DNA molecular machine and single-base mutation detection method based on DNA molecular machine as well as application of method

Abstract

The invention provides a DNA molecular machine and also provides a single-base mutation detection method based on the DNA molecular machine. The method comprises the following steps: initiating different running capabilities of the DNA molecular machine by utilizing different catalytic chains, combining assembly of gold nanoparticles, observing the reaction difference of a catalytic chain system in which completely complementary catalytic chains are added and the catalytic chain system with single-base mutation through a contrast experiment, and finally realizing the single-base mutation detection based on the DNA molecular machine. Strict operating conditions are not needed in the detection means, and the operation is simple. The detection means of the signal is simple and can be identified through colors and ultraviolet spectrums in actual application. The difficulty that complex operation exists or a precise instrument needs to be used for observation in a conventional detection technology is solved. The detected base mutation type is comprehensive and comprises all base mutation forms, namely base mismatch, base deletion and base insertion, and a mutation position of the detected DNA chain is random.

Description

technical field [0001] The invention relates to the technical field of DNA molecular detection technology, in particular to a DNA molecular machine, a single base mutation detection method based on the DNA molecular machine and an application thereof. Background technique [0002] Single-base mutation is a ubiquitous gene variation type, and almost 1% of alleles in the human genome will have single-base mutation. The detection of single base mutations is now attracting more and more attention, because the variation in the human DNA sequence has great significance for the diagnosis of diseases and the design of drugs. [0003] So far, there are many methods to realize single base mutation detection, among which the important methods are enzyme-assisted method and DNA hybridization method. The enzyme-assisted method can be operated at room temperature and has high sensitivity and strong specificity, but the operation process is complicated, and the specificity of the reaction...

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Problems solved by technology

[0005] Therefore, in order to overcome the difficulties in the detection of single-base mutations in DNA in the prior art, the inventors have conducted in-depth research and found that the detection of single-base mutations can be realized under simple operations based on DNA molecular machines combined with the assembly of gold nanoparticles. Detection, in the further search of the existing technical literature, there is no experiment that triggers the DNA molecular machine through the catalytic chain with a single base mutation at room temperature to promote the assembly of gold nanoparticles. In addition, there is no experiment that combines DNA molecular machines with gold nanoparticles. A report on the detection of single base mutations by controlled assembly

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