete steps are as follows:
[0068] 1. Analysis of the produced emulsifier microbial population in reservoir samples
[0069] Selective culture of microorganisms and quantitative analysis of functional genes were carried out on the reservoir samples, and the results are shown in Table 8.
[0070] Table 8 Selective culture and quantitative analysis of functional genes of reservoir samples in block L
[0071]
[0072] The analysis results in Table 8 show that the number of protein tyrosine phosphatase genes in the oil reservoir sa...
Embodiment 3
[0098] Embodiment 3: Take the M block of Shengli Oilfield as an example
[0099] The burial depth of this reservoir is 1920-2030m, the formation temperature is 98°C, the average porosity is 28.8%, and the average permeability is 315×10 -3 μm 2 , the original oil saturation is 50%. Utilize the method of the present invention to activate the emulsifier of endogenous microorganisms produced in oil reservoirs to carry out microbial oil displacement test, the specific steps are as follows:
[0100] 1. Analysis of the produced emulsifier microbial population in reservoir samples
[0101] Selective culture of microorganisms and quantitative analysis of functional genes were carried out on the reservoir samples, and the results are shown in Table 12.
[0102] Table 12 Quantitative analysis of functional genes of reservoir samples in block 2M
[0103]
[0104]
[0105]From Table 12, it can be seen that for the M block reservoir sample, the result of functional gene detection ...
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