Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for activating oil pool indigenous microorganisms to generate bio-emulsifier

A technology of biological emulsifier and endogenous microorganisms, which is applied in the direction of biochemical equipment and methods, microbial measurement/inspection, chemical instruments and methods, etc., can solve the problem that the effect of emulsifying microbial oil displacement has not been fully and effectively exerted, etc. Achieve the effect of formula system optimization, simple method and good field test effect

Active Publication Date: 2014-01-22
CHINA PETROLEUM & CHEM CORP +1
View PDF4 Cites 14 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to the ecological complexity of the endogenous microbial population in the oil reservoir, the oil displacement effect of the emulsifying microorganisms has not been fully and effectively exerted.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for activating oil pool indigenous microorganisms to generate bio-emulsifier
  • Method for activating oil pool indigenous microorganisms to generate bio-emulsifier
  • Method for activating oil pool indigenous microorganisms to generate bio-emulsifier

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0025] Example 1: Take Block A3 of Hekou Oil Production Plant in Shengli Oilfield as an example

[0026] The burial depth of the reservoir is 1162m-1350m, the reservoir temperature is 65°C, the permeability coefficient of variation is 0.528, the porosity is 35%, and the air permeability is 1600×10 -3 μm 2 , the oil-bearing area of ​​the test area is 0.83km 2 , the effective thickness is 10.1m, and the geological reserves are 154.9×10 4 t, the step of implementing the present invention is:

[0027] 1. Analysis of bio-emulsifier-producing microbial populations in reservoir samples

[0028] Quantitative analysis of microbial emulsification function genes was carried out on the reservoir samples, and the results are shown in Table 1.

[0029] Table 1A3 Quantitative analysis of emulsifying function genes of reservoir samples from block A3

[0030]

[0031] The analysis results show that the number of protein tyrosine phosphatase genes in the injected water and the produced ...

Embodiment 2

[0068] Example 2: Take L block of Gudao Oil Production Plant in Shengli Oilfield as an example

[0069] The burial depth of the reservoir is 838m~1628m, the formation temperature is 50℃, the average porosity is 28.5%, and the average permeability is 1212×10 -3 μm 2 , the original oil saturation is 63%. The method of activating endogenous microorganisms of the present invention to produce biological emulsifier has carried out a microbial oil flooding test, and the specific steps are as follows:

[0070] 1. Analysis of bio-emulsifier-producing microbial populations in reservoir samples

[0071] Microbial selective culture and quantitative analysis of functional genes were carried out on the reservoir samples. The results are shown in Table 8.

[0072] Table 8. Selective culture and quantitative analysis of functional genes of L block reservoir samples

[0073]

[0074]

[0075] The analysis results in Table 8 show that the number of protein tyrosine phosphatase genes i...

Embodiment 3

[0102] Example 3: Taking Block M of Hekou Oil Production Plant in Shengli Oilfield as an example

[0103] The burial depth of the reservoir is 1920-2030m, the formation temperature is 98°C, the average porosity is 28.8%, and the average permeability is 315×10 -3 μm 2 , the original oil saturation is 50%. Utilize the method for activating endogenous microorganisms in the oil reservoir to produce biological emulsifier to carry out the microbial oil flooding test, and the specific steps are as follows:

[0104] 1. Analysis of bio-emulsifier-producing microbial populations in reservoir samples

[0105]Selective culture of microorganisms and quantitative analysis of functional genes were carried out on the reservoir samples. The results are shown in Table 12.

[0106] Table 12 Quantitative analysis of functional genes in block M reservoir samples

[0107]

[0108] It can be seen from Table 12 that for the M block of oil reservoir samples, the result of functional gene detect...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Depthaaaaaaaaaa
Average permeabilityaaaaaaaaaa
Average permeabilityaaaaaaaaaa
Login to View More

Abstract

The invention relates to the technical field of microbial enhanced oil recovery, in particular to a method for improving the enhance oil recovery by selectively activating microorganisms to drive oil pool indigenous microorganisms to generate bio-emulsifier. The method comprises the following steps that (A) microbial populations generating the bio-emulsifier in oil pool samples are analyzed; (B) the microbial populations generating the bio-emulsifier and the key metabolism substrates of the microbial populations generating the bio-emulsifier are determined, and an activating agent formula system is designed; (C) the activating agent formula system is optimized; (D) the field injection technology of activating agents is optimized; (E) oil filed injection tests of the activating agents are carried out. The method has the advantages of being strong in pertinence, high in reliability, wide in oil pool application range, simple in method, strong in operability, good in on-site implementation effect and the like, and therefore the method can be widely applied to the microorganism oil displacement field tests.

Description

technical field [0001] The invention relates to the technical field of microbial oil recovery, in particular to a method for improving crude oil recovery by selectively activating endogenous microorganisms in an oil reservoir to produce a biological emulsifier. Background technique [0002] One of the principles of microbial oil flooding is to use its metabolites to achieve. There are many metabolites of microorganisms, among which bioemulsifier is a high molecular weight biosurfactant produced by microorganisms in the process of growth and metabolism. These substances can interact with hydrophobic substances such as petroleum hydrocarbons to form stable emulsions, reduce interfacial tension, increase the fluidity of crude oil, and also have the characteristics of low toxicity and biodegradation. [0003] At present, bio-emulsifier flooding mainly adopts two methods: injecting bio-emulsifier fermentation products into reservoirs and producing emulsifier exogenous microorgan...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): E21B43/22C12Q1/68C12Q1/64C12Q1/04C09K8/584
Inventor 张绍东宋永亭李彩风汪卫东郭省学曹嫣镔郭辽原高光军郝滨吴晓玲
Owner CHINA PETROLEUM & CHEM CORP
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com