Method for fast identifying key quasi/anti-androgen interference poison in water sample

An anti-androgen and androgen technology, applied in the field of pretreatment and measurement, can solve the problems of high cost and difficulty in identifying key androgen interferents, and achieve the effects of low cost, fewer steps, and less consumption of reagents
CN103529145AActive Publication Date: 2014-01-22NANJING UNIV
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
NANJING UNIV
Publication Date
2014-01-22

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Abstract

The invention discloses a method for fast identifying key quasi / anti-androgen interference poison in a water sample. The method mainly comprises the following steps that enrichment is carried out on the water sample through solid-phase extraction columns, and negative-pressure hollow drying is carried out on the extraction columns; the extraction columns are eluted through organic solvents in sequence, concentration and volume measurement are carried out on eluent, one half of the eluent is used for the toxicity test of quasi / anti-androgens, the total toxic equivalent is calculated based on a standard substance EC50 and the dilution ratio, and the other half of the eluent is used for identifying the main toxic substances of a toxic sample; based on the spectrum signature and the toxic equivalent of common quasi / anti-androgen active poison, a poison mass balance database is built, then the toxic equivalent and the toxic contribution rate are calculated based on the results of qualitative analysis and quantitative analysis, and the main toxic substances are determined. According to the method, the number of demanded samples is small, reagent consumption is little, cost is low, the number of the steps is small, and the quasi / anti-androgen poison in water bodies with different pollution levels can be identified fast.
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Description

technical field

[0001] The invention relates to a pretreatment and determination method for rapid identification of pseudo / anti-androgen interference poisons in water bodies with different pollution degrees, more specifically, it is a method that combines chemical analysis and toxic equivalent calculation, which is aimed at containing common pseudo / androgen interference poisons. The complex environmental water samples of anti-androgen interference active substances are extracted with the same column, and then based on the mass balance database, the established toxic equivalent calculation method and the SRM scanning mode of GC-MSMS are used to quickly identify the source of toxicity for toxicant screening. Background technique

[0002] With the development of the economy and the continuous improvement of people's living standards, a large number of organic pollutants are continuously discharged into the water body environment in the process of industrial and agricultural prod...

Claims

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